ICEberg ID103
OrganismClostridium difficile 630
Size (bp)20657
GC content [Genome] (%)38.3 [29]
Insertion siteAT rich regions
FunctionTetracycline resistance
Species that ICE can be transferred toBacillus subtilis; Clostridium difficile
Nucleotide SequenceAM180355 (complete ICE sequence in this genome)
Replicon chromosome (4290252 bp, Project:78) [NC_009089]
Genome coordinates585384..606040
Link to view genome context of this ICE in the genome browser
experimental This is a ICE derived from experimental literature.
Complete gene list of Tn5397 form AM180355
#GeneCoordinates [+/-], size (bp)Protein GI Product *
1CD630_04900580547..581599 [+], 1053115249504 putative sugar-phosphate dehydrogenase
2CD630_04910581655..582062 [+], 408115249505 PTS system, mannose/fructose/sorbose IIA component
3CD630_04920582091..582564 [+], 474115249506 PTS system, mannose/fructose/sorbose IIB component
4CD630_04930582611..583369 [+], 759115249507 PTS system, mannose/fructose/sorbose IIC component
5CD630_04940583393..584232 [+], 840115249508 PTS system, mannose/fructose/sorbose IID component
6CD630_04950584769..606563 [+], 21795pseudogene Fragment of putative regulatory protein
7CD630_04960585695..586009 [+], 315115249509  [UniProt] putative conjugative transposon protein DUF961 family Tn5397, CTn3-Orf23
8CD630_04970586031..586417 [+], 387115249510  [UniProt] putative conjugative transposon protein DUF961 family Tn5397, CTn3-Orf22
9CD630_04980586451..587836 [+], 1386115249511  [UniProt] putative cell-division FtsK/SpoIIIE-family protein Tn5397, CTn3-Orf21
10CD630_04981587839..587991 [+], 153115249512  [UniProt]  [KEGG]putative conjugative transposon protein Tn5397,CTn3-Orf20.1
11CD630_04990588010..589218 [+], 1209115249513  [UniProt]  [KEGG]putative replication initiation factor Tn5397,CTn3-Orf20
12CD630_04991589261..589482 [+], 222115249514  [UniProt] putative conjugative transposon protein Tn5397,CTn3-Orf19
13CD630_05000589599..590096 [+], 498115249515  [UniProt] putative antirestriction protein Tn5397,CTn3-Orf18
14CD630_05010590184..590576 [+], 393115249516  [UniProt] putative conjugative transposon protein Tn5397,CTn3-Orf17
15CD630_05020590560..593007 [+], 2448115249517  [UniProt]  [KEGG]putative ATPase Tn5397, CTn3-Orf16
16CD630_05030593007..595175 [+], 2169115249518  [UniProt] putative membrane protein Tn5397, CTn3-Orf15
17CD630_05040595172..598820 [+], 3649pseudogene Fragment of putative conjugative transposon protein Tn5397, CTn3-Orf14
18CD630_05060596830..598659 [+], 1830115249520  [UniProt]  [KEGG]Reverse transcriptase/maturase/endonuclease,Group II intron
19CD630_05070598817..599749 [+], 933115249521  [UniProt] putative conjugative transposon protein Tn5397,CTn3-Orf13
20CD630_05071599965..600018 [+], 54pseudogene Fragment of Tet(M) leader peptide Tn5397,CTn3-orf12 (C-terminal region)
21tetM600034..601953 [+], 1920115249523  [UniProt]  [KEGG]Tetracycline resistance protein Tn5397AR
22CD630_05081602049..602243 [+], 195328887544  [UniProt]  [KEGG]putative conjugative transposon protein Tn5397,CTn3-Orf6
23CD630_05090602302..602655 [-], 354115249525  [UniProt]  [KEGG]Transcriptional regulator, HTH-type Tn5397,CTn3-Orf9
24CD630_05091602866..602931 [+], 66115249526  [UniProt]  [KEGG]putative conjugative transposon protein Tn5397,CTn3-Orf10
25CD630_05100603157..603582 [+], 426115249527  [UniProt] putative RNA polymerase sigma factor Tn5397,CTn3-Orf7
26CD630_05101603579..603809 [+], 231115249528  [UniProt]  [KEGG]putative conjugative transposon protein Tn5397,CTn3-Orf8
27CD630_05102603931..604068 [-], 138pseudogene Fragment of putative conjugative transposon protein Tn5397, CTn3-Orf5 (C-terminal region)
28CD630_05103604190..604330 [+], 141328887545  [UniProt] putative conjugative transposon protein Tn5397,CTn3-Orf4
29tndX604427..606028 [+], 1602115249530  [UniProt] Recombinase site-specific resolvase family Tn5397, CTn3-Orf3
30CD630_04950584769..606563 [+], 21795pseudogene Fragment of putative regulatory protein
31CD630_05130606589..606777 [+], 189115249531 conserved hypothetical protein
flank Flanking regions
integrase Gene may contribute to site-specific recombination

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
(1) Jasni AS; Mullany P; Hussain H; Roberts AP (2010). Demonstration of conjugative transposon (Tn5397)-mediated horizontal gene transfer between Clostridium difficile and Enterococcus faecalis. Antimicrob Agents Chemother. 54(11):4924-6. [PudMed:20713671] experimental
(2) Sebaihia M; Wren BW; Mullany P; Fairweather NF; Minton N; Stabler R; Thomson NR; Roberts AP; Cerdeno-Tarraga AM; Wang H; Holden MT; Wright A; Churcher C; Quail MA; Baker S; Bason N; Brooks K; Chillingworth T; Cronin A; Davis P; Dowd L; Fraser A; Feltwell T; Hance Z; Holroyd S; Jagels K; Moule S; Mungall K; Price C; Rabbinowitsch E; Sharp S; Simmonds M; Stevens K; Unwin L; Whithead S; Dupuy B; Dougan G; Barrell B; Parkhill J (2006). The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome. Nat Genet. 38(7):779-86. [PudMed:16804543]
(3) Wang H; Smith MC; Mullany P (2006). The conjugative transposon Tn5397 has a strong preference for integration into its Clostridium difficile target site. J Bacteriol. 188(13):4871-8. [PudMed:16788196] experimental
(4) Roberts AP; Johanesen PA; Lyras D; Mullany P; Rood JI (2001). Comparison of Tn5397 from Clostridium difficile, Tn916 from Enterococcus faecalis and the CW459tet(M) element from Clostridium perfringens shows that they have similar conjugation regions but different insertion and excision modules. Microbiology. 147(Pt 5):1243-51. [PudMed:11320127] experimental
(5) Wang H; Mullany P (2000). The large resolvase TndX is required and sufficient for integration and excision of derivatives of the novel conjugative transposon Tn5397. J Bacteriol. 182(23):6577-83. [PudMed:11073898] experimental
(6) Wang H; Roberts AP; Lyras D; Rood JI; Wilks M; Mullany P (2000). Characterization of the ends and target sites of the novel conjugative transposon Tn5397 from Clostridium difficile: excision and circularization is mediated by the large resolvase, TndX. J Bacteriol. 182(13):3775-83. [PudMed:10850994] experimental
(7) Roberts AP; Pratten J; Wilson M; Mullany P (1999). Transfer of a conjugative transposon, Tn5397 in a model oral biofilm. FEMS Microbiol Lett. 177(1):63-6. [PudMed:10436923] experimental
(8) Mullany P; Pallen M; Wilks M; Stephen JR; Tabaqchali S (1996). A group II intron in a conjugative transposon from the gram-positive bacterium, Clostridium difficile. Gene. 174(1):145-50. [PudMed:8863741] experimental
(9) Mullany P; Wilks M; Tabaqchali S (1995). Transfer of macrolide-lincosamide-streptogramin B (MLS) resistance in Clostridium difficile is linked to a gene homologous with toxin A and is mediated by a conjugative transposon, Tn5398. J Antimicrob Chemother. 35(2):305-15. [PudMed:7759394] experimental
experimental experimental literature