ICEberg
ICEberg ID117
NamepSAM2
FamilyUnclassified
OrganismStreptomyces ambofaciens ATCC 15154 B3
Size (bp)10.9
GC content [Genome] (%)-
Insertion sitetRNA-Pro
FunctionChromosome mobilising ability
Species that ICE can be transferred toStreptomyces lividans
Nucleotide SequenceAF004315; Z19593; X14899; Z19594; Z19589; A09946; M22966; M22965; M22964; M22963; AJ005260 (partical ICE sequence)
experimental This is a ICE derived from experimental literature.
Incomplete gene list of pSAM2 from A09946
#GeneCoordinates [+/-], size (bp)Protein GI Product *
1xis88..291 [+], 204490194 excision protein
2int275..1441 [+], 1167490195 integrase
Incomplete gene list of pSAM2 from AJ005260
#GeneCoordinates [+/-], size (bp)Protein GI Product *
1orf131503..898 [-], 3963043515  [UniProt] Orf131
2korSA986..1765 [+], 7803043516  [UniProt] KorSA
3pra1905..2255 [+], 3513087885  [UniProt] Pra
4traSA2366..3613 [+], 12483043517  [UniProt] TraSA
5spdA3696..4370 [+], 6753043518  [UniProt] SpdA
6spdB4393..4569 [+], 1773043519  [UniProt] SpdB
7spdC4579..4734 [+], 1563043520  [UniProt] SpdC
8spdD4747..5061 [+], 3153043521  [UniProt] SpdD
9orf1835159..5710 [+], 5523043522  [UniProt] Orf183
10orf505804..5956 [-], 1533043523  [UniProt] -
11repSA5971..7350 [+], 13803087886  [UniProt] RepSA
12xis7347..7535 [+], 1893043524  [UniProt] Xis
13int7535..8701 [+], 11673043525  [UniProt] Int (integrase)
Incomplete gene list of pSAM2 from Z19594
#GeneCoordinates [+/-], size (bp)Protein GI Product *
1-154..705 [+], 552575387  [UniProt] Orf183
2-799..951 [-], 153575388  [UniProt] Orf50
3-966..2345 [+], 1380575389  [UniProt] RepSA
4-2342..2530 [+], 189298050  [UniProt] Xis
Incomplete gene list of pSAM2 from Z19593
#GeneCoordinates [+/-], size (bp)Protein GI Product *
1TraSA108..1355 [+], 12483123679  [UniProt] -
2SpdA1438..2112 [+], 675298054  [UniProt] -
3SpdB2135..2311 [+], 177298055  [UniProt] -
4SpdC2321..2476 [+], 156581574  [UniProt] -
5SpdD2489..2803 [+], 315298057  [UniProt] -
Incomplete gene list of pSAM2 from Z19589
#GeneCoordinates [+/-], size (bp)Protein GI Product *
1-266..1045 [+], 780298043  [UniProt] KorSA
Incomplete gene list of pSAM2 from X14899
#GeneCoordinates [+/-], size (bp)Protein GI Product *
1-159..347 [+], 18946649  [UniProt] -
2-347..1513 [+], 116746650  [UniProt] -
 
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer
regulation Gene may play role in regulation of transfer

ElementNo. of sequencesDownload
Nucleotide sequences11Fasta
Proteins27Fasta
(1) te Poele EM; Samborskyy M; Oliynyk M; Leadlay PF; Bolhuis H; Dijkhuizen L (2008). Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded. Plasmid. 59(3):202-16. [PudMed:18295883] in_silico
(2) Possoz C; Gagnat J; Sezonov G; Guerineau M; Pernodet JL (2003). Conjugal immunity of Streptomyces strains carrying the integrative element pSAM2 is due to the pif gene (pSAM2 immunity factor). Mol Microbiol. 47(5):1385-93. [PudMed:12603742] experimental
(3) Sezonov G; Duchene AM; Friedmann A; Guerineau M; Pernodet JL (1998). Replicase, excisionase, and integrase genes of the Streptomyces element pSAM2 constitute an operon positively regulated by the pra gene. J Bacteriol. 180(12):3056-61. [PudMed:9620953] experimental
(4) Seoane A; Navas J; Garcia Lobo JM (1997). Targets for pSAM2 integrase-mediated site-specific integration in the Mycobacterium smegmatis chromosome. Microbiology. 143 ( Pt 10):3375-80. [PudMed:9353939] experimental
(5) Sezonov G; Hagege J; Pernodet JL; Friedmann A; Guerineau M (1995). Characterization of pra, a gene for replication control in pSAM2, the integrating element of Streptomyces ambofaciens. Mol Microbiol. 17(3):533-44. [PudMed:8559072] experimental
(6) Hagege J; Pernodet JL; Sezonov G; Gerbaud C; Friedmann A; Guerineau M (1993). Transfer functions of the conjugative integrating element pSAM2 from Streptomyces ambofaciens: characterization of a kil-kor system associated with transfer. J Bacteriol. 175(17):5529-38. [PudMed:8366038] experimental
(7) Hagege J; Boccard F; Smokvina T; Pernodet JL; Friedmann A; Guerineau M (1994). Identification of a gene encoding the replication initiator protein of the Streptomyces integrating element, pSAM2. Plasmid. 31(2):166-83. [PudMed:8029324] experimental
(8) Hagege J; Pernodet JL; Friedmann A; Guerineau M (1993). Mode and origin of replication of pSAM2, a conjugative integrating element of Streptomyces ambofaciens. Mol Microbiol. 10(4):799-812. [PudMed:7934842] experimental
(9) Pernodet JL; Simonet JM; Guerineau M (1984). Plasmids in different strains of Streptomyces ambofaciens: free and integrated form of plasmid pSAM2. Mol Gen Genet. 198(1):35-41. [PudMed:6596483] experimental
(10) Boccard F; Smokvina T; Pernodet JL; Friedmann A; Guerineau M (1989). The integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens is related to temperate bacteriophages. EMBO J. 8(3):973-80. [PudMed:2721504] experimental
(11) Boccard F; Smokvina T; Pernodet JL; Friedmann A; Guerineau M (1989). Structural analysis of loci involved in pSAM2 site-specific integration in Streptomyces. Plasmid. 21(1):59-70. [PudMed:2657820] experimental
(12) Kuhstoss S; Richardson MA; Rao RN (1989). Site-specific integration in Streptomyces ambofaciens: localization of integration functions in S. ambofaciens plasmid pSAM2. J Bacteriol. 171(1):16-23. [PudMed:2536654] experimental
 
experimental experimental literature
in_silico in silico analysis literature