ICEberg
ICEberg ID122
NamepMEA100
FamilyUnclassified
OrganismAmycolatopsis mediterranei LBG A3136
Size (bp)23290
GC content [Genome] (%)-
Insertion sitetRNA-Phe
FunctionEliciting the lethal zygosis phenotype
Species that ICE can be transferred to-
Nucleotide SequenceEU149765 (complete ICE sequence in this GenBank file)
Replicon-
Coordinates1..23290
Link to view genome context of this ICE in the genome browser
experimental This is a ICE derived from experimental literature.
Complete gene list of pMEA100 form EU149765
#GeneCoordinates [+/-], size (bp)Protein GI Product *
1repAM1..1113 [+], 1113161087188  [UniProt] replication initiator protein
2xis1434..1736 [+], 303161087189  [UniProt] excisionase
3int1752..3098 [+], 1347161087190  [UniProt] bacteriophage-related integrase
4amp1043394..3855 [+], 462161087191  [UniProt] hypothetical protein
5amp1054891..5238 [+], 348161087192  [UniProt] hypothetical protein
6amp1065552..6346 [-], 795161087193  [UniProt] hypothetical protein
7mdp7116..7688 [+], 573161087194  [UniProt] metal dependent phosphohydrolase
8nud17680..8156 [-], 477161087195  [UniProt] NUDIX hydrolase
9amp1098162..8911 [-], 750161087196  [UniProt] XRE family transcriptional regulator
10amp1109084..9287 [+], 204161087197  [UniProt] pMEA-specific protein
11korA9284..9631 [-], 348161087198  [UniProt] GntR family transcriptional regulator
12amp1129752..10009 [+], 258161087199  [UniProt] hypothetical protein
13amp11310318..11040 [+], 723161087200  [UniProt] hypothetical protein
14nud211081..11605 [+], 525161087201  [UniProt] NUDIX hydrolase
15ssgA11623..11976 [+], 354161087202  [UniProt] SsgA family transcriptional regulator
16traF12445..12675 [+], 231161087203  [UniProt] TraF
17traG12675..12989 [+], 315161087204  [UniProt] TraG
18traH12880..13314 [+], 435161087205  [UniProt] TraH
19traI13311..14492 [+], 1182161087206  [UniProt] TraI
20traJ14599..16911 [+], 2313161087207  [UniProt] TraJ
21amp12117208..17654 [+], 447161087208  [UniProt] hypothetical protein
22amp12217644..18387 [-], 744161087209  [UniProt] hypothetical protein
23ssb18442..18924 [+], 483161087210  [UniProt] single-strand DNA-binding protein
24amp12419233..20189 [+], 957161087211  [UniProt] N-terminal primase-polymerase (primpol)-domain containing protein
25amp12520523..21023 [+], 501161087212  [UniProt] hypothetical protein
26amp12621020..21373 [+], 354161087213  [UniProt] hypothetical protein
27dmt21373..22998 [+], 1626161087214  [UniProt] C-5 cytosine-specific DNA methylase
 
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins27Fasta
(1) te Poele EM; Samborskyy M; Oliynyk M; Leadlay PF; Bolhuis H; Dijkhuizen L (2008). Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded. Plasmid. 59(3):202-16. [PudMed:18295883] in_silico
(2) te Poele EM; Habets MN; Tan GY; Ward AC; Goodfellow M; Bolhuis H; Dijkhuizen L (2007). Prevalence and distribution of nucleotide sequences typical for pMEA-like accessory genetic elements in the genus Amycolatopsis. FEMS Microbiol Ecol. 61(2):285-94. [PudMed:17535299] experimental
(3) Moretti P; Hintermann G; Hutter R (1985). Isolation and characterization of an extrachromosomal element from Nocardia mediterranei. Plasmid. 14(2):126-33. [PudMed:2999850] experimental
(4) Madon J; Moretti P; Hutter R (1987). Site-specific integration and excision of pMEA100 in Nocardia mediterranei. Mol Gen Genet. 209(2):257-64. [PudMed:2823074] experimental
 
experimental experimental literature
in_silico in silico analysis literature