ICEberg ID306
OrganismEnterococcus faecalis plasmid pCF10
Size (bp)18032
GC content [Genome] (%)38.7 [36]
Insertion siteAT rich regions
Species that ICE can be transferred to-
Nucleotide SequenceAY855841 (complete ICE sequence in this genome)
Replicon plasmid pCF10 (Taxonomy ID: 1351) [NC_006827]
Genome coordinates40068..58099
Link to view genome context of this ICE in the genome browser
experimental This is a ICE derived from experimental literature.
Complete gene list of Tn925 form AY855841
#GeneCoordinates [+/-], size (bp)Protein GI Product *
1pcfI35680..35847 [+], 16857489120 PcfI
2pcfJ35873..37210 [-], 133857489121 PcfJ
3pcfK37207..37983 [-], 77757489122 PcfK
4pcfL38126..38674 [-], 54957489123 PcfL
5pcfM38698..38889 [-], 19257489141 PcfM
6pcfN38874..39059 [-], 18657489124 PcfN
7pcfO39065..39514 [-], 45057489125 PcfO
8pcfP39801..40001 [+], 20157489126 PcfP
9pcfQ58142..58372 [-], 23157489127 PcfQ
10pcfR58427..58921 [+], 49557489128 PcfR
11pcfS58987..59439 [+], 45357489129 PcfS
12pcfT59617..60195 [+], 57957489130 PcfT
13pcfU60277..60606 [+], 33057489131 PcfU
14pcfV60829..61854 [+], 102657489132 PcfV
15pcfW62776..63048 [+], 27357489133 PcfW
flank Flanking regions

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
(1) Hirt H; Manias DA; Bryan EM; Klein JR; Marklund JK; Staddon JH; Paustian ML; Kapur V; Dunny GM (2005). Characterization of the pheromone response of the Enterococcus faecalis conjugative plasmid pCF10: complete sequence and comparative analysis of the transcriptional and phenotypic responses of pCF10-containing cells to pheromone induction. J Bacteriol. 187(3):1044-54. [PudMed:15659682] experimental
(2) Hedberg PJ; Leonard BA; Ruhfel RE; Dunny GM (1996). Identification and characterization of the genes of Enterococcus faecalis plasmid pCF10 involved in replication and in negative control of pheromone-inducible conjugation. Plasmid. 35(1):46-57. [PudMed:8693026] experimental
(3) Ruhfel RE; Manias DA; Dunny GM (1993). Cloning and characterization of a region of the Enterococcus faecalis conjugative plasmid, pCF10, encoding a sex pheromone-binding function. J Bacteriol. 175(16):5253-9. [PudMed:8349565] experimental
(4) Nakayama J; Ruhfel RE; Dunny GM; Isogai A; Suzuki A (1994). The prgQ gene of the Enterococcus faecalis tetracycline resistance plasmid pCF10 encodes a peptide inhibitor, iCF10. J Bacteriol. 176(23):7405-8. [PudMed:7545961] experimental
(5) Christie PJ; Korman RZ; Zahler SA; Adsit JC; Dunny GM (1987). Two conjugation systems associated with Streptococcus faecalis plasmid pCF10: identification of a conjugative transposon that transfers between S. faecalis and Bacillus subtilis. J Bacteriol. 169(6):2529-36. [PudMed:3034859] experimental
(6) Kao SM; Olmsted SB; Viksnins AS; Gallo JC; Dunny GM (1991). Molecular and genetic analysis of a region of plasmid pCF10 containing positive control genes and structural genes encoding surface proteins involved in pheromone-inducible conjugation in Enterococcus faecalis. J Bacteriol. 173(23):7650-64. [PudMed:1938961] experimental
(7) Torres OR; Korman RZ; Zahler SA; Dunny GM (1991). The conjugative transposon Tn925: enhancement of conjugal transfer by tetracycline in Enterococcus faecalis and mobilization of chromosomal genes in Bacillus subtilis and E. faecalis. Mol Gen Genet. 225(3):395-400. [PudMed:1850085] experimental
(8) Guffanti AA; Quirk PG; Krulwich TA (1991). Transfer of Tn925 and plasmids between Bacillus subtilis and alkaliphilic Bacillus firmus OF4 during Tn925-mediated conjugation. J Bacteriol. 173(5):1686-9. [PudMed:1847906] experimental
experimental experimental literature