![]() | 44 |
![]() | SXT(MO10) |
![]() | SXT/R391 |
![]() | Vibrio cholerae O139 MO10 |
![]() | 99483 |
![]() | 47 [47] |
![]() | prfC |
![]() | Resistance to the antibiotics sulfamethoxazole, trimethoprim, chloramphenicol, and streptomycin; Toxin–Antitoxin System |
![]() | Vibrio cholerae; Escherichia coli; Salmonella enterica serovar Typhimurium |
![]() | AY055428 (complete ICE sequence in this GenBank file) |
![]() | - |
![]() | 1..99483 |
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Complete gene list of SXT(MO10) form AY055428 | |||||
# | Gene | Coordinates [+/-], size (bp) | Protein GI | Product | * |
1 | int | 833..2074 [-], 1242 | 21885342 [UniProt] | integrase | |
2 | s002 | 2076..2345 [-], 270 | 21885339 [UniProt] | unknown | |
3 | s003 | 2348..3322 [-], 975 | 21885338 [UniProt] | unknown | |
4 | rumB' | 4241..5413 [-], 1173 | 21885341 [UniProt] | UV repair DNA polymerase | |
5 | tnp | 5697..6290 [+], 594 | 21885282 [UniProt] | putative transposase | |
6 | tnpA | 6404..9304 [+], 2901 | 21885281 [UniProt] | putative transposase | |
7 | tnpB | 9799..11292 [+], 1494 | 21885280 [UniProt] | putative transposase | |
8 | dhfR18 | 11653..12207 [-], 555 | 21885345 [UniProt] | dhfR18 | |
9 | s009 | 12221..12766 [-], 546 | 21885337 [UniProt] | unknown | |
10 | s010 | 12766..13932 [-], 1167 | 21885336 [UniProt] | unknown | |
11 | dcd | 13947..14675 [-], 729 | 21885344 [UniProt] | deoxycytidine triphosphate deaminase | |
12 | 'tnpB | 14935..15267 [+], 333 | 21885346 [UniProt] | putative transposase | |
13 | s013 | 15298..16182 [+], 885 | 21885335 [UniProt] | unknown | AR |
14 | floR | 16399..17613 [+], 1215 | 21885343 [UniProt] | florfenicol exporter | AR |
15 | s015 | 17641..17946 [+], 306 | 21885334 [UniProt] | unknown | |
16 | tnpB' | 18058..18597 [+], 540 | 21885279 [UniProt] | putative transposase | |
17 | strB | 18569..19405 [-], 837 | 21885283 [UniProt] | streptomycin phosphotransferase | AR |
18 | strA | 19405..20208 [-], 804 | 21885284 [UniProt] | streptomycin phosphotransferase | AR |
19 | sulII | 20269..21084 [-], 816 | 21885347 [UniProt] | dihydropteroate synthase type II | AR |
20 | tnpA' | 21555..23168 [+], 1614 | 21885278 [UniProt] | putative transposase | |
21 | s021 | 22636..23358 [-], 723 | 21885333 [UniProt] | unknown | |
22 | 'rumB | 23511..23831 [-], 321 | 21885348 [UniProt] | UV repair DNA polymerase | |
23 | rumA | 23788..24237 [-], 450 | 21885340 [UniProt] | UV repair | |
24 | s024 | 24876..25784 [+], 909 | 21885332 [UniProt] | unknown | |
25 | s025 | 25858..26172 [+], 315 | 21885331 [UniProt] | unknown | |
26 | s026 | 26241..27167 [+], 927 | 21885330 [UniProt] | unknown | |
27 | s027 | 27395..27778 [+], 384 | 21885329 [UniProt] | unknown | |
28 | s028 | 27766..28359 [+], 594 | 21885328 [UniProt] | unknown | |
29 | s029 | 28378..30273 [+], 1896 | 21885327 [UniProt] | unknown | |
30 | s030 | 30325..31923 [+], 1599 | 21885326 [UniProt] | unknown | |
31 | s031 | 32037..32519 [+], 483 | 21885325 [UniProt] | unknown | |
32 | s032 | 32488..33240 [+], 753 | 21885324 [UniProt] | unknown | |
33 | s033 | 33237..33995 [+], 759 | 21885323 [UniProt] | unknown | |
34 | s034 | 33999..34679 [+], 681 | 21885322 [UniProt] | unknown | |
35 | s035 | 34672..35871 [+], 1200 | 21885321 [UniProt] | unknown | |
36 | s036 | 35852..36232 [+], 381 | 21885320 [UniProt] | unknown | |
37 | s037 | 36399..37013 [+], 615 | 21885319 [UniProt] | unknown | |
38 | s038 | 37601..40234 [+], 2634 | 21885318 [UniProt] | unknown | |
39 | s039 | 40251..42326 [+], 2076 | 21885317 [UniProt] | unknown | |
40 | s040 | 42331..45021 [+], 2691 | 21885316 [UniProt] | unknown | |
41 | traI | 45176..47326 [+], 2151 | 21885269 [UniProt] | conjugative relaxase | |
42 | traD | 47396..49195 [+], 1800 | 21885274 [UniProt] | conjugative coupling factor | |
43 | s043 | 49753..50388 [+], 636 | 21885315 [UniProt] | putative conjugation coupling factor | |
44 | s044 | 50414..50692 [-], 279 | 21885314 [UniProt] | unknown | |
45 | s045 | 50689..51012 [-], 324 | 21885313 [UniProt] | unknown | |
46 | traL | 51187..51474 [+], 288 | 21885268 [UniProt] | sex pilus assembly | |
47 | traE | 51471..52097 [+], 627 | 21885273 [UniProt] | sex pilus assembly | |
48 | s048 | 52078..52977 [+], 900 | 21885312 [UniProt] | unknown | |
49 | traB | 52980..54269 [+], 1290 | 21885276 [UniProt] | sex pilus assembly | |
50 | traV | 54266..54916 [+], 651 | 21885266 [UniProt] | sex pilus assembly | |
51 | traA | 54913..55299 [+], 387 | 21885277 [UniProt] | putative pilin subunit | |
52 | s052 | 55664..56311 [+], 648 | 21885311 [UniProt] | unknown | TA |
53 | s053 | 56292..57242 [+], 951 | 21885310 [UniProt] | unknown | TA |
54 | s054 | 57374..58066 [+], 693 | 21885309 [UniProt] | putative disulfide bond isomerase | |
55 | traC | 58066..60465 [+], 2400 | 21885275 [UniProt] | sex pilus assembly | |
56 | trsF | 60789..61301 [+], 513 | 21885264 [UniProt] | conjugation signal peptidase | |
57 | traW | 61216..62436 [+], 1221 | 21885265 [UniProt] | sex pilus assembly | |
58 | traU | 62420..63448 [+], 1029 | 21885267 [UniProt] | sex pilus assembly | |
59 | traN | 63683..67147 [+], 3465 | 21885349 [UniProt] | mating pair stabilization | |
60 | s060 | 67924..69006 [-], 1083 | 21885308 [UniProt] | unknown | |
61 | s061 | 69006..70070 [-], 1065 | 21885307 [UniProt] | unknown | |
62 | s062 | 70183..70899 [-], 717 | 21885306 [UniProt] | unknown | |
63 | s063 | 71008..71610 [-], 603 | 21885305 [UniProt] | unknown | |
64 | ssb | 72318..72737 [+], 420 | 21885285 [UniProt] | single-stranded DNA binding protein | VF |
65 | s065 | 72817..73635 [+], 819 | 21885304 [UniProt] | putative DNA recombination protein | |
66 | s066 | 73921..74937 [+], 1017 | 21885303 [UniProt] | unknown | |
67 | s067 | 75030..76106 [+], 1077 | 21885302 [UniProt] | unknown | |
68 | s068 | 76975..77928 [+], 954 | 21885301 [UniProt] | unknown | |
69 | s069 | 77990..78430 [+], 441 | 21885300 [UniProt] | unknown | |
70 | s070 | 78500..80155 [+], 1656 | 21885299 [UniProt] | unknown | |
71 | s071 | 80237..80734 [+], 498 | 21885298 [UniProt] | putative DNA repair radC | VF |
72 | s072 | 81243..82244 [+], 1002 | 21885297 [UniProt] | unknown | |
73 | s073 | 82335..83042 [+], 708 | 21885296 [UniProt] | unknown | |
74 | - | 83304..84233 [-], 930 | 21885295 [UniProt] | putative response regulator | |
75 | - | 84239..88045 [-], 3807 | 21885294 [UniProt] | putative histidine kinase | VF |
76 | traF | 88747..89724 [+], 978 | 21885272 [UniProt] | sex pilus assembly | |
77 | traH | 89727..91115 [+], 1389 | 21885270 [UniProt] | sex pilus assembly | |
78 | traG | 91119..94688 [+], 3570 | 21885271 [UniProt] | sex pilus assembly | |
79 | s079 | 94721..95173 [-], 453 | 21885293 [UniProt] | unknown | |
80 | setC | 95207..95740 [-], 534 | 21885287 [UniProt] | transcriptional activator | |
81 | setD | 95737..96036 [-], 300 | 21885350 [UniProt] | transcriptional activator | |
82 | s082 | 96033..96581 [-], 549 | 21885292 [UniProt] | unknown | |
83 | s083 | 96568..96936 [-], 369 | 21885291 [UniProt] | unknown | |
84 | s084 | 96926..97231 [-], 306 | 21885290 [UniProt] | unknown | |
85 | s085 | 97218..97739 [-], 522 | 21885289 [UniProt] | unknown | |
86 | s086 | 98144..98395 [-], 252 | 21885288 [UniProt] | unknown | |
87 | setR | 98513..99160 [+], 648 | 21885286 [UniProt] | transcriptional repressor |
(1) Daccord A; Mursell M; Poulin-Laprade D; Burrus V (2012). Dynamics of the SetCD-Regulated Integration and Excision of Genomic Islands Mobilized by Integrating Conjugative Elements of the SXT/R391 Family. J Bacteriol. 194(21):5794-802. [PudMed:22923590] ![]() |
(2) Pande K; Mendiratta DK; Vijayashri D; Thamke DC; Narang P (2012). SXT constin among Vibrio cholerae isolates from a tertiary care hospital. Indian J Med Res. 135:346-50. [PudMed:22561621] ![]() |
(3) Spagnoletti M; Ceccarelli D; Colombo MM (2012). Rapid detection by multiplex PCR of Genomic Islands, prophages and Integrative Conjugative Elements in V. cholerae 7th pandemic variants. J Microbiol Methods. 88(1):98-102. [PudMed:22062086] ![]() |
(4) Chen WY; Ho JW; Huang JD; Watt RM (2011). Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae. BMC Mol Biol. 12(1):16. [PudMed:21501469] ![]() |
(5) Wozniak RA; Fouts DE; Spagnoletti M; Colombo MM; Ceccarelli D; Garriss G; Dery C; Burrus V; Waldor MK (2009). Comparative ICE genomics: insights into the evolution of the SXT/R391 family of ICEs. PLoS Genet. 5(12):e1000786. [PudMed:20041216] ![]() |
(6) Bordeleau E; Brouillette E; Robichaud N; Burrus V (2010). Beyond antibiotic resistance: integrating conjugative elements of the SXT/R391 family that encode novel diguanylate cyclases participate to c-di-GMP signalling in Vibrio cholerae. Environ Microbiol. 12(2):510-23. [PudMed:19888998] ![]() |
(7) Wozniak RA; Waldor MK (2009). A toxin-antitoxin system promotes the maintenance of an integrative conjugative element. PLoS Genet. 5(3):e1000439. [PudMed:19325886] ![]() |
(8) Ceccarelli D; Daccord A; Rene M; Burrus V (2008). Identification of the origin of transfer (oriT) and a new gene required for mobilization of the SXT/R391 family of integrating conjugative elements. J Bacteriol. 190(15):5328-38. [PudMed:18539733] ![]() |
(9) Marrero J; Waldor MK (2007). The SXT/R391 family of integrative conjugative elements is composed of two exclusion groups. J Bacteriol. 189(8):3302-5. [PudMed:17307849] ![]() |
(10) McLeod SM; Burrus V; Waldor MK (2006). Requirement for Vibrio cholerae integration host factor in conjugative DNA transfer. J Bacteriol. 188(16):5704-11. [PudMed:16885438] ![]() |
(11) Marrero J; Waldor MK (2005). Interactions between inner membrane proteins in donor and recipient cells limit conjugal DNA transfer. Dev Cell. 8(6):963-70. [PudMed:15935784] ![]() |
(12) Beaber JW; Waldor MK (2004). Identification of operators and promoters that control SXT conjugative transfer. J Bacteriol. 186(17):5945-9. [PudMed:15317801] ![]() |
(13) Burrus V; Waldor MK (2004). Formation of SXT tandem arrays and SXT-R391 hybrids. J Bacteriol. 186(9):2636-45. [PudMed:15090504] ![]() |
(14) Boltner D; Osborn AM (2004). Structural comparison of the integrative and conjugative elements R391, pMERPH, R997, and SXT. Plasmid. 51(1):12-23. [PudMed:14711525] ![]() |
(15) Beaber JW; Hochhut B; Waldor MK (2004). SOS response promotes horizontal dissemination of antibiotic resistance genes. Nature. 427(6969):72-4. [PudMed:14688795] ![]() |
(16) Burrus V; Waldor MK (2003). Control of SXT integration and excision. J Bacteriol. 185(17):5045-54. [PudMed:12923077] ![]() |
(17) Thungapathra M; Amita; Sinha KK; Chaudhuri SR; Garg P; Ramamurthy T; Nair GB; Ghosh A (2002). Occurrence of antibiotic resistance gene cassettes aac(6')-Ib, dfrA5, dfrA12, and ereA2 in class I integrons in non-O1, non-O139 Vibrio cholerae strains in India. Antimicrob Agents Chemother. 46(9):2948-55. [PudMed:12183252] ![]() |
(18) Beaber JW; Hochhut B; Waldor MK (2002). Genomic and functional analyses of SXT, an integrating antibiotic resistance gene transfer element derived from Vibrio cholerae. J Bacteriol. 184(15):4259-69. [PudMed:12107144] ![]() |
(19) Hochhut B; Lotfi Y; Mazel D; Faruque SM; Woodgate R; Waldor MK (2001). Molecular analysis of antibiotic resistance gene clusters in vibrio cholerae O139 and O1 SXT constins. Antimicrob Agents Chemother. 45(11):2991-3000. [PudMed:11600347] ![]() |
(20) Hochhut B; Beaber JW; Woodgate R; Waldor MK (2001). Formation of chromosomal tandem arrays of the SXT element and R391, two conjugative chromosomally integrating elements that share an attachment site. J Bacteriol. 183(4):1124-32. [PudMed:11157923] ![]() |
(21) Hochhut B; Marrero J; Waldor MK (2000). Mobilization of plasmids and chromosomal DNA mediated by the SXT element, a constin found in Vibrio cholerae O139. J Bacteriol. 182(7):2043-7. [PudMed:10715015] ![]() |
(22) Hochhut B; Waldor MK (1999). Site-specific integration of the conjugal Vibrio cholerae SXT element into prfC. Mol Microbiol. 32(1):99-110. [PudMed:10216863] ![]() |
(23) Waldor MK; Tschape H; Mekalanos JJ (1996). A new type of conjugative transposon encodes resistance to sulfamethoxazole, trimethoprim, and streptomycin in Vibrio cholerae O139. J Bacteriol. 178(14):4157-65. [PudMed:8763944] ![]() |
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