SecReT4
Protein ID: 145301426
T4SS name [ID]Tra_F [130]
Gene symboltraI
Locus tagASA_P5G043
StrainAeromonas salmonicida subsp. salmonicida A449
Repliconplasmid 5 [GenBank: NC_009350] [Browse all T4SS(s) in this replicon]
Location [Strand]42906..48068 [+]
Productconjugal transfer nickase/helicase TraI
Component typeTraI_F
UniProt IDA4SUE5
KEGG IDasa:ASA_P5G043
PDB IDNA
Domain hit(s) vs Pfam TrwC [PF08751], Evalue: 2.9e-77, Aligned region: 5..296
TraI [PF07057], Evalue: 2.5e-51, Aligned region: 1408..1535
AAA_30 [PF13604], Evalue: 1.1e-42, Aligned region: 962..1146
AAA_19 [PF13245], Evalue: 5.3e-05, Aligned region: 978..1040
Protein Sequence: 1720 a.a.     [Download]
>gi|145301426|ref|YP_001144265.1| TraI protein [Aeromonas salmonicida subsp. salmonicida A449]
MLSLSTIKGSAAYYAAEENYYAMGELESVWMGEGADRLGLSGPIDGADRLGLSGPIDGATLDAIAQGKLP
DGTELSRIVDGKQTHRTGYDLTFSAPKSVSVMALVAGDERFLAAHHHAVAVAMKEVEALASTRIMTDGVS
RTETTGNIVAGLYTHDTSRELDPQLHTHSLVLNATWAEGKWRALASDTKTNHGFYEAMLANQIAIGKVYR
HALRQDIEAMGFETEVTGKHGMWELKGVPTEPFSQRSQQINEAVGPDASPKSRDVAALDTRKDKAVADPD
LLIADWRDRLGKAGFELPLYLAKADERAQGYTVPTPAGTDITQAVSQAISILSDKKVQFSYSELLAATVS
QLPAEQGLITQAREGIEQAIGRERLIPLDKEKGIFTSDIHLLNELSIAQLANEFNKRPPIATFAERATPR
DRPYADAYSVLAQDKAPLAILSGRGGMAQTIERMEDAVLMASEQGREVTILASDNRSARLLAQSQPLAGN
IAPRHTLNAATVFAPHSTLIVEQAESLTLKETLLILEKARASDVQVLMLDSERQRGVGNALSVLKQASVP
QYRFYDAPNIEATVQSVPDKPARFDALAQDYAHRLQQGEQVVAQVSGTRDQQQLTSYIRSVLRDSGQLHG
QDHTLNVLAPVWLDSKTRHQRDTYREGMVMERWDDKGKTMDRYRIDLVADKTHSLVLVSGQGEKHTIKIR
ALDSNWSLFESKRIEVAAGDTLRVLGREAEGTLKAQQTLQVLDAAPGHLHLKTPTGELQLPTDRALKLDH
NYVTGVGVTASQEATVLAAMSARQMSGTGLNQLARSGQHIHIYTPLDQMRAQSKLASHPQFQLASEKLKA
HGDSDQLDTTLKQATASLHTAAEQALHLGLAQVEAKGIVFSTSSLLAAALDFAPATPIDSISAVLDDKLA
KGDLLPVPGKRGDVVSRASFDMEKFIIATIAQGKGTATPYLQQVPDPQLAGLTPGQAQSTRLILESNDQF
IAIQGYAGVGKTTQFKAVLSALDTLPPKQRPTVVGIGPTHRAVHEMQSTGVKAQTLASFLSESRLQAMAG
EQQDHSRTLFLVDESSMIGNRDMAEFYQRVAASGARVVSSGDTAQLKAISSGAPFQLMQERSAVDVAVMK
EIVRQRPELKPAIYSLIDGNLTQSLAQREAVQPGVVPRDPANEPDAWTPDRSVIEAKNPAELVVKDYASR
TRLTRDNTMVIVHLNEVRHGINAAIHQALFDKGELGPRQTSLTILDPVRIEENSLRSSEQVQKNALLGKV
AVLDQHYYTISGIDPQAGTMTIKDNEGKVRLISNIENTAHDLAIYQPREIKVSEGDKIRFTRTVNEHGHV
ANSQWQVKRIEDNGAIVLHDGNNEKTVRPDQFKEDQHIDLGYAITAYGAQGASSTYAIELQAIKGVNRNL
ITLSSAYVPASRAKEHMQTYTDDKEGWINLLNKNLKSQVSTAHDALEGDQIKGLKVADMLLGAASPMRST
ALGRHVLKTHQLDEQQHMGKYIAPGKKYPTPHAAFPVWDNNGKPAGAAMVELQSHHQNKGPTLDSEYRLV
ANERAEFIGLQRASNGETRIATSLEDGIAMATKHPHSGILVSINGAPPPFNVNRMTGGKLVVSDTDAQHL
QKESERELPLPQDMTTKEAKRQDEMIKTVAREKEQPDIKLPDDKQQAVNRDDKRLHNDIHQLNKDAELSE
RQLRKLAEIRDVAKAKFTHELHDKLDKLEREIIKELTKGE
 
Nucleotide Sequence: 5163 bp     [Download]
>gi|145301385|ref|NC_009350.1|:42906-48068 Aeromonas salmonicida subsp. salmonicida A449 plasmid 5, complete sequence
ATGCTGTCACTTTCCACCATCAAGGGCAGCGCGGCCTACTACGCTGCCGAGGAAAACTATTACGCCATGG
GCGAGCTCGAATCGGTCTGGATGGGGGAAGGGGCCGACCGCCTTGGACTGAGTGGCCCCATTGACGGGGC
CGACCGCCTTGGACTGAGTGGCCCCATTGACGGGGCCACCCTCGATGCCATTGCCCAGGGTAAACTCCCC
GATGGCACTGAACTCTCGCGCATCGTGGATGGCAAGCAGACTCACCGCACCGGCTATGACCTGACGTTTT
CGGCCCCCAAGAGTGTCTCCGTGATGGCACTGGTGGCGGGTGACGAGCGCTTTCTGGCCGCCCACCATCA
CGCTGTGGCGGTGGCAATGAAGGAGGTCGAGGCGCTGGCCAGCACCCGCATCATGACCGATGGGGTATCG
CGCACCGAGACCACCGGCAACATCGTGGCGGGCCTTTACACCCACGACACCTCCCGCGAACTCGATCCCC
AGCTTCACACCCACTCGCTAGTGCTTAACGCCACCTGGGCCGAGGGCAAATGGCGGGCGCTGGCCAGTGA
CACCAAGACCAACCACGGTTTTTATGAAGCCATGCTCGCCAACCAGATAGCCATAGGCAAGGTGTACCGC
CACGCCCTGCGCCAGGACATCGAGGCGATGGGCTTTGAAACCGAAGTCACCGGCAAACATGGTATGTGGG
AACTCAAGGGAGTACCGACAGAACCATTCAGCCAGCGCAGTCAGCAAATCAACGAGGCTGTAGGCCCCGA
TGCCTCACCAAAATCCCGCGATGTCGCAGCGCTCGATACCCGCAAGGACAAGGCGGTTGCCGACCCCGAT
CTACTGATCGCCGATTGGCGTGATCGCCTGGGCAAAGCAGGCTTTGAACTGCCCCTCTATCTCGCCAAGG
CCGACGAACGTGCCCAGGGATACACCGTCCCCACGCCAGCTGGCACGGATATCACCCAAGCCGTGAGCCA
GGCGATATCCATACTTTCGGATAAAAAGGTACAGTTCTCCTACTCCGAATTACTGGCCGCCACGGTGAGC
CAGCTCCCTGCCGAACAGGGGTTGATCACCCAGGCCCGCGAGGGTATCGAGCAGGCGATTGGGCGCGAGC
GCCTCATCCCGCTCGACAAGGAAAAAGGGATCTTCACCTCCGATATCCATCTGCTCAACGAATTGTCCAT
CGCCCAGCTGGCCAACGAGTTCAACAAGCGCCCCCCGATAGCCACCTTTGCCGAGCGGGCAACCCCGCGT
GATCGCCCCTATGCCGACGCCTACTCTGTGTTGGCCCAGGACAAGGCCCCGCTGGCTATCCTGTCCGGGC
GGGGTGGCATGGCCCAAACCATCGAGCGCATGGAAGATGCCGTGCTCATGGCCAGCGAACAGGGGCGTGA
GGTCACCATCCTGGCCAGCGACAATCGCTCGGCTCGCCTGCTGGCACAAAGCCAGCCTCTGGCAGGCAAT
ATTGCCCCGCGTCACACCCTCAATGCCGCCACGGTCTTTGCCCCTCATAGCACCCTCATTGTCGAGCAGG
CTGAATCCCTGACCCTCAAAGAAACGCTGCTCATCCTGGAAAAGGCACGGGCCAGCGATGTCCAGGTACT
GATGCTCGACAGTGAGCGCCAGCGCGGCGTCGGCAATGCGCTCTCCGTCCTCAAGCAAGCCAGCGTGCCG
CAGTACCGTTTCTATGATGCGCCCAACATCGAGGCCACCGTCCAAAGCGTGCCTGACAAGCCAGCGCGCT
TTGACGCCCTGGCACAGGACTACGCGCATCGTCTGCAGCAAGGCGAGCAGGTGGTTGCCCAGGTCTCCGG
GACACGGGATCAGCAGCAGCTAACCAGCTATATCCGCAGCGTCTTGCGGGATAGCGGCCAACTGCACGGG
CAGGATCACACCCTCAACGTCCTTGCCCCGGTATGGCTCGATAGCAAGACCCGCCATCAGCGTGACACCT
ATCGCGAGGGGATGGTCATGGAGCGGTGGGACGACAAGGGTAAAACCATGGATCGCTATCGCATTGATCT
CGTCGCAGACAAGACCCACTCGCTGGTGCTGGTAAGCGGGCAGGGGGAGAAACACACCATCAAGATCCGT
GCCCTGGACAGCAACTGGTCGCTGTTTGAAAGCAAGCGCATCGAGGTCGCGGCGGGCGACACACTCCGGG
TGCTCGGGCGAGAGGCCGAGGGTACCCTCAAGGCGCAACAGACCCTGCAGGTACTGGACGCGGCCCCTGG
CCACCTGCACCTCAAGACCCCGACCGGTGAGCTGCAACTGCCAACAGACAGGGCACTCAAGCTCGACCAC
AACTATGTTACCGGCGTGGGCGTCACCGCCAGCCAGGAGGCCACCGTACTGGCCGCCATGAGTGCCCGCC
AGATGAGTGGCACAGGGCTTAACCAACTGGCCAGAAGTGGCCAGCATATTCACATCTACACCCCGCTTGA
TCAGATGCGGGCACAGAGCAAGTTAGCCAGTCATCCCCAGTTCCAGCTGGCCAGTGAAAAGCTCAAAGCC
CACGGCGACAGCGACCAGCTCGATACTACGCTCAAGCAGGCCACCGCCAGCCTGCACACAGCGGCTGAGC
AAGCCCTGCATTTGGGGCTGGCCCAGGTGGAAGCCAAAGGCATCGTCTTTTCCACCTCAAGCTTGCTGGC
CGCCGCCCTGGATTTTGCCCCGGCAACCCCGATTGACTCAATCAGCGCCGTGCTCGATGACAAACTCGCC
AAGGGTGACCTGCTGCCGGTGCCCGGCAAGCGCGGCGACGTGGTCTCTCGAGCCAGCTTTGACATGGAAA
AGTTCATTATCGCCACCATTGCCCAAGGCAAGGGAACCGCGACCCCCTACCTGCAGCAGGTACCCGACCC
CCAACTGGCAGGGCTAACCCCTGGCCAGGCACAATCCACTCGCCTGATCCTGGAGAGCAACGACCAGTTC
ATCGCCATCCAGGGCTATGCCGGTGTGGGTAAAACCACCCAGTTCAAGGCCGTGCTGAGTGCACTCGACA
CCCTGCCCCCGAAACAGCGCCCGACCGTGGTCGGGATCGGCCCCACCCACCGGGCCGTCCATGAAATGCA
GTCGACCGGGGTCAAGGCACAGACGCTGGCCTCGTTTCTCTCGGAGAGTCGCCTGCAGGCCATGGCAGGG
GAGCAGCAGGACCACAGCCGCACCCTGTTCCTGGTGGATGAAAGCTCGATGATTGGCAACCGCGACATGG
CGGAGTTTTACCAGCGGGTGGCCGCCAGTGGCGCAAGAGTGGTCAGCAGCGGTGATACCGCGCAGCTCAA
AGCCATCTCGTCAGGTGCCCCGTTCCAACTGATGCAAGAGCGCAGCGCGGTCGATGTGGCTGTCATGAAA
GAGATTGTCCGCCAGCGCCCGGAGCTCAAACCGGCGATCTACAGCTTGATAGATGGCAACCTCACCCAGT
CACTGGCACAGCGCGAAGCGGTGCAACCGGGTGTGGTACCCCGAGACCCGGCAAACGAACCGGATGCCTG
GACACCGGATCGATCGGTGATTGAGGCAAAGAACCCCGCCGAACTGGTGGTCAAGGATTATGCCAGCCGT
ACCCGCCTGACACGGGATAACACCATGGTGATCGTGCATCTCAATGAGGTGCGCCACGGTATTAACGCCG
CAATACACCAGGCACTGTTTGATAAAGGGGAATTAGGCCCACGCCAGACCAGCCTGACAATTCTTGATCC
GGTGCGCATTGAAGAAAATAGCCTGCGTAGCAGTGAGCAGGTACAGAAAAATGCGCTTCTTGGTAAAGTG
GCCGTGCTCGACCAGCACTATTACACCATCAGTGGTATCGACCCCCAGGCCGGGACAATGACCATCAAGG
ATAATGAGGGCAAGGTGCGACTCATCTCCAATATCGAAAACACCGCCCATGATCTCGCTATCTATCAACC
GAGGGAAATAAAGGTCAGTGAAGGCGATAAGATCCGCTTCACCCGCACCGTCAATGAACACGGCCATGTG
GCCAATAGCCAGTGGCAGGTAAAACGCATCGAGGATAATGGCGCGATAGTGCTGCACGATGGCAATAACG
AGAAAACCGTTCGCCCCGACCAATTTAAGGAAGACCAGCACATTGATTTGGGGTATGCCATTACCGCCTA
TGGTGCGCAAGGAGCGAGCAGTACCTACGCCATCGAATTACAGGCGATCAAAGGCGTAAATAGAAACTTG
ATTACCCTGTCGTCTGCGTATGTCCCCGCCTCACGAGCCAAAGAACATATGCAGACCTATACCGATGATA
AGGAAGGCTGGATTAACTTGCTCAATAAAAACCTCAAGAGCCAGGTTAGTACCGCCCACGATGCGCTCGA
AGGCGACCAGATTAAAGGGCTGAAGGTCGCCGATATGTTATTGGGTGCCGCCTCGCCGATGCGCTCCACA
GCATTGGGGCGACATGTGCTAAAGACCCATCAACTCGATGAGCAGCAGCATATGGGAAAATACATTGCAC
CAGGCAAAAAATACCCGACCCCGCACGCTGCCTTTCCGGTGTGGGATAATAATGGTAAACCGGCAGGGGC
TGCCATGGTGGAGCTGCAAAGCCATCACCAAAATAAAGGCCCTACCCTGGACAGTGAATATCGACTGGTG
GCAAATGAACGAGCCGAGTTTATCGGACTCCAGCGTGCCAGTAATGGCGAAACCCGTATCGCAACCAGTT
TAGAGGATGGCATTGCCATGGCGACCAAGCATCCACACAGTGGAATATTGGTCAGCATTAACGGCGCACC
ACCACCATTTAATGTCAATCGGATGACCGGGGGTAAATTGGTCGTGTCGGACACGGATGCGCAGCACCTG
CAAAAAGAGTCAGAGCGGGAGTTACCACTGCCACAAGATATGACCACCAAAGAAGCCAAACGCCAGGACG
AGATGATAAAAACCGTTGCCAGGGAGAAAGAACAGCCGGATATCAAACTGCCTGATGATAAACAGCAAGC
GGTCAATCGCGACGACAAGCGACTCCACAACGATATCCATCAACTAAATAAAGACGCGGAGTTAAGTGAG
CGACAATTGCGTAAGCTGGCAGAAATCAGAGACGTGGCCAAAGCCAAATTTACCCATGAACTCCACGACA
AACTGGATAAGCTGGAAAGGGAGATAATCAAGGAATTGACCAAAGGGGAGTAA
 Primer design