StrainAgrobacterium tumefaciens plasmid Ti plasmid pTiBo542 (NC_010929)
Repliconplasmid Ti plasmid pTiBo542 [Browse all T4SS(s) in this replicon]
Functioneffector translocation and conjugation
ClassificationType IVA; Type P
experimental Experimental investigation has been performed on this T4SS.

T4SS components

The information of T4SS components from NC_010929
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#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1pTiBo128148219..148605 [+], 387190014762orf_Bo128 
2pTiBo129148602..148946 [+], 345190014763orf_Bo129 
3pTiBo130149021..150610 [+], 1590190014764orf_Bo130 
4pTiBo131 (virJ)150873..151616 [+], 744190014765VirJ 
5pTiBo132151620..151997 [-], 378190014766orf_Bo132 
6pTiBo133 (virB1)153093..153812 [+], 720190014767type IV secretion system lytic transglycosylase VirB1  VirB1
7pTiBo134 (virB2)153827..154192 [+], 366190014768type IV secretion system pilin subunit VirB2  VirB2
8pTiBo135 (virB3)154192..154518 [+], 327190014769type IV secretion system protein VirB3  VirB3
9pTiBo136 (virB4)154518..156887 [+], 2370190014770type IV secretion system protein VirB4  VirB4
10pTiBo137 (virB5)156902..157564 [+], 663190014771type IV secretion system protein VirB5  VirB5
11pTiBo138 (virB6)157661..158548 [+], 888190014772type IV secretion system protein VirB6  VirB6
12pTiBo139 (virB7)158582..158749 [+], 168190014773type IV secretion system lipoprotein VirB7  VirB7
13pTiBo140 (virB8)158736..159449 [+], 714190014774type IV secretion system protein VirB8  VirB8
14pTiBo141 (virB9)159446..160327 [+], 882190014775type IV secretion system protein VirB9  VirB9
15pTiBo142 (virB10)160324..161457 [+], 1134190014776type IV secretion system protein VirB10  VirB10
16pTiBo143 (virB11)161497..162528 [+], 1032190014777type IV secretion system protein VirB11  VirB11
17pTiBo144 (virG)162660..163463 [+], 804190014778two-component response regulator VirG 
18pTiBo145 (virC2)163574..164182 [-], 609190014779putative crown gall tumor protein VirC2 
19pTiBo146 (virC1)164185..164880 [-], 696190014780putative crown gall tumor protein VirC1 
20pTiBo147 (virD1)165149..165592 [+], 444190014781type IV secretion system T-DNA border endonuclease VirD1 
21pTiBo148 (virD2)165626..166900 [+], 1275190014782type IV secretion system T-DNA border endonuclease VirD2 
22pTiBo149 (virD3)166920..167558 [+], 639190014783VirD3 
23pTiBo150 (virD4)167555..169513 [+], 1959190014784type IV secretion system protein VirD4  VirD4
24pTiBo151 (virD5)169614..172124 [+], 2511190014785VirD5 
25pTiBo152 (virE1)172574..172771 [+], 198190014786type IV secretion system chaperone VirE1 
26pTiBo153 (virE2)172776..174425 [+], 1650190014787type IV secretion system single-stranded DNA binding protein VirE2 
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

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Proteins        Genes
This system delivers both DNA and proteins into plant cell. The transferred DNA (T-DNA) region: NC_010929: 1..15730)

VirD2, VirD5, VirE2, VirE3, VirF

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1pTiBo148 (virD2)165626..166900 [+], 1275190014782type IV secretion system T-DNA border endonuclease VirD2  VirD2
2pTiBo151 (virD5)169614..172124 [+], 2511190014785VirD5  VirD5
3pTiBo153 (virE2)172776..174425 [+], 1650190014787type IV secretion system single-stranded DNA binding protein VirE2  VirE2
4pTiBo154 (virE3)174492..176510 [+], 2019190014788VirE3  VirE3
5pTiBo210 222375..223316 [+], 942190014842orf_Bo210  VirF

Download FASTA format files
Proteins        Genes
(1) Mossey P; Hudacek A; Das A (2010). Agrobacterium tumefaciens type IV secretion protein VirB3 is an inner membrane protein and requires VirB4, VirB7, and VirB8 for stabilization. J Bacteriol. 192(11):2830-8. [PudMed:20348257] experimental
experimental This literature contains experimental investigation