StrainAgrobacterium tumefaciens str. C58
Repliconplasmid Ti [Browse all T4SS(s) in this replicon]
Functioneffector translocation and conjugation
ClassificationType IVA; Type P
experimental Experimental investigation has been performed on this T4SS.

T4SS components

The information of T4SS components from NC_003065
Click the image to have a larger view
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1Atu6164 (tzs)179262..179993 [-], 73241223351trans-zeatin secretion protein 
2Atu6165180279..180470 [+], 192159161997hypothetical protein 
3Atu6166 (virA)180831..183332 [+], 250216119781two-component VirA-like sensor kinase 
4Atu6167 (virB1)183877..184614 [+], 73841223307type IV secretion system lytic transglycosylase VirB1  VirB1
5Atu6168 (virB2)184614..184979 [+], 36616119783type IV secretion system pilin subunit VirB2  VirB2
6Atu6169 (virB3)184979..185305 [+], 32716119784type IV secretion system protein VirB3  VirB3
7Atu6170 (virB4)185305..187674 [+], 237016119786type IV secretion system protein VirB4  VirB4
8Atu6171 (virB5)187691..188353 [+], 66316119787type IV secretion system protein VirB5  VirB5
9Atu6172 (virB6)188454..189341 [+], 88841223308type IV secretion system protein VirB6  VirB6
10Atu6173 (virB7)189377..189544 [+], 168159161998type IV secretion system lipoprotein VirB7  VirB7
11Atu6174 (virB8)189531..190244 [+], 71441223309type IV secretion system protein VirB8  VirB8
12Atu6175 (virB9)190241..191122 [+], 88216119791type IV secretion system protein VirB9  VirB9
13Atu6176 (virB10)191119..192252 [+], 113416119792type IV secretion system protein VirB10  VirB10
14Atu6177 (virB11)192293..193327 [+], 103541223310type IV secretion system protein VirB11  VirB11
15Atu6178 (virG)193546..194307 [+], 76216119794two-component response regulator VirG 
16Atu6179 (virC2)194449..195057 [-], 609159161999putative crown gall tumor protein VirC2 
17Atu6180 (virC1)195060..195755 [-], 69616119796putative crown gall tumor protein VirC1 
18Atu6181 (virD1)196026..196469 [+], 44441223311type IV secretion system T-DNA border endonuclease VirD1 
19Atu6182 (virD2)196503..197846 [+], 134416119798type IV secretion system T-DNA border endonuclease VirD2 
20Atu6183 (virD3)197861..199882 [+], 2022159162000virA/G regulated protein 
21Atu6184 (virD4)199956..201962 [+], 200741223312type IV secretion system protein VirD4  VirD4
22Atu6185 (virD5)202074..204596 [+], 252316119801virA/G regulated protein 
23Atu6186 (virE3)204666..205232 [+], 567159162001virA/G regulated protein 
24Atu6188 (virE0)205684..205935 [+], 252159162002virA/G regulated protein 
25Atu6189 (virE1)206051..206242 [+], 19216119804type IV secretion system chaperone VirE1 
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes
This system delivers both oncogenic DNA and proteins into plant cells. The transferred DNA (T-DNA, NC_003065 REGION: 1..24836) is processed by VirD1, VirD2, and VirC1 (Dtr proteins) and covalently bound to VirD2 to form a VirD2-T-strand transfer intermediate. The T-DNA complex is then transferred by this system into target cells. In addition, the T4SS independently translocates four additional effector proteins: VirE2, VirE3, VirF and VirD5.

VirD2, VirD5, VirE2, VirE3, VirF

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1Atu6182 (virD2)196503..197846 [+], 134416119798type IV secretion system T-DNA border endonuclease VirD2  VirD2
2Atu6185 (virD5)202074..204596 [+], 252316119801virA/G regulated protein  VirD5
3Atu6190 (virE2)206256..207926 [+], 167116119805type IV secretion system single-stranded DNA binding protein VirE2  VirE2
4Atu6191 (virE3)207993..210047 [+], 205516119806virA/G regulated protein  VirE3
5Atu6154 (virF)172489..173427 [+], 93916119971exported virulence protein  VirF

Download FASTA format files
Proteins        Genes
(1) Aguilar J; Cameron TA; Zupan J; Zambryski P (2011). Membrane and core periplasmic Agrobacterium tumefaciens virulence Type IV secretion system components localize to multiple sites around the bacterial perimeter during lateral attachment to plant cells. MBio. 2(6):e00218-11. [PudMed:22027007] experimental
(2) Sivanesan D; Baron C (2011). The dimer interface of Agrobacterium tumefaciens VirB8 is important for type IV secretion system function, stability, and association of VirB2 with the core complex. J Bacteriol. 193(9):2097-106. [PudMed:21398549] experimental
(3) Mossey P; Hudacek A; Das A (2010). Agrobacterium tumefaciens type IV secretion protein VirB3 is an inner membrane protein and requires VirB4, VirB7, and VirB8 for stabilization. J Bacteriol. 192(11):2830-8. [PudMed:20348257] experimental
(4) Aguilar J; Zupan J; Cameron TA; Zambryski PC (2010). Agrobacterium type IV secretion system and its substrates form helical arrays around the circumference of virulence-induced cells. Proc Natl Acad Sci U S A. 107(8):3758-63. [PudMed:20133577] experimental
(5) Zupan J; Hackworth CA; Aguilar J; Ward D; Zambryski P (2007). VirB1* promotes T-pilus formation in the vir-Type IV secretion system of Agrobacterium tumefaciens. J Bacteriol. 189(18):6551-63. [PudMed:17631630] experimental
(6) Draper O; Middleton R; Doucleff M; Zambryski PC (2006). Topology of the VirB4 C terminus in the Agrobacterium tumefaciens VirB/D4 type IV secretion system. J Biol Chem. 281(49):37628-35. [PudMed:17038312] experimental
(7) Wessel M; Klusener S; Godeke J; Fritz C; Hacker S; Narberhaus F (2006). Virulence of Agrobacterium tumefaciens requires phosphatidylcholine in the bacterial membrane. Mol Microbiol. 62(3):906-15. [PudMed:17010159] experimental
(8) McCullen CA; Binns AN (2006). Agrobacterium tumefaciens and plant cell interactions and activities required for interkingdom macromolecular transfer. Annu Rev Cell Dev Biol. 22:101-27. [PudMed:16709150]
(9) Bailey S; Ward D; Middleton R; Grossmann JG; Zambryski PC (2006). Agrobacterium tumefaciens VirB8 structure reveals potential protein-protein interaction sites. Proc Natl Acad Sci U S A. 103(8):2582-7. [PudMed:16481621] experimental
(10) Middleton R; Sjolander K; Krishnamurthy N; Foley J; Zambryski P (2005). Predicted hexameric structure of the Agrobacterium VirB4 C terminus suggests VirB4 acts as a docking site during type IV secretion. Proc Natl Acad Sci U S A. 102(5):1685-90. [PudMed:15668378] in_silico
(11) Ward DV; Draper O; Zupan JR; Zambryski PC (2002). Peptide linkage mapping of the Agrobacterium tumefaciens vir-encoded type IV secretion system reveals protein subassemblies. Proc Natl Acad Sci U S A. 99(17):11493-500. [PudMed:12177441] experimental
(12) Hapfelmeier S; Domke N; Zambryski PC; Baron C (2000). VirB6 is required for stabilization of VirB5 and VirB3 and formation of VirB7 homodimers in Agrobacterium tumefaciens. J Bacteriol. 182(16):4505-11. [PudMed:10913084] experimental
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation