SecReT4
T4SS ID4
StrainHelicobacter pylori 26695
Repliconchromosome [Browse all T4SS(s) in this replicon]
AccessionNC_000915
Location548134..579087
NameCag
Functioneffector translocation
ClassificationType IVA; Type P
experimental Experimental investigation has been performed on this T4SS.

T4SS components
ComponentCagCCagDCagECagFCagGCagHCagICagLCagN
Number111111111
ComponentCagMCagTCagUCagVCagWCagXCagYCagZCagalpha
Number111111111
ComponentCagbetaCaggammaCagdelta
Number111

The information of T4SS components from NC_000915
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#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1HP0517 (era)544336..545244 [+], 90915645144GTP-binding protein Era 
2HP0518545241..546233 [+], 99315645145hypothetical protein 
3HP0519546322..547152 [-], 83115645146hypothetical protein 
4HP0520547328..547675 [+], 34815645147cag pathogenicity island protein (cag1) 
5HP0522548134..549579 [+], 144615645148cag pathogenicity island protein (cag3)  Cagdelta
6HP0523549589..550098 [+], 51015645149cag pathogenicity island protein (cag4)  Caggamma
7HP0524550217..552463 [-], 224715645150cag pathogenicity island protein (cag5)  Cagbeta
8HP0525552472..553464 [-], 99315645151virB11-like protein  Cagalpha
9HP0526553469..554068 [-], 60015645152cag pathogenicity island protein (cag6)  CagZ
10HP0527554206..559989 [-], 578415645153cag pathogenicity island protein (cag7)  CagY
11HP0528560004..561572 [-], 156915645154cag pathogenicity island protein (cag8)  CagX
12HP0529561625..563232 [-], 160815645155cag pathogenicity island protein (cag9)  CagW
13HP0530563237..563995 [-], 75915645156cag pathogenicity island protein (cag10)  CagV
14HP0531564381..565037 [+], 65715645157cag pathogenicity island protein (cag11)  CagU
15HP0532565073..565915 [+], 84315645158cag pathogenicity island protein (cag12)  CagT
16HP0534566126..566716 [-], 59115645160cag pathogenicity island protein (cag13) 
17HP0535567142..567522 [-], 38115645161cag pathogenicity island protein (cag14) 
18HP0536567955..568299 [-], 34515645162cag pathogenicity island protein (cag15) 
19HP0537568723..569853 [+], 113115645163cag pathogenicity island protein (cag16)  CagM
20HP0538569868..570788 [+], 92115645164cag pathogenicity island protein (cag17)  CagN
21HP0539570870..571583 [-], 71415645165cag pathogenicity island protein (cag18)  CagL
22HP0540571580..572725 [-], 114615645166cag pathogenicity island protein (cag19)  CagI
23HP0541572736..573848 [-], 111315645167cag pathogenicity island protein (cag20)  CagH
24HP0542573864..574292 [-], 42915645168cag pathogenicity island protein (cag21)  CagG
25HP0543574347..575153 [-], 80715645169cag pathogenicity island protein (cag22)  CagF
26HP0544575155..578106 [-], 295215645170cag pathogenicity island protein (cag23)  CagE
27HP0545578115..578738 [-], 62415645171cag pathogenicity island protein (cag24)  CagD
28HP0546578740..579087 [-], 34815645172cag pathogenicity island protein (cag25)  CagC
29HP0547579921..583481 [+], 356115645173cag pathogenicity island protein (cag26) 
 
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

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Proteins        Genes
As a consequence of CagA action, epithelial cells will have some of their major functions disturbed, such as cell-cell adhesion, signalling, adherence and proliferation

Effectors
CagA; Peptidoglycan

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1HP0547 579921..583481 [+], 356115645173cag pathogenicity island protein (cag26)  CagA

Download FASTA format files
Proteins        Genes
(1) You Y; He L; Zhang M; Fu J; Gu Y; Zhang B; Tao X; Zhang J (2012). Comparative Genomics of Helicobacter pylori Strains of China Associated with Different Clinical Outcome. PLoS One. 7(6):e38528. [PudMed:22701658] experimental in_silico
(2) Jimenez-Soto LF; Rohrer S; Jain U; Ertl C; Sewald X; Haas R (2012). Effects of cholesterol on Helicobacter pylori growth and virulence properties in vitro. Helicobacter. 17(2):133-9. [PudMed:22404444] experimental
(3) Rizzato C; Torres J; Plummer M; Munoz N; Franceschi S; Camorlinga-Ponce M; Fuentes-Panana EM; Canzian F; Kato I (2012). Variations in Helicobacter pylori cytotoxin-associated genes and their influence in progression to gastric cancer: implications for prevention. PLoS One. 7(1):e29605. [PudMed:22235308] experimental in_silico
(4) Aviles-Jimenez F; Reyes-Leon A; Nieto-Patlan E; Hansen LM; Burgueno J; Ramos IP; Camorlinga-Ponce M; Bermudez H; Blancas JM; Cabrera L; Ribas-Aparicio RM; Solnick JV; Torres-Lopez J (2012). In vivo expression of Helicobacter pylori virulence genes in patients with gastritis, ulcer, and gastric cancer. Infect Immun. 80(2):594-601. [PudMed:22124657] experimental
(5) Lai CH; Wang HJ; Chang YC; Hsieh WC; Lin HJ; Tang CH; Sheu JJ; Lin CJ; Yang MS; Tseng SF; Wang WC (2011). Helicobacter pylori CagA-mediated IL-8 induction in gastric epithelial cells is cholesterol-dependent and requires the C-terminal tyrosine phosphorylation-containing domain. FEMS Microbiol Lett. 323(2):155-63. [PudMed:22092715] experimental
(6) Wang HJ; Cheng WC; Cheng HH; Lai CH; Wang WC (2012). Helicobacter pylori cholesteryl glucosides interfere with host membrane phase and affect type IV secretion system function during infection in AGS cells. Mol Microbiol. 83(1):67-84. [PudMed:22053852] experimental
(7) Backert S; Clyne M; Tegtmeyer N (2011). Molecular mechanisms of gastric epithelial cell adhesion and injection of CagA by Helicobacter pylori. Cell Commun Signal. 9:28. [PudMed:22044679]
(8) Mori N; Ishikawa C; Senba M (2011). Induction of CD69 expression by cagPAI-positive Helicobacter pylori infection. World J Gastroenterol. 17(32):3691-9. [PudMed:21990950] experimental
(9) Shaffer CL; Gaddy JA; Loh JT; Johnson EM; Hill S; Hennig EE; McClain MS; McDonald WH; Cover TL (2011). Helicobacter pylori exploits a unique repertoire of type IV secretion system components for pilus assembly at the bacteria-host cell interface. PLoS Pathog. 7(9):e1002237. [PudMed:21909278] experimental
(10) Backert S; Clyne M (2011). Pathogenesis of Helicobacter pylori infection. Helicobacter. 16 Suppl 1:19-25. [PudMed:21896081]
(11) Terradot L; Waksman G (2011). Architecture of the Helicobacter pylori Cag-type IV secretion system. FEBS J. 278(8):1213-22. [PudMed:21352491]
(12) Tegtmeyer N; Backert S (2011). Role of Abl and Src family kinases in actin-cytoskeletal rearrangements induced by the Helicobacter pylori CagA protein. Eur J Cell Biol. 90(11):880-90. [PudMed:21247656]
(13) Jurik A; Hausser E; Kutter S; Pattis I; Prassl S; Weiss E; Fischer W (2010). The coupling protein Cagbeta and its interaction partner CagZ are required for type IV secretion of the Helicobacter pylori CagA protein. Infect Immun. 78(12):5244-51. [PudMed:20876293] experimental
(14) Hutton ML; Kaparakis-Liaskos M; Turner L; Cardona A; Kwok T; Ferrero RL (2010). Helicobacter pylori exploits cholesterol-rich microdomains for induction of NF-kappaB-dependent responses and peptidoglycan delivery in epithelial cells. Infect Immun. 78(11):4523-31. [PudMed:20713621] experimental
(15) Tegtmeyer N; Hartig R; Delahay RM; Rohde M; Brandt S; Conradi J; Takahashi S; Smolka AJ; Sewald N; Backert S (2010). A small fibronectin-mimicking protein from bacteria induces cell spreading and focal adhesion formation. J Biol Chem. 285(30):23515-26. [PudMed:20507990] experimental
(16) Fischer W; Windhager L; Rohrer S; Zeiller M; Karnholz A; Hoffmann R; Zimmer R; Haas R (2010). Strain-specific genes of Helicobacter pylori: genome evolution driven by a novel type IV secretion system and genomic island transfer. Nucleic Acids Res. 38(18):6089-101. [PudMed:20478826] experimental in_silico
(17) Fehri LF; Rechner C; Janssen S; Mak TN; Holland C; Bartfeld S; Bruggemann H; Meyer TF (2009). Helicobacter pylori-induced modification of the histone H3 phosphorylation status in gastric epithelial cells reflects its impact on cell cycle regulation. Epigenetics. 4(8):577-86. [PudMed:20081355] experimental
(18) Brandt S; Kenny B; Rohde M; Martinez-Quiles N; Backert S (2009). Dual infection system identifies a crucial role for PKA-mediated serine phosphorylation of the EPEC-Tir-injected effector protein in regulating Rac1 function. Cell Microbiol. 11(8):1254-71. [PudMed:19438518] experimental
(19) Svensson H; Hansson M; Kilhamn J; Backert S; Quiding-Jarbrink M (2009). Selective upregulation of endothelial E-selectin in response to Helicobacter pylori-induced gastritis. Infect Immun. 77(7):3109-16. [PudMed:19414551] experimental
(20) Tegtmeyer N; Zabler D; Schmidt D; Hartig R; Brandt S; Backert S (2009). Importance of EGF receptor, HER2/Neu and Erk1/2 kinase signalling for host cell elongation and scattering induced by the Helicobacter pylori CagA protein: antagonistic effects of the vacuolating cytotoxin VacA. Cell Microbiol. 11(3):488-505. [PudMed:19046339] experimental
(21) Hatakeyama M (2008). Linking epithelial polarity and carcinogenesis by multitasking Helicobacter pylori virulence factor CagA. Oncogene. 27(55):7047-54. [PudMed:19029944]
(22) Kutter S; Buhrdorf R; Haas J; Schneider-Brachert W; Haas R; Fischer W (2008). Protein subassemblies of the Helicobacter pylori Cag type IV secretion system revealed by localization and interaction studies. J Bacteriol. 190(6):2161-71. [PudMed:18178731] experimental
(23) Minohara Y; Boyd DK; Hawkins HK; Ernst PB; Patel J; Crowe SE (2007). The effect of the cag pathogenicity island on binding of Helicobacter pylori to gastric epithelial cells and the subsequent induction of apoptosis. Helicobacter. 12(6):583-90. [PudMed:18001397] experimental
(24) Pattis I; Weiss E; Laugks R; Haas R; Fischer W (2007). The Helicobacter pylori CagF protein is a type IV secretion chaperone-like molecule that binds close to the C-terminal secretion signal of the CagA effector protein. Microbiology. 153(Pt 9):2896-909. [PudMed:17768234] experimental
(25) Poppe M; Feller SM; Romer G; Wessler S (2007). Phosphorylation of Helicobacter pylori CagA by c-Abl leads to cell motility. Oncogene. 26(24):3462-72. [PudMed:17160020] experimental
(26) Oyarzabal OA; Rad R; Backert S (2007). Conjugative transfer of chromosomally encoded antibiotic resistance from Helicobacter pylori to Campylobacter jejuni. J Clin Microbiol. 45(2):402-8. [PudMed:17135441] experimental
(27) Oliveira MJ; Costa AC; Costa AM; Henriques L; Suriano G; Atherton JC; Machado JC; Carneiro F; Seruca R; Mareel M; Leroy A; Figueiredo C (2006). Helicobacter pylori induces gastric epithelial cell invasion in a c-Met and type IV secretion system-dependent manner. J Biol Chem. 281(46):34888-96. [PudMed:16990273] experimental
(28) Andrzejewska J; Lee SK; Olbermann P; Lotzing N; Katzowitsch E; Linz B; Achtman M; Kado CI; Suerbaum S; Josenhans C (2006). Characterization of the pilin ortholog of the Helicobacter pylori type IV cag pathogenicity apparatus, a surface-associated protein expressed during infection. J Bacteriol. 188(16):5865-77. [PudMed:16885455] experimental
(29) Busler VJ; Torres VJ; McClain MS; Tirado O; Friedman DB; Cover TL (2006). Protein-protein interactions among Helicobacter pylori cag proteins. J Bacteriol. 188(13):4787-800. [PudMed:16788188] experimental
(30) Hohlfeld S; Pattis I; Puls J; Plano GV; Haas R; Fischer W (2006). A C-terminal translocation signal is necessary, but not sufficient for type IV secretion of the Helicobacter pylori CagA protein. Mol Microbiol. 59(5):1624-37. [PudMed:16469000] experimental
(31) Krueger S; Hundertmark T; Kalinski T; Peitz U; Wex T; Malfertheiner P; Naumann M; Roessner A (2006). Helicobacter pylori encoding the pathogenicity island activates matrix metalloproteinase 1 in gastric epithelial cells via JNK and ERK. J Biol Chem. 281(5):2868-75. [PudMed:16321971] experimental
(32) Backert S; Kwok T; Konig W (2005). Conjugative plasmid DNA transfer in Helicobacter pylori mediated by chromosomally encoded relaxase and TraG-like proteins. Microbiology. 151(Pt 11):3493-503. [PudMed:16272373] experimental
(33) Zahrl D; Wagner M; Bischof K; Bayer M; Zavecz B; Beranek A; Ruckenstuhl C; Zarfel GE; Koraimann G (2005). Peptidoglycan degradation by specialized lytic transglycosylases associated with type III and type IV secretion systems. Microbiology. 151(Pt 11):3455-67. [PudMed:16272370] experimental
(34) Backert S; Gressmann H; Kwok T; Zimny-Arndt U; Konig W; Jungblut PR; Meyer TF (2005). Gene expression and protein profiling of AGS gastric epithelial cells upon infection with Helicobacter pylori. Proteomics. 5(15):3902-18. [PudMed:16145711] experimental
(35) Bauer B; Moese S; Bartfeld S; Meyer TF; Selbach M (2005). Analysis of cell type-specific responses mediated by the type IV secretion system of Helicobacter pylori. Infect Immun. 73(8):4643-52. [PudMed:16040977] experimental
(36) Viala J; Chaput C; Boneca IG; Cardona A; Girardin SE; Moran AP; Athman R; Memet S; Huerre MR; Coyle AJ; DiStefano PS; Sansonetti PJ; Labigne A; Bertin J; Philpott DJ; Ferrero RL (2004). Nod1 responds to peptidoglycan delivered by the Helicobacter pylori cag pathogenicity island. Nat Immunol. 5(11):1166-74. [PudMed:15489856] experimental
(37) Selbach M; Moese S; Backert S; Jungblut PR; Meyer TF (2004). The Helicobacter pylori CagA protein induces tyrosine dephosphorylation of ezrin. Proteomics. 4(10):2961-8. [PudMed:15378755] experimental
(38) Al-Ghoul L; Wessler S; Hundertmark T; Kruger S; Fischer W; Wunder C; Haas R; Roessner A; Naumann M (2004). Analysis of the type IV secretion system-dependent cell motility of Helicobacter pylori-infected epithelial cells. Biochem Biophys Res Commun. 322(3):860-6. [PudMed:15336542] experimental
(39) Aras RA; Fischer W; Perez-Perez GI; Crosatti M; Ando T; Haas R; Blaser MJ (2003). Plasticity of repetitive DNA sequences within a bacterial (Type IV) secretion system component. J Exp Med. 198(9):1349-60. [PudMed:14581606] experimental
(40) Buhrdorf R; Forster C; Haas R; Fischer W (2003). Topological analysis of a putative virB8 homologue essential for the cag type IV secretion system in Helicobacter pylori. Int J Med Microbiol. 293(2-3):213-7. [PudMed:12868658] experimental
(41) Rohde M; Puls J; Buhrdorf R; Fischer W; Haas R (2003). A novel sheathed surface organelle of the Helicobacter pylori cag type IV secretion system. Mol Microbiol. 49(1):219-34. [PudMed:12823823] experimental
(42) Tanaka J; Suzuki T; Mimuro H; Sasakawa C (2003). Structural definition on the surface of Helicobacter pylori type IV secretion apparatus. Cell Microbiol. 5(6):395-404. [PudMed:12780777] experimental
(43) Savvides SN; Yeo HJ; Beck MR; Blaesing F; Lurz R; Lanka E; Buhrdorf R; Fischer W; Haas R; Waksman G (2003). VirB11 ATPases are dynamic hexameric assemblies: new insights into bacterial type IV secretion. EMBO J. 22(9):1969-80. [PudMed:12727865] experimental
(44) Backert S; Churin Y; Meyer TF (2002). Helicobacter pylori type IV secretion, host cell signalling and vaccine development. Keio J Med. 51 Suppl 2:6-14. [PudMed:12528929] experimental
(45) Schroder G; Krause S; Zechner EL; Traxler B; Yeo HJ; Lurz R; Waksman G; Lanka E (2002). TraG-like proteins of DNA transfer systems and of the Helicobacter pylori type IV secretion system: inner membrane gate for exported substrates. J Bacteriol. 184(10):2767-79. [PudMed:11976307] experimental
(46) Fischer W; Puls J; Buhrdorf R; Gebert B; Odenbreit S; Haas R (2001). Systematic mutagenesis of the Helicobacter pylori cag pathogenicity island: essential genes for CagA translocation in host cells and induction of interleukin-8. Mol Microbiol. 42(5):1337-48. [PudMed:11886563] experimental
(47) Selbach M; Moese S; Meyer TF; Backert S (2002). Functional analysis of the Helicobacter pylori cag pathogenicity island reveals both VirD4-CagA-dependent and VirD4-CagA-independent mechanisms. Infect Immun. 70(2):665-71. [PudMed:11796597] experimental
(48) Churin Y; Kardalinou E; Meyer TF; Naumann M (2001). Pathogenicity island-dependent activation of Rho GTPases Rac1 and Cdc42 in Helicobacter pylori infection. Mol Microbiol. 40(4):815-23. [PudMed:11401689] experimental
(49) Backert S; Ziska E; Brinkmann V; Zimny-Arndt U; Fauconnier A; Jungblut PR; Naumann M; Meyer TF (2000). Translocation of the Helicobacter pylori CagA protein in gastric epithelial cells by a type IV secretion apparatus. Cell Microbiol. 2(2):155-64. [PudMed:11207572] experimental
(50) Ramarao N; Gray-Owen SD; Backert S; Meyer TF (2000). Helicobacter pylori inhibits phagocytosis by professional phagocytes involving type IV secretion components. Mol Microbiol. 37(6):1389-404. [PudMed:10998171] experimental
 
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation