SecReT4
T4SS ID526
StrainNovosphingobium aromaticivorans plasmid pNL1 (NC_002033)
Repliconplasmid pNL1 [Browse all T4SS(s) in this replicon]
AccessionNC_002033
Location90847..125874
NameTra_F
Functionconjugation
ClassificationType IVA; Type F
experimental Experimental investigation has been performed on this T4SS.

T4SS components
ComponentTraA_FTraB_FTraC_FTraD_FTraE_FTraF_FTraG_FTraH_FTraI_F
Number011111111
ComponentTraK_FTraL_FTraM_FTraN_FTraQ_FTraU_FTraV_FTraW_FTraX_F
Number110101110
ComponentTraY_FTrbC_FOrf169_F
Number011

The information of T4SS components from NC_002033
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#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1pNL1_p084 (orf659)87091..87834 [-], 74410956912hypothetical protein 
2pNL1_p085 (orf666)87971..89014 [+], 104410956913hypothetical protein 
3pNL1_p086 (orf679)89068..89451 [+], 38410956914hypothetical protein 
4pNL1_p087 (orf680)89448..89759 [+], 31210956915hypothetical protein 
5pNL1_p088 (orf681)89819..90265 [+], 44710956916hypothetical protein 
6pNL1_p089 (orf690)90570..90860 [+], 29110956917hypothetical protein 
7pNL1_p090 (traD)90847..93177 [+], 233110956918Conjugal DNA metabolism  TraD_F
8pNL1_p091 (orf704)93181..96222 [+], 304210956919DNA helicase  TraI_F
9pNL1_p092 (orf721)96464..96667 [+], 20410956920hypothetical protein 
10pNL1_p093 (orf724)96687..97043 [+], 35710956921hypothetical protein 
11pNL1_p094 (orf729)97152..99593 [+], 244210956922hypothetical protein 
12pNL1_p095 (orf753)99699..99914 [+], 21610956923hypothetical protein 
13pNL1_p096 (orf756)100124..100345 [+], 22210956924hypothetical protein 
14pNL1_p097 (orf758)100348..101148 [-], 80110956925regulator 
15pNL1_p098 (orf766)101252..101968 [+], 71710956926hypothetical protein 
16pNL1_p099 (orf771)102073..102294 [+], 22210956927hypothetical protein 
17pNL1_p100 (isA)102735..103681 [+], 94710956930transposase, fusion orf777 and orf780 
18pNL1_p101 (isAb)102735..103121 [+], 38710956928transposase N-terminus 
19pNL1_p102 (isAa)103208..103681 [+], 47410956929transposase C-terminus 
20pNL1_p103 (traG)103653..106391 [-], 273910956931sex pilus assembly and mating pair stabilization protein  TraG_F
21pNL1_p104 (traH)106404..107858 [-], 145510956932pilus assembly and synthesis protein  TraH_F
22pNL1_p105 (traF)107858..108670 [-], 81310956933pilus assembly and synthesis protein  TraF_F
23pNL1_p106 (orf816)108667..109077 [-], 41110956934hypothetical protein 
24pNL1_p107 (traN)109058..111172 [-], 211510956935mating pair stabilization protein  TraN_F
25pNL1_p108 (trbC)111174..111938 [-], 76510956936sex pilus assembly and mating pair formation protein  TrbC_F
26pNL1_p109 (traU)111947..112969 [-], 102310956937sex pilus assembly and synthesis protein  TraU_F
27pNL1_p110 (orf843)112966..113232 [-], 26710956938hypothetical protein 
28pNL1_p111 (traW)113229..113924 [-], 69610956939sex pilus assembly and synthesis protein  TraW_F
29pNL1_p112 (orf851)113921..115027 [-], 110710956940Conjugal transfer protein 
30pNL1_p113 (orf857)115017..115379 [-], 36310956941hypothetical protein 
31pNL1_p114 (traC)115376..117946 [-], 257110956942sex pilus assembly and synthesis protein  TraC_F
32pNL1_p115 (orf876)118097..119140 [-], 104410956943hypothetical protein  TraV_F
33pNL1_p116 (orf883)119137..120033 [-], 89710956944protein disulfide-isomerase 
34pNL1_p117 (traB)120044..121420 [-], 137710956945sex pilus assembly and synthesis protein  TraB_F
35pNL1_p118 (traK)121420..122214 [-], 79510956946sex pilus assembly and synthesis protein  TraK_F
36pNL1_p119 (traE)122214..122837 [-], 62410956947sex pilus assembly and synthesis protein  TraE_F
37pNL1_p120 (traL)122851..123135 [-], 28510956948sex pilus assembly and synthesis protein  TraL_F
38pNL1_p121 (orf912)123201..123515 [-], 31510956949hypothetical protein 
39pNL1_p122 (orf916)123680..124213 [+], 53410956951hypothetical protein 
40pNL1_p123 (orf915)124303..124824 [+], 52210956950hypothetical protein 
41pNL1_p124 (orf921)124835..125110 [+], 27610956952hypothetical protein 
42pNL1_p125 (orf925)125077..125874 [-], 79810956953transglycosylase  Orf169_F
43pNL1_p126 (orf933)126055..126723 [-], 66910956954hypothetical protein 
44pNL1_p127 (orf938)126716..127414 [-], 69910956955hypothetical protein 
45pNL1_p128 (orf942)127407..127799 [-], 39310956956hypothetical protein 
46pNL1_p129 (orf946)127877..128887 [-], 101110956957hypothetical protein 
47pNL1_p130 (bphA2e)128947..129444 [-], 49810956958small subunit aromatic oxygenase 
48pNL1_p131 (bphA1e)129441..130616 [-], 117610956959large subunit aromatic oxygenase 
 
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

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Proteins        Genes
(1) Romine MF; Stillwell LC; Wong KK; Thurston SJ; Sisk EC; Sensen C; Gaasterland T; Fredrickson JK; Saffer JD (1999). Complete sequence of a 184-kilobase catabolic plasmid from Sphingomonas aromaticivorans F199. J Bacteriol. 181(5):1585-602. [PudMed:10049392]