SecReT4
T4SS ID6
StrainAgrobacterium tumefaciens plasmid pTi-SAKURA (NC_002147)
Repliconplasmid pTi-SAKURA [Browse all T4SS(s) in this replicon]
AccessionNC_002147
Location140576..158661
NameVirB
Functioneffector translocation and conjugation
ClassificationType IVA; Type P
sequence This T4SS has been mentioned in a genome sequencing report.

T4SS components
ComponentVirB1VirB2VirB3VirB4VirB5VirB6VirB7VirB8VirB9
Number111111111
ComponentVirB10VirB11VirD4
Number111

The information of T4SS components from NC_002147
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#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1pTi-SAKURA_p141 (tiorf139)135962..136693 [-], 73210954959hypothetical protein 
2pTi-SAKURA_p142 (tiorf140)137530..140031 [+], 250210954960two-component VirA-like sensor kinase 
3pTi-SAKURA_p143 (tiorf141)140576..141313 [+], 73810954961type IV secretion system lytic transglycosylase VirB1  VirB1
4pTi-SAKURA_p144 (tiorf142)141313..141678 [+], 36610954962type IV secretion system pilin subunit VirB2  VirB2
5pTi-SAKURA_p145 (tiorf143)141678..142004 [+], 32710954963type IV secretion system protein VirB3  VirB3
6pTi-SAKURA_p146 (tiorf144)142004..144373 [+], 237010954964type IV secretion system protein VirB4  VirB4
7pTi-SAKURA_p147 (tiorf145)144390..145052 [+], 66310954965type IV secretion system protein VirB5  VirB5
8pTi-SAKURA_p148 (tiorf146)145153..146040 [+], 88810954966type IV secretion system protein VirB6  VirB6
9pTi-SAKURA_p149 (tiorf147)146076..146243 [+], 16810954967type IV secretion system lipoprotein VirB7  VirB7
10pTi-SAKURA_p150 (tiorf148)146230..146943 [+], 71410954968type IV secretion system protein VirB8  VirB8
11pTi-SAKURA_p151 (tiorf149)146940..147821 [+], 88210954969type IV secretion system protein VirB9  VirB9
12pTi-SAKURA_p152 (tiorf150)147818..148951 [+], 113410954970type IV secretion system protein VirB10  VirB10
13pTi-SAKURA_p153 (tiorf151)148992..150026 [+], 103510954971type IV secretion system protein VirB11  VirB11
14pTi-SAKURA_p154 (tiorf152)150245..151006 [+], 76210954972two-component response regulator VirG 
15pTi-SAKURA_p155 (tiorf153)151148..151756 [-], 60910954973putative crown gall tumor protein VirC2 
16pTi-SAKURA_p156 (tiorf154)151759..152454 [-], 69610954974putative crown gall tumor protein VirC1 
17pTi-SAKURA_p157 (tiorf155)152725..153168 [+], 44410954975type IV secretion system T-DNA border endonuclease VirD1 
18pTi-SAKURA_p158 (tiorf156)153202..154545 [+], 134410954976type IV secretion system T-DNA border endonuclease VirD2 
19pTi-SAKURA_p159 (tiorf157)154560..156581 [+], 202210954977hypothetical protein 
20pTi-SAKURA_p160 (tiorf158)156655..158661 [+], 200710954978type IV secretion system protein VirD4  VirD4
21pTi-SAKURA_p161 (tiorf159)158773..161298 [+], 252610954979hypothetical protein 
22pTi-SAKURA_p162 (tiorf160)161675..162001 [-], 32710954980hypothetical protein 
23pTi-SAKURA_p163 (tiorf161)162387..162638 [+], 25210954981hypothetical protein 
24pTi-SAKURA_p164 (tiorf162)162754..162945 [+], 19210954982type IV secretion system chaperone VirE1 
 
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes
This system delivers both DNA and proteins into plant cell. The transferred DNA (T-DNA) region: NC_002147: 170935..197050)

Effectors
VirF, VirD2, VirD5, VirE2, VirE3

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1pTi-SAKURA_p135 (tiorf133)129189..130127 [+], 93910954953hypothetical protein  VirF
2pTi-SAKURA_p158 (tiorf156)153202..154545 [+], 134410954976type IV secretion system T-DNA border endonuclease VirD2  VirD2
3pTi-SAKURA_p161 (tiorf159)158773..161298 [+], 252610954979hypothetical protein  VirD5
4pTi-SAKURA_p165 (tiorf163)162959..164629 [+], 167110954983type IV secretion system single-stranded DNA binding protein VirE2  VirE2
5pTi-SAKURA_p166 (tiorf164)164744..166750 [+], 200710954984hypothetical protein  VirE3

Download FASTA format files
Proteins        Genes
(1) Hattori Y; Iwata K; Suzuki K; Uraji M; Ohta N; Katoh A; Yoshida K (2001). Sequence characterization of the vir region of a nopaline type Ti plasmid, pTi-SAKURA. Genes Genet Syst. 76(2):121-30. [PudMed:11434457]
(2) Suzuki K; Hattori Y; Uraji M; Ohta N; Iwata K; Murata K; Kato A; Yoshida K (2000). Complete nucleotide sequence of a plant tumor-inducing Ti plasmid. Gene. 242(1-2):331-6. [PudMed:10721727]
(3) Suzuki K; Hattori Y; Uraji M; Ohta N; Katoh A; Yoshida K (1997). Genome structure of pTi-SAKURA (II): genetic map constructed by complete DNA sequencing. Nucleic Acids Symp Ser. (37):161-2. [PudMed:9586049]