I. Summary
SecReT6 ID151 experimental
StrainPseudomonas aeruginosa UCBPP-PA14
Repliconchromosome [Browse all T6SS(s) in this replicon]
CommentNeeded for full virulence in mouse lung and acute burn model; required for wild-type infection of Arabidopsis
Functionanti-eukaryotic; virulence
T6SS TypeType i; subtype i4a
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
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III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1PA14_338303007457..3007780 [+], 324116050325hypothetical protein 
2PA14_338403007822..3008742 [+], 921116050324transcriptional regulator 
3PA14_33860 (lldA)3008839..3009990 [+], 1152116050323L-lactate dehydrogenase 
4PA14_338703010057..3010299 [-], 243116050322hypothetical protein 
5PA14_338803010594..3010830 [+], 237116050321hypothetical protein 
6PA14_338903011096..3011566 [+], 471116050320oxidoreductase 
7PA14_339003011563..3013878 [+], 2316116050319aldehyde dehydrogenase 
8PA14_339103014295..3015569 [-], 1275116050318hypothetical protein 
9PA14_339203015701..3016342 [-], 642116050317transcriptional regulator 
10PA14_339303016619..3017014 [+], 396116050316hypothetical protein 
11PA14_339403017037..3017573 [-], 537116050315hypothetical protein 
12PA14_339603017584..3019590 [-], 2007116050314hypothetical protein  TssI
13PA14_339703019627..3020694 [-], 1068116050313hypothetical protein 
14PA14_339803020733..3021266 [-], 534116050312hypothetical protein 
15PA14_339903021340..3023889 [-], 2550116050311ClpA/B-type protease  TssH
16PA14_340003023891..3024907 [-], 1017116050310hypothetical protein  TssG
17PA14_340103024871..3026664 [-], 1794116050309hypothetical protein  TssF
18PA14_340203026648..3027073 [-], 426116050308hypothetical protein  TssE
19PA14_340303027086..3027583 [-], 498116050307hypothetical protein  TssD
20PA14_340503027657..3029141 [-], 1485116050306hypothetical protein  TssC
21PA14_340703029164..3029709 [-], 546116050305hypothetical protein  TssB
22PA14_340803029917..3030393 [+], 477116050304hypothetical protein 
23PA14_341003030453..3031784 [+], 1332116050303hypothetical protein  TssK
24PA14_341103031802..3032560 [+], 759116050302hypothetical protein  TssL
25PA14_341303032557..3036372 [+], 3816116050301hypothetical protein  TssM
26PA14_341403036369..3037469 [+], 1101116050300hypothetical protein  TssA
27PA14_341503037579..3038664 [+], 1086116050299transcriptional regulator  Sfa3
28PA14_341703038777..3039166 [+], 390116050298hypothetical protein 
29PA14_34180 (msuE)3039326..3039886 [+], 561116050297NADH-dependent FMN reductase MsuE 
30PA14_34190 (msuD)3039896..3041041 [+], 1146116050296methanesulfonate sulfonatase MsuD 
31PA14_342003041071..3042255 [+], 1185116050295FMNH2-dependent monooxygenase 
32PA14_342103042252..3043382 [+], 1131116050294transcriptional regulator 
33PA14_342303043554..3044708 [+], 1155116050293hypothetical protein 
34PA14_342503044774..3045901 [-], 1128116050292glycerophosphoryl diester phosphodiesterase 
35PA14_342603046079..3046732 [-], 654116050291ABC transporter permease 
36PA14_342703046716..3047825 [-], 1110116050290ABC transporter ATP-binding protein 
37PA14_342803047822..3048616 [-], 795116050289hypothetical protein 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA14_672205999498..6001735 [-], 2238116053236hypothetical protein PA5089

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Proteins        Genes

Homology Immunity protein list
PA5087, PA5088, PA5087, PA5088

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA14_671805995985..5996854 [-], 870116053232hypothetical protein PA5087
2PA14_671905996863..5997744 [-], 882116053233hypothetical protein PA5088
3PA14_672005997741..5998610 [-], 870116053234hypothetical protein PA5087
4PA14_672105998619..5999494 [-], 876116053235hypothetical protein PA5088

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Proteins        Genes
Regulatory element identified
LasR (PA14_45960)ActivatorPMID: 19497948

MvfR (PA14_51340)ActivatorPMID: 19497948

PqsE (PA14_51380)ActivatorPMID: 19497948

(1) Barret M et al. (2011). Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537] in_silico
(2) Lesic B et al. (2009). Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology. 155(Pt 9):2845-55. [PudMed:19497948] experimental
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation