SecReT6
I. Summary
SecReT6 ID26 experimental
StrainRalstonia solanacearum GMI1000
Repliconplasmid pGMI1000MP [Browse all T6SS(s) in this replicon]
AccessionNC_003296
Location926494..971950
CommentTssB of R. solanacearum was required for secretion of Hcp, the hemolysin coregulated protein and a hallmark of T6S pathway. Deletion of tssB in R. solanacearum GMI1000 strain resulted in defect of biofilm formation, and the expression of the flagella operon is decreased, leading to decreased motility. More importantly, tssB mutant strain had significantly attenuated its virulence on tomato plants.
Functionanti-eukaryotic; effector translocation
T6SS TypeType i; subtype i4b
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number111311111
ComponentTssGTssJTssKTssM
Number1111
Click the above image to have a larger view
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1RS05792 (RSp0733)922097..923044 [-], 94817548954hypothetical protein 
2RS05791 (RSp0734)923063..923980 [-], 91817548955hypothetical protein 
3RS01974 (RSp0735)924024..925265 [-], 124217548956hypothetical protein 
4RS01973 (RSp0736)925361..925795 [-], 43517548957transmembrane protein 
5RS01972 (RSp0737)925792..926493 [-], 70217548958hypothetical protein 
6RS01970 (RSp0738)926494..929190 [-], 269717548959VGR-related protein  TssI
7RS01969 (RSp0739)929249..930040 [-], 79217548960hypothetical protein  TssL
8RS01968 (RSp0740)930037..931383 [-], 134717548961hypothetical protein  TssK
9RS01967 (RSp0741)931427..932008 [-], 58217548962transmembrane protein  TssJ
10RS01966 (RSp0742)932376..933023 [+], 64817548963lipoprotein 
11RS01965 (RSp0743)933060..933572 [+], 51317548964hypothetical protein  TssB
12RS01964 (RSp0744)933565..935055 [+], 149117548965hypothetical protein  TssC
13RS01963 (RSp0745)935144..935647 [+], 50417548966hypothetical protein  TssD
14RS01962 (RSp0746)935708..936181 [+], 47417548967hypothetical protein  TssE
15RS01961 (RSp0747)936244..938094 [+], 185117548968hypothetical protein  TssF
16RS01960 (RSp0748)938058..939149 [+], 109217548969hypothetical protein  TssG
17RS01959 (RSp0749)939182..941899 [+], 271817548970CLPA/B-type chaperone protein  TssH
18RS01958 (RSp0750)941920..944676 [+], 275717548971VGR-related protein  TssI
19RS01957 (RSp0751)944694..945827 [+], 113417548972hypothetical protein 
20RS01956 (RSp0752)945805..948582 [+], 277817548973transmembrane protein 
21RS01955 (RSp0753)948595..949701 [+], 110717548974hypothetical protein 
22RS01954 (RSp0754)949909..950547 [+], 63917548975hypothetical protein 
23RS01953 (RSp0755)950752..953901 [+], 315017548976hypothetical protein 
24RS01952 (RSp0756)954467..954850 [+], 38417548977hypothetical protein 
25RS01951 (RSp0757)954855..955613 [+], 75917548978hypothetical protein 
26RS01950 (RSp0758)955610..956953 [+], 134417548979hypothetical protein 
27RS01949 (RSp0759)957153..958166 [+], 101417548980hypothetical protein  TssA
28RS01948 (RSp0760)958239..959237 [-], 99917548981hypothetical protein 
29RS01947 (RSp0761)959306..960139 [-], 83417548982lipoprotein  TagL
30RS01946 (RSp0762)960142..961128 [-], 98717548983hypothetical protein 
31RS01945 (RSp0763)961125..965066 [-], 394217548984hypothetical protein  TssM
32RS01944 (RSp0764)965104..966591 [-], 148817548985hypothetical protein 
33RS01943 (RSp0765)966602..966871 [-], 27017548986hypothetical protein  PAAR
34RS01942 (RSp0766)966911..967759 [-], 84917548987hypothetical protein 
35RS01941 (RSp0767)968046..968792 [+], 74717548988hypothetical protein 
36RS01940 (RSp0768)968930..971950 [+], 302117548989VGR-related protein  TssI
37RS01939 (RSp0769)971966..973459 [+], 149417548990transmembrane protein 
38RS01938 (RSp0770)973634..974650 [+], 101717548991hypothetical protein 
39RS01937 (RSp0771)974849..975778 [+], 93017548992hypothetical protein 
40RS01936 (RSp0772)975792..976862 [+], 107117548993signal peptide protein 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
BCAL0343

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1RS01963(RSp0745)935144..935647 [+], 50417548966hypothetical protein BCAL0343

Download FASTA format files
Proteins        Genes
N/A
(1) Zhang L et al. (2014). TssB is essential for virulence and required for Type VI secretion system in Ralstonia solanacearum. Microb Pathog. 74C:1-7. [PudMed:24972114] experimental
 
experimental This literature contains experimental investigation