SecReT6
I. Summary
SecReT6 ID3 experimental
StrainPseudomonas aeruginosa PAO1
Repliconchromosome (H1-T6SS) [Browse all T6SS(s) in this replicon]
AccessionNC_002516
Location83380..123495
CommentVirulence in rat model of chronic respiratory infection; Ability to outcompete a susceptible mutant of P. aeruginosa; ability to outcompete P. putida; growth inhibition of E. coli
Functionanti-bacterial and anti-eukaryotic; effector translocation;virulence
T6SS TypeType i; subtype i3
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number111211111
ComponentTssGTssJTssKTssM
Number1111
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III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1PA0067 (prlC)78710..80755 [+], 204615595265oligopeptidase A 
2PA006880752..81027 [+], 27615595266hypothetical protein 
3PA006981116..82174 [+], 105915595267hypothetical protein 
4PA007082404..83318 [-], 91515595268hypothetical protein  TagQ
5PA007183380..85092 [-], 171315595269hypothetical protein  TagR
6PA007285085..86284 [-], 120015595270hypothetical protein  TagS
7PA007386284..87003 [-], 72015595271ABC transporter ATP-binding protein  TagT
8PA0074 (ppkA)87000..90098 [-], 309915595272serine/threonine protein kinase PpkA  PpkA
9PA0075 (pppA)90106..90834 [-], 72915595273PppA  PppA
10PA007690844..91524 [-], 68115595274hypothetical protein  TagF
11PA0077 (icmF1)91521..94826 [-], 330615595275IcmF1  TssM
12PA007895048..96397 [-], 135015595276hypothetical protein  TssL
13PA007996404..97738 [-], 133515595277hypothetical protein  TssK
14PA008097754..98218 [-], 46515595278hypothetical protein  TssJ
15PA0081 (fha1)98263..99756 [-], 149415595279Fha1  Fha
16PA0082100124..101158 [+], 103515595280hypothetical protein  TssA
17PA0083101247..101765 [+], 51915595281hypothetical protein  TssB
18PA0084101778..103274 [+], 149715595282hypothetical protein  TssC
19PA0085 (hcp1)103350..103838 [+], 48915595283hypothetical protein  TssD
20PA0086104006..104851 [+], 84615595284hypothetical protein  TagJ
21PA0087104853..105362 [+], 51015595285hypothetical protein  TssE
22PA0088105359..107218 [+], 186015595286hypothetical protein  TssF
23PA0089107182..108228 [+], 104715595287hypothetical protein  TssG
24PA0090 (clpV1)108221..110929 [+], 270915595288ClpV1 protein  TssH
25PA0091 (vgrG1)110976..112907 [+], 193215595289VgrG1  TssI
26PA0092113022..113306 [-], 28515595290hypothetical protein  Tsi6 (i)
27PA0093113303..114595 [-], 129315595291hypothetical protein  Tse6 (e)
28PA0094114611..115045 [-], 43515595292hypothetical protein 
29PA0095115299..117524 [+], 222615595293hypothetical protein  TssI
30PA0096117552..118001 [+], 45015595294hypothetical protein 
31PA0097117931..119130 [+], 120015595295hypothetical protein 
32PA0098119127..120164 [+], 1038155952963-oxoacyl-ACP synthase 
33PA0099120164..121324 [+], 116115595297hypothetical protein  PA0099 (e)
34PA0100121346..122266 [+], 92115595298hypothetical protein 
35PA0101122248..123495 [+], 124815595299hypothetical protein 
36PA0102123871..124599 [+], 72915595300carbonic anhydrase 
37PA0103124810..126381 [+], 157215595301sulfate transporter 
38PA0104126518..127114 [+], 59715595302hypothetical protein 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
PA0085 , PA0091, PA0093 (Tse6), PA0095, PA0099, PA1844 (Tse1, Tae1), PA2684 (Tse5), PA2685, PA2702 (Tse2), PA2774 (Tse4), PA3484 (Tse3, Tge1)

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA0085(hcp1)103350..103838 [+], 48915595283hypothetical protein PA0085
2PA0091(vgrG1)110976..112907 [+], 193215595289VgrG1 PA0091
3PA0093(Tse6)113303..114595 [-], 129315595291hypothetical protein (Tse6)PA0093 (Tse6)
4PA0095115299..117524 [+], 222615595293hypothetical protein PA0095
5PA0099120164..121324 [+], 116115595297hypothetical protein PA0099
6PA1844(Tse1)2003868..2004332 [-], 46515597041hypothetical protein (Tse1, Tae1)PA1844 (Tse1, Tae1)
7PA2684(Tse5)3031749..3035702 [-], 395415597880hypothetical protein (Tse5)PA2684 (Tse5)
8PA26853035752..3037932 [-], 218115597881hypothetical protein PA2685
9PA2702(Tse2)3056890..3057366 [+], 47715597898hypothetical protein (Tse2)PA2702 (Tse2)
10PA2774(Tse4)3132229..3132816 [-], 58815597970hypothetical protein (Tse4)PA2774 (Tse4)
11PA3484(Tse3)3898278..3899504 [+], 122715598680hypothetical protein (Tse3, Tge1)PA3484 (Tse3, Tge1)
Data from experimental literature

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Proteins        Genes

Homology Immunity protein list
PA0092 (Tsi6), PA1845 (Tsi1,Tai1), PA2703 (Tsi2), PA2775 (Tsi4), PA3485 (Tsi3,Tgi1)   PA2684.1 (Tsi5)

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA0092113022..113306 [-], 28515595290hypothetical protein (Tsi6)PA0092 (Tsi6)
2PA1845(Tsi1)2004374..2004892 [-], 51915597042hypothetical protein (Tsi1,Tai1)PA1845 (Tsi1,Tai1)
3PA2703(Tsi2)3057376..3057609 [+], 23415597899hypothetical protein (Tsi2)PA2703 (Tsi2)
4PA27753132821..3133258 [-], 43815597971hypothetical protein (Tsi4)PA2775 (Tsi4)
5PA3485(Tsi3)3899501..3899938 [+], 43815598681hypothetical protein (Tsi3,Tgi1)PA3485 (Tsi3,Tgi1)
Data from experimental literature

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Proteins        Genes
Regulatory element identified
PpkA
RegulatorDescriptionReference
PpkA (PA0074)Activator: FhaPMID: 17558395

Gac/Rsm
RegulatorDescriptionReference
Gac/RsmIndirect activatorPMID: 21955777

LasR
RegulatorDescriptionReference
LasR (PA1430)Indirect repressorPMID: 19497948

MvfR
RegulatorDescriptionReference
MvfR (PA1003)Indirect repressorPMID: 15686549

TagR
RegulatorDescriptionReference
TagR (PA0071)Activator: ppkAPMID: 19400797

RetS
RegulatorDescriptionReference
RetS (PA4856)RepressorPMID: 16763151

LadS
RegulatorDescriptionReference
LadS (PA3974)ActivatorPMID: 16763151

PppA
RegulatorDescriptionReference
PppA (PA0075)Repressor: FhaPMID: 17558395

TagF
RegulatorDescriptionReference
TagF (PA0076)RepressorPMID: 22017253

WspR
RegulatorDescriptionReference
WspR (PA3702)ActivatorPMID: 21955777

Subinhibitory Level Kanamycin
RegulatorDescriptionReference
Subinhibitory Level KanamycinIndirect activatorPMID: 24260549

PA2133
RegulatorDescriptionReference
PA2133RepressorPMID: 21955777

tpbB
RegulatorDescriptionReference
tpbB (PA1120)ActivatorPMID: 21955777

AmpR
RegulatorDescriptionReference
AmpR (PA4109)RepressorPMID: 24291602

RsmY/RsmZ sRNAs
RegulatorDescriptionReference
RsmY/RsmZ sRNAsActivatorPMID: 24169572

SuhB
RegulatorDescriptionReference
SuhB (PA3818)Indirect repressorPMID: 24169572

AmrZ
RegulatorDescriptionReference
AmrZ (PA3385)Activator: PA0082 (TssA)PMID: 24603766

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experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation