SecReT6
I. Summary
SecReT6 ID372 experimental
StrainEdwardsiella tarda EIB202
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNC_013508
Location2555437..2575336
CommentVirulence in blue gourami fish; virulence in several other fish models; invasion of cultured carp epithelial cells
Functionanti-eukaryotic;effector translocation;adherence;virulence
T6SS TypeType i; subtype i4b
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number111111111
ComponentTssGTssJTssKTssM
Number1111
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III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1ETAE_24232550717..2550998 [-], 282269139768hypothetical protein 
2ETAE_24242551037..2551585 [-], 549269139769transporting ATPase 
3ETAE_24252551686..2552486 [-], 801269139770hypothetical protein 
4ETAE_24262552790..2554019 [-], 1230269139771cell wall-associated hydrolase 
5ETAE_24272554393..2554524 [-], 132269139772hypothetical protein 
6ETAE_2428 (evpP)2554834..2555136 [+], 303269139773type VI secretion system protein EvpP  EvpP (e)
7ETAE_24292555437..2555901 [+], 465269139774hypothetical protein  TssB
8ETAE_2430 (evpB)2555898..2557385 [+], 1488269139775type VI secretion system protein EvpB  TssC
9ETAE_2431 (evpC)2557455..2557946 [+], 492269139776type VI secretion system protein EvpC  TssD
10ETAE_2432 (evpD)2558046..2559248 [+], 1203269139777type VI secretion system protein EvpD 
11ETAE_2433 (evpE)2559389..2559733 [+], 345269139778type VI secretion system protein EvpE  TssE
12ETAE_2434 (evpF)2559737..2561578 [+], 1842269139779type VI secretion system protein EvpF  TssF
13ETAE_2435 (evpG)2561575..2562600 [+], 1026269139780type VI secretion system protein EvpG  TssG
14ETAE_2436 (evpH)2562719..2565331 [+], 2613269139781type VI secretion system protein EvpH  TssH
15ETAE_2437 (evpI)2565397..2567316 [+], 1920269139782type VI secretion system protein EvpI  TssI
16ETAE_2438 (evpJ)2567411..2567713 [+], 303269139783type VI secretion system protein EvpJ  PAAR
17ETAE_2439 (evpK)2567727..2568794 [+], 1068269139784type VI secretion system protein EvpK  TssA
18ETAE_2440 (evpL)2568781..2569422 [+], 642269139785type VI secretion system protein EvpL  TssJ
19ETAE_2441 (evpM)2569522..2570910 [+], 1389269139786type VI secretion system protein EvpM  TssK
20ETAE_2442 (evpN)2570907..2571557 [+], 651269139787type VI secretion system protein EvpN  TssL
21ETAE_2443 (evpO)2571554..2575336 [+], 3783269139788type VI secretion system protein EvpO  TssM
22ETAE_2444 (mepA)2575468..2576298 [-], 831269139789penicillin-insensitive murein endopeptidase 
23ETAE_2445 (aroC)2576312..2577397 [-], 1086269139790chorismate synthase 
24ETAE_24462577489..2578421 [-], 933269139791N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase 
25ETAE_24472578596..2579177 [+], 582269139792hypothetical protein 
26ETAE_2448 (sixA)2579228..2579704 [-], 477269139793phosphohistidine phosphatase 
27ETAE_24492580096..2580320 [-], 225269139794hypothetical protein 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
EvpP , EvpC, EvpI

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1ETAE_2428(evpP)2554834..2555136 [+], 303269139773type VI secretion system protein EvpP EvpP
2ETAE_2431(evpC)2557455..2557946 [+], 492269139776type VI secretion system protein EvpC EvpC
3ETAE_2437(evpI)2565397..2567316 [+], 1920269139782type VI secretion system protein EvpI EvpI
Data from experimental literature

Download FASTA format files
Proteins        Genes
N/A
Regulatory element identified
Fur
RegulatorDescriptionReference
Fur (ETAE_2610)ActivatorPMID: 21953460

PhoB-PhoR
RegulatorDescriptionReference
PhoB (ETAE_1080)ActivatorPMID: 21953460
PhoR (ETAE_1081)Indirect activatorPMID: 21953460

EsrA
RegulatorDescriptionReference
EsrA (ETAE_0885)Indirect activatorPMID: 20832475

EsrB
RegulatorDescriptionReference
EsrB (ETAE_0886)Indirect activatorPMID: 20832475

EsrC
RegulatorDescriptionReference
EsrC (ETAE_0861)ActivatorPMID: 20832475

PhoP-PhoQ
RegulatorDescriptionReference
PhoP (ETAE_2059)ActivatorPMID: 22227416
PhoQ (ETAE_2060)Indirect activatorPMID: 22227416

H-NS
RegulatorDescriptionReference
H-NS (ETAE_1505)RepressorPMID: 25131176
H-NS (ETAE_2968)RepressorPMID: 25131176

(1) Zhang J et al. (2014). A new target for the old regulator: H-NS suppress T6SS secretory protein EvpP, the major virulence factor in the fish pathogen Edwardsiella tarda. Lett Appl Microbiol. 59(5):557-564. [PudMed:25131176] experimental
(2) Yang M et al. (2012). Edwardsiella comparative phylogenomics reveal the new intra/inter-species taxonomic relationships, virulence evolution and niche adaptation mechanisms. PLoS One. 7(5):e36987. [PudMed:22590641] in_silico
(3) Lv Y et al. (2012). Systematic mutation analysis of two-component signal transduction systems reveals EsrA-EsrB and PhoP-PhoQ as the major virulence regulators in Edwardsiella tarda. Vet Microbiol. 157(1-2):190-9. [PudMed:22227416] experimental
(4) Chakraborty S et al. (2011). Two-component PhoB-PhoR regulatory system and ferric uptake regulator sense phosphate and iron to control virulence genes in type III and VI secretion systems of Edwardsiella tarda. J Biol Chem. 286(45):39417-30. [PudMed:21953460]
(5) Leung KY et al. (2012). Edwardsiella tarda - virulence mechanisms of an emerging gastroenteritis pathogen. Microbes Infect. 14(1):26-34. [PudMed:21924375]
(6) Jobichen C et al. (2010). Structural basis for the secretion of EvpC: a key type VI secretion system protein from Edwardsiella tarda. PLoS One. 5(9):e12910. [PudMed:20886112]
(7) Wang X et al. (2010). Hemolysin EthA in Edwardsiella tarda is essential for fish invasion in vivo and in vitro and regulated by two-component system EsrA-EsrB and nucleoid protein HhaEt. Fish Shellfish Immunol. 29(6):1082-91. [PudMed:20832475] experimental
(8) Wang X et al. (2009). Edwardsiella tarda T6SS component evpP is regulated by esrB and iron, and plays essential roles in the invasion of fish. Fish Shellfish Immunol. 27(3):469-77. [PudMed:19563898]
(9) Zheng J et al. (2007). Dissection of a type VI secretion system in Edwardsiella tarda. Mol Microbiol. 66(5):1192-206. [PudMed:17986187] experimental
 
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation