SecReT6
I. Summary
SecReT6 ID551 experimental
StrainSalmonella enterica subsp. enterica serovar Typhimurium str. 14028S
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNC_016856
Location305365..332747
CommentRequired for full virulence in mice; gastrointestinal colonization and systemic spread of Salmonella enterica serovar Typhimurium in the chicken
Functionanti-eukaryotic; virulence
T6SS TypeType i; subtype i3
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number121111111
ComponentTssGTssJTssKTssM
Number1111
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III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1STM14_0305 (dniR)300623..301990 [-], 1368378448569membrane-bound lytic murein transglycosylase D 
2STM14_0306 (gloB)302062..302817 [-], 756378448570hydroxyacylglutathione hydrolase 
3STM14_0307 (yafS)302852..303574 [+], 723378448571putative SAM-dependent methyltransferase 
4STM14_0308 (rnhA)303571..304038 [-], 468378448572ribonuclease H 
5STM14_0309 (dnaQ)304102..304833 [+], 732378448573DNA polymerase III subunit epsilon 
6STM14_0310304859..304966 [-], 108378448574hypothetical protein 
7STM14_0312305167..305274 [+], 108378448575hypothetical protein 
8STM14_0313305365..306420 [-], 1056378448576putative cytoplasmic protein  TssA
9STM14_0314306431..307426 [-], 996378448577putative cytoplasmic protein  TssG
10STM14_0315307423..309306 [-], 1884378448578putative cytoplasmic protein  TssF
11STM14_0316309322..309816 [-], 495378448579putative cytoplasmic protein  TssE
12STM14_0317309813..310637 [-], 825378448580putative cytoplasmic protein  TagJ
13STM14_0318310624..311526 [-], 903378448581putative cytoplasmic protein 
14STM14_0319311894..314533 [+], 2640378448582putative chaperone ATPase  TssH
15STM14_0320314633..315175 [+], 543378448583putative cytoplasmic protein  TssB
16STM14_0321315199..316692 [+], 1494378448584putative cytoplasmic protein  TssC
17STM14_0322316755..316865 [+], 111378448585invasol SirA 
18STM14_0323316878..317324 [+], 447378448586putative cytoplasmic protein 
19STM14_0324317578..318063 [+], 486378448587putative cytoplasmic protein  TssD
20STM14_0325318368..318853 [+], 486378448588putative cytoplasmic protein  STM0277 (e)
21STM14_0326318838..319221 [+], 384378448589hypothetical protein  STM0278 (i)
22STM14_0327319364..319849 [+], 486378448590putative cytoplasmic protein  TssD
23STM14_0328319916..320452 [+], 537378448591putative outer membrane lipoprotein  TssJ
24STM14_0329320456..321799 [+], 1344378448592putative cytoplasmic protein  TssK
25STM14_0330321796..323100 [+], 1305378448593hypothetical protein  TssL
26STM14_0331323105..323878 [+], 774378448594putative inner membrane protein 
27STM14_0332324081..324512 [+], 432378448595putative Shiga-like toxin A subunit 
28STM14_0333324546..328415 [+], 3870378448596putative inner membrane protein  TssM
29STM14_0334328415..329203 [+], 789378448597putative cytoplasmic protein 
30STM14_0335329200..329616 [+], 417378448598hypothetical protein 
31STM14_0336329640..330161 [+], 522378448599putative cytoplasmic protein 
32STM14_0337330303..330512 [+], 210378448600hypothetical protein 
33STM14_0338330558..332747 [+], 2190378448601putative cytoplasmic protein  TssI
34STM14_0339332771..333217 [+], 447378448602putative cytoplasmic protein 
35STM14_0340333236..337330 [+], 4095378448603putative RHS-like protein  PAAR
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
SciK , STM0277, SciK, STY2606, Hcp

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1STM14_0324317578..318063 [+], 486378448587putative cytoplasmic protein SciK
2STM14_0325318368..318853 [+], 486378448588putative cytoplasmic protein STM0277
3STM14_0327319364..319849 [+], 486378448590putative cytoplasmic protein SciK
4STM14_29242538467..2538835 [+], 369378451100putative cytoplasmic protein STY2606
5STM14_37853309225..3309710 [-], 486378451925putative cytoplasmic protein Hcp

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Proteins        Genes

Homology Immunity protein list
STM0278, STY2607

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1STM14_0326318838..319221 [+], 384378448589hypothetical protein STM0278
2STM14_29252538835..2539353 [+], 519378451101hypothetical protein STY2607

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Proteins        Genes
(1) Pezoa D et al. (2013). The Type VI Secretion System Encoded in SPI-6 Plays a Role in Gastrointestinal Colonization and Systemic Spread of Salmonella enterica serovar Typhimurium in the Chicken. PLoS One. 8(5):e63917. [PudMed:23691117] experimental
(2) Liu J et al. (2012). The type VI secretion system gene cluster of Salmonella typhimurium: required for full virulence in mice. J Basic Microbiol. 53(7):600-7. [PudMed:22961625] experimental
 
experimental This literature contains experimental investigation