SecReT6
I. Summary
SecReT6 ID611 insolico
StrainPseudomonas aeruginosa M18
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNC_017548
Location89946..115973
Comment
FunctionN/A
T6SS TypeType i; subtype i3
insolico Bioinformatics investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number111211111
ComponentTssGTssJTssKTssM
Number1111
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III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1PAM18_0077 (pppA)88531..89259 [-], 729386056146PppA  PppA
2PAM18_007889269..89949 [-], 681386056147hypothetical protein  TagF
3PAM18_0079 (icmF1)89946..93476 [-], 3531386056148IcmF1  TssM
4PAM18_008093473..94822 [-], 1350386056149hypothetical protein  TssL
5PAM18_008194829..96163 [-], 1335386056150hypothetical protein  TssK
6PAM18_008296179..96643 [-], 465386056151hypothetical protein  TssJ
7PAM18_0083 (fha1)96688..98205 [-], 1518386056152Fha1  Fha
8PAM18_008498573..99607 [+], 1035386056153hypothetical protein  TssA
9PAM18_008599696..100214 [+], 519386056154hypothetical protein  TssB
10PAM18_0086100227..101723 [+], 1497386056155hypothetical protein  TssC
11PAM18_0087 (hcp1)101811..102287 [+], 477386056156hypothetical protein  TssD
12PAM18_0088102515..103300 [+], 786386056157hypothetical protein  TagJ
13PAM18_0089103302..103811 [+], 510386056158hypothetical protein  TssE
14PAM18_0090103808..105667 [+], 1860386056159hypothetical protein  TssF
15PAM18_0091105631..106677 [+], 1047386056160hypothetical protein  TssG
16PAM18_0092 (clpV1)106670..109378 [+], 2709386056161ClpV1 protein  TssH
17PAM18_0093 (vgrG1)109416..111356 [+], 1941386056162VgrG1  TssI
18PAM18_0094111471..111755 [-], 285386056163hypothetical protein  Tsi6 (i)
19PAM18_0095111752..113044 [-], 1293386056164hypothetical protein  Tse6 (e)
20PAM18_0096113060..113494 [-], 435386056165hypothetical protein 
21PAM18_0097113748..115973 [+], 2226386056166hypothetical protein  TssI
22PAM18_0098116001..116450 [+], 450386056167hypothetical protein 
23PAM18_0099116467..117579 [+], 1113386056168hypothetical protein 
24PAM18_0100117576..118613 [+], 10383860561693-oxoacyl-(acyl carrier protein) synthase 
25PAM18_0101118613..119773 [+], 1161386056170hypothetical protein  PA0099 (e)
26PAM18_0102119795..120715 [+], 921386056171hypothetical protein 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
PA0085 , PA0091, PA0093, PA0095, PA0099, PA3484, PA2774, PA2702, PA2685, PA2684, PA1844

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PAM18_0087(hcp1)101811..102287 [+], 477386056156hypothetical protein PA0085
2PAM18_0093(vgrG1)109416..111356 [+], 1941386056162VgrG1 PA0091
3PAM18_0095111752..113044 [-], 1293386056164hypothetical protein PA0093
4PAM18_0097113748..115973 [+], 2226386056166hypothetical protein PA0095
5PAM18_0101118613..119773 [+], 1161386056170hypothetical protein PA0099
6PAM18_14811629184..1630410 [-], 1227386057548hypothetical protein PA3484
7PAM18_21892386674..2387261 [+], 588386058252hypothetical protein PA2774
8PAM18_22702461803..2462279 [-], 477386058333hypothetical protein PA2702
9PAM18_22872481311..2483380 [+], 2070386058350hypothetical protein PA2685
10PAM18_22882483430..2487383 [+], 3954386058351hypothetical protein PA2684
11PAM18_31983575434..3575898 [+], 465386059261hypothetical protein PA1844

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Proteins        Genes

Homology Immunity protein list
PA0092, PA3485, PA2775, PA2703, Tsi5, PA1845

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PAM18_0094111471..111755 [-], 285386056163hypothetical protein PA0092
2PAM18_14801628750..1629187 [-], 438386057547hypothetical protein PA3485
3PAM18_21882386232..2386669 [+], 438386058251hypothetical protein PA2775
4PAM18_22692461560..2461793 [-], 234386058332hypothetical protein PA2703
5PAM18_22892487380..2487571 [+], 192386058352hypothetical protein Tsi5
6PAM18_31973574874..3575392 [+], 519386059260hypothetical protein PA1845

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Proteins        Genes
Regulatory element identified
GacA
RegulatorDescriptionReference
GacA (PAM18_2407)ActivatorPMID: 23708134

(1) Wei X et al. (2013). Global control of GacA on secondary metabolism, primary metabolism, secretion systems, and motility in the rhizobacterium Pseudomonas aeruginosa M18. J Bacteriol. 195(15):3387-400. [PudMed:23708134] experimental
 
experimental This literature contains experimental investigation