I. Summary

SecReT6 ID	715
Strain	Pseudomonas putida HB3267
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_019905
Location	3090329..3115594
Comment
Function	N/A
T6SS Type	Type i; subtype i4b
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	2

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III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	B479_14055	3089228..3089689 [-], 462	431802745	hypothetical protein
2	B479_14060	3089730..3090179 [-], 450	431802746	hypothetical protein
3	B479_14065	3090329..3092182 [-], 1854	431802747	Rhs element Vgr protein	TssI
4	B479_14070	3092797..3093099 [+], 303	431802748	hypothetical protein
5	B479_14075	3093297..3093845 [+], 549	431802749	hypothetical protein
6	B479_14080	3093873..3094442 [+], 570	431802750	hypothetical protein
7	B479_14085	3094866..3095630 [+], 765	431802751	hypothetical protein
8	B479_14090	3095659..3096234 [+], 576	431802752	type VI secretion protein	TssB
9	B479_14095	3096249..3097751 [+], 1503	431802753	EvpB family type VI secretion protein	TssC
10	B479_14100	3097778..3098260 [+], 483	431802754	type VI secretion system lysozyme-like protein	TssE
11	B479_14105	3098260..3100080 [+], 1821	431802755	type VI secretion protein	TssF
12	B479_14110	3100044..3101114 [+], 1071	431802756	hypothetical protein	TssG
13	B479_14115	3101111..3103708 [+], 2598	431802757	type VI secretion ATPase	TssH
14	B479_14120	3103733..3104455 [+], 723	431802758	putative lipoprotein	TssJ
15	B479_14125	3104452..3105795 [+], 1344	431802759	type VI secretion protein	TssK
16	B479_14130	3105792..3106508 [+], 717	431802760	hypothetical protein	TssL
17	B479_14135	3106539..3110342 [+], 3804	431802761	ImcF domain-containing protein	TssM
18	B479_14140	3110339..3111247 [+], 909	431802762	hypothetical protein
19	B479_14145	3111244..3113739 [+], 2496	431802763	OmpA/MotB domain-containing protein	TssM
20	B479_14150	3113883..3114401 [+], 519	431802764	Hcp1 family type VI secretion system effector	TssD
21	B479_14155	3114497..3115594 [+], 1098	431802765	ImpA family type VI secretion-associated protein	TssA
22	B479_14160	3115750..3118266 [+], 2517	431802766	excinuclease ABC subunit A
23	B479_14165	3118426..3120075 [+], 1650	431802767	Major facilitator transporter

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

Psyr_4965

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	B479_14150	3113883..3114401 [+], 519	431802764	Hcp1 family type VI secretion system effector	Psyr_4965

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Proteins        Genes

N/A

N/A