SecReT6
SecReT6 ID788 experimental
StrainAeromonas hydrophila SSU
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionDQ667172
Location1..27577
CommentCytotoxicity and inhibition of phagocytosis in cultured macrophages and epithelial cells; full virulence in septicaemic mouse model
Functionanti-eukaryotic; effector translocation;virulence
T6SS TypeType i; subtype i1
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number111220111
ComponentTssGTssJTssKTssM
Number1111
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III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1110277123 (hcp2)1..519 [+], 519110277123Hcp-2 hemolysin-coregulated protein  TssD
2110277124 (vgrG2)1004..3049 [+], 2046110277124VgrG-2 protein  TssI
31102771253049..3918 [+], 870110277125hypothetical protein 
41102771263915..4559 [+], 645110277126hypothetical protein 
51102771274660..5631 [+], 972110277127hypothetical protein  TssC
61102771285638..6069 [+], 432110277128hypothetical protein  TssE
7110277129 (vasA)6073..7839 [+], 1767110277129VasA  TssF
81102771307803..8801 [+], 999110277130hypothetical protein  TssG
91102771318858..10138 [+], 1281110277131hypothetical protein  Fha
1011027713210138..10653 [+], 516110277132hypothetical protein  TssJ
1111027713310656..11990 [+], 1335110277133hypothetical protein  TssK
12110277134 (vasF)12082..12792 [+], 711110277134VasF  TssL
13110277135 (clpB)12813..15455 [+], 2643110277135ClpB protein  TssH
14110277136 (vasH)15458..16996 [+], 1539110277136VasH 
1511027713716996..17601 [+], 606110277137hypothetical protein 
1611027713817610..19055 [+], 1446110277138hypothetical protein  TssA
17110277139 (vasK)19097..22582 [+], 3486110277139VasK  TssM
1811027714022629..24059 [+], 1431110277140hypothetical protein  TssA
1911027714124317..24604 [+], 288110277141hypothetical protein  PAAR
20110277142 (vgrG3)24615..26663 [+], 2049110277142VgrG-3 protein  TssI
2111027714326704..27577 [+], 874110277143hypothetical protein 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
   VgrG2 (ABG57133)   VgrG3 (ABG57151)   Hcp2 (ABG57132)   Hcp1 (AFX59894)   VgrG1 (AFX59895)
Homology effector (effector protein label, when available)
Homology immunity protein (immunity protein label, when available)
Regulatory element identified
AhyR
RegulatorDescriptionReference
AhyIActivatorPMID: 19729404
AhyRActivatorPMID: 19729404

VasH
RegulatorDescriptionReference
VasHActivatorPMID: 18037263

(1) Grim CJ et al. (2014). Functional Genomic Characterization of Virulence Factors from Necrotizing Fasciitis-Causing Strains of Aeromonas hydrophila. Appl Environ Microbiol. 80(14):4162-83. [PudMed:24795370] experimental
(2) Sha J et al. (2013). Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology. 159(Pt 6):1120-35. [PudMed:23519162] experimental
(3) Suarez G et al. (2010). Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology. 156(Pt 12):3678-88. [PudMed:20798163] experimental
(4) Suarez G et al. (2010). A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 192(1):155-68. [PudMed:19880608] experimental
(5) Suarez G et al. (2008). Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 44(4):344-61. [PudMed:18037263] experimental
 
experimental This literature contains experimental investigation