I. Information of ICE
ICEberg ID440
Name ICEPmu1 This ICE is derived from experimental literature
ICEO ID ICEO_0000181
OrganismPasteurella multocida 36950
Size (bp)82214
GC content [Genome] (%)41.9
Insertion sitetRNA-Leu [DRs: GATTTTGAATCAA]
FunctionHarbored 11 resistance genes, which confer resistance to streptomycin/spectinomycin (aadA25), streptomycin (strA and strB), gentamicin (aadB), kanamycin/neomycin (aphA1), tetracycline [tetR-tet (H)], chloramphenicol/florfenicol (floR), sulphonamides (sul2), tilmicosin/clindamycin [erm (42)] or tilmicosin/tulathromycin [msr (E)- mph (E)]
Species that ICE can be transferred toEscherichia coli HK22511; Pasteurella multocida E348-08 (capsular type F); Mannheimia haemolytica 39229
Nucleotide SequenceCP003022 (complete ICE sequence in this genome)
Genome coordinates273284..355497 
Putative oriT region -
Putative relaxase coordinates: 293488..295467; Locus tag: Pmu_02890;  Family:  MOBH

II. ICE interaction with IME/CIME/

The interaction information of ICEPmu1 is not available.

The graph information of ICEPmu1 components from CP003022
Complete gene list of ICEPmu1 from CP003022
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
1Pmu_02660272295..272966 [-], 672putative lipoprotein
2Pmu_02680273706..274284 [+], 579hypothetical protein
3Pmu_02690275016..275453 [-], 438putative phage transposase
4Pmu_02700275603..276502 [+], 900tyrosine recombinase-1 family proteinIntegrase 
5Pmu_02710276775..277698 [-], 924ISApl1 transposase
6aphA1279777..280592 [-], 816aminoglycoside 3'-phosphotransferase protein
7strB280928..281764 [-], 837aminoglycoside 6-phosphotransferase protein
8strA281764..282522 [-], 759aminoglycoside 3''-phosphotransferase protein
9sul2282735..283439 [-], 705dihydropteroate synthase
10Pmu_02790283743..285035 [+], 1293ISCR21 transposase
11floR286824..288038 [+], 1215florfenicol / chloramphenicol efflux protein
12Pmu_02830288066..288371 [+], 306putative LysR transcriptional regulator, HTH domain profile
13Pmu_02840288483..289976 [+], 1494ISCR2 transposase
14erm(42)290396..291301 [+], 906rRNA (adenine-N6-)-methyltransferase
15Pmu_02880292568..293332 [-], 765tyrosine recombinase-2 family protein
16Pmu_02890293488..295467 [-], 1980integrating conjugative element relaxase, PFGI-1 classRelaxase, MOBH Family
17Pmu_02900295895..296692 [-], 798putative type I restriction-modification system methyltransferase subunit
18Pmu_02910296765..297511 [-], 747hypothetical protein
19Pmu_02920297651..298625 [-], 975hypothetical protein
20Pmu_02930298870..299277 [-], 408hypothetical protein
21Pmu_02940299393..300235 [-], 843hypothetical protein
22Pmu_02950300313..300708 [-], 396hypothetical protein
23Pmu_02960300807..301052 [-], 246hypothetical protein
24Pmu_02970301124..301477 [-], 354hypothetical protein
25Pmu_02980301851..302294 [+], 444integrating conjugative element protein, PFL familyTfc24, T4SS component 
26Pmu_02990302291..303232 [+], 942integrating conjugative element protein, PFL familyTfc23, T4SS component 
27Pmu_03000303250..305262 [+], 2013integrating conjugative element protein, PFL familyTfc22, T4SS component 
28Pmu_03010305283..305711 [+], 429hypothetical proteinTfc21, T4SS component 
29Pmu_03020305927..306397 [+], 471hypothetical protein
30Pmu_03030306418..306759 [+], 342hypothetical proteinTfc20, T4SS component 
31Pmu_03040306805..308277 [-], 1473TraG-like domain-containing proteinTfc19, T4SS component 
32Pmu_03050308288..308605 [-], 318hypothetical proteinTfc18, T4SS component 
33Pmu_03060308595..309023 [-], 429hypothetical proteinTfc17, T4SS component 
34Pmu_03070309023..311860 [-], 2838conjugative transfer ATPase TraC-like, PFL familyTfc16, T4SS component 
35Pmu_03080311863..312261 [-], 399conjugative transfer region lipoproteinTfc15, T4SS component 
36Pmu_03090312274..313728 [-], 1455integrating conjugative element protein, PFL familyTfc14, T4SS component 
37Pmu_03100313740..314624 [-], 885integrating conjugative element protein, PFL familyTfc13, T4SS component 
38Pmu_03110314624..315268 [-], 645integrating conjugative element protein, PFL familyTfc12, T4SS component 
39Pmu_03120315272..315637 [-], 366conjugative transfer region proteinTfc11, T4SS component 
40Pmu_03130315660..316049 [-], 390integrating conjugative element protein, PFL familyTfc10, T4SS component 
41Pmu_03140316064..316297 [-], 234hypothetical protein
42Pmu_03150316297..316623 [-], 327integrative conjugative element protein, RAQPRD familyTfc9, T4SS component 
43Pmu_03160316810..317193 [+], 384hypothetical protein
44Pmu_03170317225..317920 [-], 696integrating conjugative element membrane protein, PFL familyTfc8, T4SS component 
45Pmu_03180317917..318270 [-], 354hypothetical proteinTfc7, T4SS component 
46Pmu_03190318263..320464 [-], 2202conjugative coupling factor TraD, SXT/TOL subfamilyTfc6, T4SS component 
47Pmu_03200320464..320970 [-], 507integrating conjugative element protein, PFL familyTfc5, T4SS component 
48Pmu_03210320973..321743 [-], 771putative lysozyme-like superfamilyTfc4, T4SS component 
49Pmu_03220321722..322495 [-], 774integrating conjugative element protein, PFL familyTfc3, T4SS component 
50Pmu_03230322495..323124 [-], 630putative integrating conjugative element protein PilL, PFGI-1 classTfc2, T4SS component 
51Pmu_03240323283..323543 [-], 261hypothetical protein
52Pmu_03250323656..324018 [-], 363hypothetical protein
53Pmu_03260324098..324505 [-], 408hypothetical protein
54Pmu_03270324531..325211 [-], 681hypothetical protein
55Pmu_03280325317..325748 [-], 432hypothetical protein
56topB1326566..328617 [-], 2052DNA topoisomerase III
57Pmu_03300328777..329295 [-], 519hypothetical protein
58Pmu_03310329392..329676 [-], 285hypothetical protein
59Pmu_03320329772..330482 [+], 711hypothetical protein
60Pmu_03330331021..331788 [-], 768aldo/keto reductase
61Pmu_03340331787..332107 [+], 321hypothetical protein
62Pmu_03350332289..332744 [+], 456hypothetical protein
63Pmu_03360332758..334305 [+], 1548multicopper oxidase protein
64Pmu_03370334395..334550 [-], 156alcohol dehydrogenase, zinc-binding domain protein
65Pmu_03380334639..334818 [+], 180hypothetical protein
66Pmu_03390334867..335385 [+], 519hypothetical protein
67tetR1335388..336011 [-], 624tetracycline repressor protein
68aadB336102..336731 [+], 630aminoglycoside 2''-O-adenyltransferase protein
69aadA25336789..337580 [+], 792aminoglycoside 3''-O-adenyltransferase protein
70blaOXA-2337657..338484 [+], 828beta-lactamase OXA-2 protein
71Pmu_03450338575..339279 [+], 705IS26 transposase
72msr(E)339642..341117 [+], 1476macrolide efflux protein
73mph(E)341173..342057 [+], 885macrolide 2'-phosphotransferase protein
74Pmu_03480342141..342845 [+], 705IS26 transposase
75tetR2343099..343722 [-], 624tetracycline repressor protein
76tet(H)343814..345016 [+], 1203tetracycline efflux protein, class H
77Pmu_03510345112..346422 [-], 1311transposase tnpA
78Pmu_03520346422..346724 [-], 303hypothetical protein
79Pmu_03530346765..346953 [-], 189hypothetical protein
80ssb1347260..347712 [-], 453single-stranded DNA-binding protein
81Pmu_03550348068..348553 [-], 486hypothetical protein
82Pmu_03560348562..349320 [-], 759integrating conjugative element protein, PFL family
83Pmu_03570349499..350689 [-], 1191hypothetical protein
84Pmu_03580350848..351405 [-], 558hypothetical protein
85Pmu_03590351398..353044 [-], 1647integrating conjugative element ParB family protein, PFGI-1 class
86dnaB1353053..354339 [-], 1287replicative DNA helicase
87Pmu_03610354426..355250 [-], 825chromosome partitioning ATPase
88Pmu_03640355836..356327 [+], 492heme iron utilization protein-like protein
89Pmu_03650356342..356857 [+], 516heme iron utilization protein, pyridoxamine 5'-phosphate domain-type
90hgbA356899..359802 [+], 2904hemoglobin-binding protein A
91rpsO359969..360238 [+], 27030S ribosomal protein S15
flank Flanking regions

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
(1) Michael GB; Kadlec K; Sweeney MT; Brzuszkiewicz E; Liesegang H; Daniel R; Murray RW; Watts JL; Schwarz S (2012). ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: structure and transfer. J Antimicrob Chemother. 67(1):91-100. [PudMed:22001176] experimental
(2) Michael GB; Kadlec K; Sweeney MT; Brzuszkiewicz E; Liesegang H; Daniel R; Murray RW; Watts JL; Schwarz S (2012). ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: analysis of the regions that comprise 12 antimicrobial resistance genes. J Antimicrob Chemother. 67(1):84-90. [PudMed:22001175] experimental
experimental experimental literature