T4SS ID120
StrainEscherichia coli plasmid pEC_L8 (NC_014384)
Repliconplasmid pEC_L8 [Browse all T4SS(s) in this replicon]
ClassificationType IVA; Type F
sequence This T4SS has been mentioned in a genome sequencing report.

T4SS components

The information of T4SS components from NC_014384
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1pECL8_p044 (psiB)41063..41500 [+], 438302595506plasmid SOS inhibition protein B 
2pECL8_p045 (psiA)41497..42216 [+], 720302595507plasmid SOS inhibition protein A 
3pECL8_p046 (mok)42228..42458 [-], 231302595508modulator of Hok protein 
4pECL8_p04843008..43232 [-], 225302595509hypothetical protein 
5pECL8_p04943276..43509 [-], 234302595510hypothetical protein 
6pECL8_p050 (yubP)44921..45073 [+], 153302595511hypothetical protein 
7pECL8_p051 (geneX)45099..45776 [-], 678302595512X-polypeptide  Orf169_F
8pECL8_p052 (traM)46032..46415 [+], 384302595513mating signal  TraM_Faccesspry protein
9pECL8_p053 (traJ)46549..47295 [+], 747302595514regulation protein 
10pECL8_p054 (traY)47389..47616 [+], 228302595515OriT nicking  TraY_F
11pECL8_p055 (traA)47626..48012 [+], 387302595516F pilin subunit  TraA_F
12pECL8_p056 (traL)48014..48328 [+], 315302595517F pilin assembly  TraL_F
13pECL8_p057 (traE)48350..48916 [+], 567302595518F pilin assembly  TraE_F
14pECL8_p058 (traK)48882..49631 [+], 750302595519F pilin assembly  TraK_F
15pECL8_p059 (traB)49628..51058 [+], 1431302595520F pilin assembly  TraB_F
16pECL8_p060 (traP)51006..51632 [+], 627302595521conjugal transfer protein 
17pECL8_p061 (trbD)51529..51939 [+], 411302595522conjugal transfer protein 
18pECL8_p062 (trbG)51917..52183 [+], 267302595523conjugal transfer protein 
19pECL8_p064 (traR)52830..53051 [+], 222302595524conjugal transfer protein 
20pECL8_p066 (trbI)55820..56221 [+], 402302595525conjugal transfer protein 
21pECL8_p067 (traW)56104..56850 [+], 747302595526F pilus assembly  TraW_F
22pECL8_p068 (traU)56820..57839 [+], 1020302595527F pilin assembly  TraU_F
23pECL8_p069 (yfdA)57863..58174 [+], 312302595528hypothetical protein 
24pECL8_p070 (trbC)58171..58821 [+], 651302595529F pilin assembly  TrbC_F
25pECL8_p071 (traN)58818..60626 [+], 1809302595530type IV secretion-like conjugative transfer system mating pair stabilization protein  TraN_F
26pECL8_p072 (trbE)60650..60910 [+], 261302595531conjugal transfer protein 
27pECL8_p074 (trbA)61662..62000 [+], 339302595532conjugative transfer protein 
28pECL8_p075 (traQ)62127..62411 [+], 285302595533conjugal transfer protein  TraQ_F
29pECL8_p076 (trbB)62398..62943 [+], 546302595534F pilin assembly periplasmic protein 
30pECL8_p077 (trbJ)62792..63214 [+], 423302595535conjugal transfer protein 
31pECL8_p078 (trbF)63162..63587 [+], 426302595536conjugal transfer protein 
32pECL8_p079 (traH)63568..64947 [+], 1380302595537F pilin assembly  TraH_F
33pECL8_p080 (traG)64944..67763 [+], 2820302595538type IV secretion-like conjugative transfer system protein  TraG_F
34pECL8_p081 (traS)67782..68279 [+], 498302595539conjugal transfer protein 
35pECL8_p082 (traT)68161..69042 [+], 882302595540conjugal transfer surface exclusion protein 
36pECL8_p083 (traD)69268..71520 [+], 2253302595541coupling protein  TraD_F
37pECL8_p084 (traI)71517..76790 [+], 5274302595542nickase/helicase  TraI_F
38pECL8_p085 (traX)76339..77556 [+], 1218302595543F pilin acetylation protein  TraX_F
39pECL8_p086 (finO)78554..79138 [+], 585302595544fertility inhibition protein 
40pECL8_p087 (yigA)79228..79479 [+], 252302595545hypothetical protein 
41pECL8_p089 (hhA)80201..80440 [+], 240302595546hypothetical protein 
42pECL8_p090 (yihA)80478..81068 [+], 591302595547hypothetical protein 
43pECL8_p091 (repA2)81308..81568 [+], 261302595548negative regulator of repA1 expression in FII replicon 
44pECL8_p092 (repA3)81653..81847 [+], 195302595549regulator of repA1 expression in FII replicon 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

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Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1TraM (302595513)NDTraIFor transfer initiation, traM mediates signalling between the relaxosome and transferosome.(1) PubMed: 22788760
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Wong JJ; Lu J; Glover JN (2012). Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. [PudMed:22788760]
(1) Smet A; Van Nieuwerburgh F; Vandekerckhove TT; Martel A; Deforce D; Butaye P; Haesebrouck F (2010). Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences. PLoS One. 5(6):e11202. [PudMed:20585456]