T4SS ID614
StrainEnterococcus faecalis V583
Repliconplasmid pTEF2 [Browse all T4SS(s) in this replicon]
ClassificationType IVD; Type GP
sequence This T4SS has been mentioned in a genome sequencing report.

T4SS components

The information of T4SS components from NC_004671
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1EF_B0004 (traC-2)2486..4075 [+], 159029377898TraC protein 
2EF_B00054101..5042 [-], 94229377899Cro/CI family transcriptional regulator 
3EF_B000515255..5332 [+], 7829377900hypothetical protein 
4EF_B00075822..6202 [+], 38129377901pheromone-responsive regulatory protein R 
5EF_B00086776..6964 [+], 18929377902hypothetical protein 
6EF_B00096968..7081 [+], 11429377903hypothetical protein 
7EF_B0010 (prgA)7092..9767 [+], 267629377904surface exclusion protein PrgA  PrgA
8EF_B0011 (prgB)9960..13877 [+], 391829377905aggregation substance PrgB  PrgBaccesspry protein
9EF_B0012 (prgC)14323..15180 [+], 85829377906surface protein PrgC  PrgC
10EF_B001315222..16121 [+], 90029377907hypothetical protein  PrgD
11EF_B001416141..16575 [+], 43529377908hypothetical protein 
12EF_B001516622..16849 [+], 22829377909hypothetical protein  PrgF
13EF_B001616863..17159 [+], 29729377910hypothetical protein 
14EF_B001717174..17974 [+], 80129377911hypothetical protein  PrgH
15EF_B001817976..18329 [+], 35429377912hypothetical protein  PrgI
16EF_B001918436..20673 [+], 223829377913hypothetical protein  PrgJ
17EF_B002020685..23300 [+], 261629377914hypothetical protein  PrgK
18EF_B002123324..23950 [+], 62729377915hypothetical protein  PrgL
19EF_B002223928..24182 [+], 25529377916hypothetical protein 
20EF_B002324166..24774 [+], 60929377917hypothetical protein 
21EF_B002424931..25419 [+], 48929377918hypothetical protein 
22EF_B002525416..27248 [+], 183329377919TraG family protein  PcfC
23EF_B002627298..29457 [+], 216029377920hypothetical protein 
24EF_B002729490..29762 [+], 27329377921LtrD-related protein, putative 
25EF_B002930008..30364 [+], 35729377922hypothetical protein 
26EF_B003030365..32050 [+], 168629377923relaxase 
27EF_B003132104..32433 [+], 33029377924hypothetical protein  PcfH
28EF_B003232757..32924 [+], 16829377925hypothetical protein 
29EF_B003332950..34293 [-], 134429377926hypothetical protein 
30EF_B003434284..35060 [-], 77729377927hypothetical protein 
31EF_B003535203..35751 [-], 54929377928hypothetical protein 
32EF_B003635775..35948 [-], 17429377929hypothetical protein 
33EF_B003735951..36136 [-], 18629377930hypothetical protein 
34EF_B003836142..36591 [-], 45029377931hypothetical protein 
35EF_B004036878..37078 [+], 20129377932hypothetical protein 
36EF_B004137179..37409 [-], 23129377933hypothetical protein 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1PrgB (29377905)ND-Pheromonedependent overproduction of PrgB induces formation of intercellular aggregates. However, upon overproduction, PrgB confers toxicity on E. faecalis donors by a mechanism dependent on extracellular DNA.(1) PubMed: 25431047
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Bhatty M et al. (2015). Enterococcus faecalis pCF10-encoded surface proteins PrgA, PrgB (aggregation substance) and PrgC contribute to plasmid transfer, biofilm formation and virulence. Mol Microbiol. 95(4):660-77. [PudMed:25431047] experimental
(1) Paulsen IT; Banerjei L; Myers GS; Nelson KE; Seshadri R; Read TD; Fouts DE; Eisen JA; Gill SR; Heidelberg JF; Tettelin H; Dodson RJ; Umayam L; Brinkac L; Beanan M; Daugherty S; DeBoy RT; Durkin S; Kolonay J; Madupu R; Nelson W; Vamathevan J; Tran B; Upton J; Hansen T; Shetty J; Khouri H; Utterback T; Radune D; Ketchum KA; Dougherty BA; Fraser CM (2003). Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis. Science. 299(5615):2071-4. [PudMed:12663927]