T4SS ID615
StrainEnterococcus faecalis plasmid pCF10 (NC_006827)
Repliconplasmid pCF10 [Browse all T4SS(s) in this replicon]
ClassificationType IVD; Type GP
experimental Experimental investigation has been performed on this T4SS.
structure Information of structure: structurePcfF, PrgB, PrgB bound to DNA

T4SS components

The information of T4SS components from NC_006827
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1pCF10_09 (prgY)5843..6997 [+], 115558616131PrgY 
2pCF10_10 (prgX)7030..7983 [-], 95458616133PrgX 
3pCF10_11 (prgQ)8192..8263 [+], 7258616132PrgQ 
4pCF10_12 (prgR)8745..9125 [+], 381113706805PrgR 
5pCF10_13 (prgS)9125..9397 [+], 27358616086PrgS 
6pCF10_14 (prgT)9699..9887 [+], 18958616137PrgT 
7pCF10_15 (prgA)10015..12690 [+], 267658616134PrgA  PrgA
8pCF10_16 (prgB)12883..16800 [+], 391858616135PrgB  PrgBaccesspry protein
9pCF10_17 (prgU)16862..17218 [+], 35758616087PrgU 
10pCF10_18 (prgC)17246..18103 [+], 85858616136PrgC  PrgC
11pCF10_19 (prgD)18145..19044 [+], 90058616088PrgD  PrgD
12pCF10_20 (prgE)19064..19498 [+], 43558616089PrgE 
13pCF10_21 (prgF)19545..19772 [+], 22858616090PrgF  PrgF
14pCF10_22 (prgG)19786..20082 [+], 29758616091PrgG 
15pCF10_23 (prgH)20097..20897 [+], 80158616084PrgH  PrgH
16pCF10_24 (prgI)20899..21252 [+], 35458616092PrgI  PrgI
17pCF10_25 (prgJ)21359..23596 [+], 223858616093PrgJ  PrgJ
18pCF10_26 (prgK)23608..26223 [+], 261658616094PrgK  PrgK
19pCF10_27 (prgL)26247..26873 [+], 62758616095PrgL  PrgL
20pCF10_28 (prgM)26860..27105 [+], 24658616096PrgM 
21pCF10_29 (pcfA)27089..27697 [+], 60958616097PcfA 
22pCF10_30 (pcfB)27856..28341 [+], 486113706806PcfB 
23pCF10_31 (pcfC)28341..30170 [+], 183058616099PcfC  PcfC
24pCF10_32 (pcfD)30221..32380 [+], 216058616100PcfD 
25pCF10_33 (pcfE)32413..32685 [+], 27358616101PcfE 
26pCF10_34 (pcfF)32931..33287 [+], 35758616102PcfF  accesspry protein
27pCF10_35 (pcfG)33288..34973 [+], 168658616103PcfG 
28pCF10_36 (pcfH)35006..35356 [+], 35158616104PcfH  PcfH
29pCF10_37 (pcfI)35680..35847 [+], 16858616105PcfI 
30pCF10_38 (pcfJ)35873..37210 [-], 133858616106PcfJ 
31pCF10_39 (pcfK)37207..37983 [-], 77758616107PcfK 
32pCF10_40 (pcfL)38126..38674 [-], 54958616108PcfL 
33pCF10_41 (pcfM)38698..38889 [-], 19258616126PcfM 
34pCF10_42 (pcfN)38874..39059 [-], 18658616109PcfN 
35pCF10_43 (pcfO)39065..39514 [-], 45058616110PcfO 
36pCF10_44 (pcfP)39801..40001 [+], 20158616111PcfP 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes
The information on structure of this T4ss

1PcfF6QEQ PDB (6QEQ)PcfF from Enterococcus faecalis pCF10.(1) PubMed: 31134011
2PrgB6EVU PDB (6EVU)Adhesin domain of PrgB from Enterococcus faecalis.(2) PubMed: 29723434
3PrgB bound to DNA6GED PDB (6GED)Adhesin domain of PrgB from Enterococcus faecalis bound to DNA.(3) PubMed: 29723434
(1) Rehman S et al. (2019). Enterococcal PcfF Is a Ribbon-Helix-Helix Protein That Recruits the Relaxase PcfG Through Binding and Bending of the oriT Sequence. Front Microbiol. 10:958. [PudMed:31134011] experimental
(2) Schmitt A et al. (2018). PrgB promotes aggregation, biofilm formation, and conjugation through DNA binding and compaction. Mol Microbiol. 109(3):291-305. [PudMed:29723434] experimental
(3) Schmitt A et al. (2018). PrgB promotes aggregation, biofilm formation, and conjugation through DNA binding and compaction. Mol Microbiol. 109(3):291-305. [PudMed:29723434] experimental
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1PcfF (58616102)ND (interacts with PcfC T4CP)PcfGPcfF interacts with PcfC and PcfG, spatially positions relaxosome or transfer intermediate near the T4CP.(1) PubMed: 18811729
2PrgB (58616135)ND-Pheromonedependent overproduction of PrgB induces formation of intercellular aggregates. However, upon overproduction, PrgB confers toxicity on E. faecalis donors by a mechanism dependent on extracellular DNA.(2) PubMed: 25431047
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Al-Khodor S; Price CT; Habyarimana F; Kalia A; Abu Kwaik Y (2008). A Dot/Icm-translocated ankyrin protein of Legionella pneumophila is required for intracellular proliferation within human macrophages and protozoa. Mol Microbiol. 70(4):908-23. [PudMed:18811729] experimental
(2) Bhatty M et al. (2015). Enterococcus faecalis pCF10-encoded surface proteins PrgA, PrgB (aggregation substance) and PrgC contribute to plasmid transfer, biofilm formation and virulence. Mol Microbiol. 95(4):660-77. [PudMed:25431047] experimental
(1) Li F; Alvarez-Martinez C; Chen Y; Choi KJ; Yeo HJ; Christie PJ (2012). Enterococcus faecalis PrgJ, a VirB4-like ATPase, Mediates pCF10 Conjugative Transfer Through Substrate Binding. J Bacteriol. . [PudMed:22636769] experimental
(2) Chen Y; Zhang X; Manias D; Yeo HJ; Dunny GM; Christie PJ (2008). Enterococcus faecalis PcfC, a spatially localized substrate receptor for type IV secretion of the pCF10 transfer intermediate. J Bacteriol. 190(10):3632-45. [PudMed:18326569] experimental
experimental This literature contains experimental investigation