I. Summary

SecReT6 ID	105
Strain	Burkholderia sp. 383
Replicon	chromosome 2 [Browse all T6SS(s) in this replicon]
Accession	NC_007511
Location	1082518..1121246
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	2	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

14

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	Bcep18194_B0965	1077810..1079012 [+], 1203	78061815	D-isomer specific 2-hydroxyacid dehydrogenase
2	Bcep18194_B0966	1079053..1080192 [-], 1140	78061816	patatin
3	Bcep18194_B0967	1080404..1080865 [-], 462	78061817	hypothetical protein
4	Bcep18194_B0968	1080960..1081292 [-], 333	78061818	plastocyanin
5	Bcep18194_B0969	1081318..1082262 [-], 945	78061819	metallophosphoesterase
6	Bcep18194_B0970	1082518..1084107 [+], 1590	78061820	phosphate transporter
7	Bcep18194_B0971	1084189..1085940 [+], 1752	78061821	ABC anion transporter, fused inner membrane subunits
8	Bcep18194_B0972	1085952..1087298 [+], 1347	78061822	nitrate/sulfonate/bicarbonate ABC transporter ATPase
9	Bcep18194_B0973	1087349..1088467 [-], 1119	78061823	ImpA-like protein	TssA
10	Bcep18194_B0974	1088464..1089519 [-], 1056	78061824	hypothetical protein	TssG
11	Bcep18194_B0975	1089519..1091408 [-], 1890	78061825	hypothetical protein	TssF
12	Bcep18194_B0976	1091446..1092018 [-], 573	78061826	hypothetical protein	TssE
13	Bcep18194_B0977	1092011..1092865 [-], 855	78061827	virulence protein, SciE type	TagJ
14	Bcep18194_B0978	1092862..1093512 [-], 651	78061828	hypothetical protein
15	Bcep18194_B0979	1094050..1096731 [+], 2682	78061829	ATPase AAA	TssH
16	Bcep18194_B0980	1096768..1097307 [+], 540	78061830	hypothetical protein	TssB
17	Bcep18194_B0981	1097335..1098828 [+], 1494	78061831	hypothetical protein	TssC
18	Bcep18194_B0982	1098922..1099407 [+], 486	78061832	hypothetical protein	TssD
19	Bcep18194_B0983	1099487..1099990 [+], 504	78061833	hypothetical protein	TssJ
20	Bcep18194_B0984	1100022..1101368 [+], 1347	78061834	hypothetical protein	TssK
21	Bcep18194_B0985	1101550..1104081 [+], 2532	78061835	Rhs element Vgr protein	TssI
22	Bcep18194_B0986	1104053..1104976 [+], 924	78061836	hypothetical protein
23	Bcep18194_B0987	1104973..1105494 [+], 522	78061837	hypothetical protein
24	Bcep18194_B0988	1105593..1106120 [+], 528	78061838	hypothetical protein
25	Bcep18194_B0989	1106133..1108508 [+], 2376	78061839	hypothetical protein
26	Bcep18194_B0990	1108534..1108815 [+], 282	78061840	hypothetical protein	PAAR
27	Bcep18194_B0991	1108818..1110122 [+], 1305	78061841	hypothetical protein	TssL
28	Bcep18194_B0992	1110148..1114257 [+], 4110	78061842	hypothetical protein	TssM
29	Bcep18194_B0993	1114275..1115489 [+], 1215	78061843	OmpA/MotB family outer membrane protein	TssL
30	Bcep18194_B0994	1115779..1116720 [+], 942	78061844	hypothetical protein
31	Bcep18194_B0995	1116699..1117577 [-], 879	78061845	RpiR family transcriptional regulator
32	Bcep18194_B0996	1117941..1119692 [+], 1752	78061846	gamma-glutamyltransferase 1
33	Bcep18194_B0997	1119870..1121246 [+], 1377	78061847	Sodium/dicarboxylate symporter
34	Bcep18194_B0998	1121276..1121674 [-], 399	78061848	hypothetical protein
35	Bcep18194_B0999	1121719..1123011 [-], 1293	78061849	FAD-dependent pyridine nucleotide-disulfide oxidoreductase
36	Bcep18194_B1000	1123200..1123814 [+], 615	78061850	TetR family transcriptional regulator
37	Bcep18194_B1001	1123839..1124750 [-], 912	78061851	LysR family transcriptional regulator
38	Bcep18194_B1002	1124859..1125620 [+], 762	78061852	Short-chain dehydrogenase/reductase SDR
39	Bcep18194_B1003	1125676..1126155 [-], 480	78061853	MerR family transcriptional regulator

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

SciK

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	Bcep18194_B0982	1098922..1099407 [+], 486	78061832	hypothetical protein	SciK

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Proteins        Genes

N/A

(1) Boyer F et al. (2009). Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 10:104. [PudMed:19284603]

This literature contains bioinformatics investigation