I. Summary
SecReT6 ID113 experimental
StrainBurkholderia thailandensis E264
Repliconchromosome II [Browse all T6SS(s) in this replicon]
CommentVirulence in mouse acute pneumonia model of melioidosis; mononuclear giant cell formation and intercellular spread in cultured cells
Functionanti-eukaryotic; virulence
T6SS TypeType i; subtype i1
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1BTH_II0850992960..993415 [+], 45683718111type III secretion chaperone BicP 
2BTH_II0851993650..994321 [+], 67283716526DNA-binding response regulator 
3BTH_II0852994546..994989 [+], 44483717070hypothetical protein 
4BTH_II0853995098..996285 [+], 118883717752AraC family transcriptional regulator 
5BTH_II0854996276..997595 [-], 132083717089ubiquitin-specific proteinase 31 
6BTH_II0855997685..1001575 [-], 389183716079lipoprotein  TssM
7BTH_II08561001572..1002252 [-], 68183716722hypothetical protein  TssL
8BTH_II08571002249..1003643 [-], 139583716919hypothetical protein  TssK
9BTH_II08581003676..1004482 [-], 80783717233hypothetical protein  TssJ
10BTH_II08591004492..1004851 [-], 36083716426hypothetical protein 
11BTH_II08601004913..1005707 [-], 79583716769lipoprotein 
12BTH_II08611005686..1006750 [-], 106583717467pentapeptide repeat-containing protein 
13BTH_II08621006768..1009386 [-], 261983717943pentapeptide repeat-containing protein 
14BTH_II08631009425..1012463 [-], 303983717090Rhs element Vgr protein  TssI
15BTH_II08641012489..1015365 [-], 287783717809ATP-dependent Clp protease, ATP-binding subunit ClpB  TssH
16BTH_II08651015352..1016407 [-], 105683717117hypothetical protein  TssG
17BTH_II08661016371..1018113 [-], 174383716626hypothetical protein  TssF
18BTH_II08671018142..1018618 [-], 47783717481hypothetical protein  TssE
19BTH_II08681018597..1019085 [-], 48983716550hcp protein  TssD
20BTH_II08691019268..1020794 [-], 152783716783hypothetical protein  TssC
21BTH_II08701020791..1021285 [-], 49583717451hypothetical protein  TssB
22BTH_II08711021668..1023368 [+], 170183717576sensor histidine kinase  VirA
23BTH_II08721023365..1024099 [+], 73583718212DNA-binding response regulator  VirG
24BTH_II08731024090..1025886 [+], 179783716378ImpA-like N-terminal family protein  TssA
25BTH_II08741026177..1026500 [-], 32483717096hypothetical protein 
26BTH_II08751026584..1028185 [-], 160283716561Hep_Hag family protein 
27BTH_II08761028324..1029595 [-], 127283717327hypothetical protein 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
BTH_II0868 , BMAA0737, BTH_II1883

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1BTH_II08681018597..1019085 [-], 48983716550hcp protein BTH_II0868
2BTH_II08631009425..1012463 [-], 303983717090Rhs element Vgr protein BMAA0737
3BTH_II18832281342..2281767 [-], 42683718045hypothetical protein BTH_II1883
Data from experimental literature

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Proteins        Genes
The information on regulator interaction.

#homolog effector locus tagRegulator wayFunctionRegulate targetDescription
1BTH_I1281 (OxyR)Transcriptional-BTH_RS09735 (T6SS-4) Promotor,?536 to ?186 relative to the ATG start codonOxyR in chromosome I negatively regulates the expression of T6SS-4 in chromosome II by specifically recognizing an operator within the T6SS-4 promoter.
2bth_I0691 (Zur)Transcription regulator-BTH_RS09735 (T6SS-4) Promotor,?541 to ?1 relative to the ATG start codonbth_I0691 (Zur) in chromosome I directly represses expression of T6SS4 in chromosome II by interaction of T6SS4 promoter .
(1) Schwarz S et al. (2014). VgrG-5 is a Burkholderia type VI secretion exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 82(4):1445-52. [PudMed:24452686] experimental
(2) Fisher NA et al. (2012). The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 12:117. [PudMed:22892068]
(3) Russell AB et al. (2012). A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 11(5):538-49. [PudMed:22607806] experimental in_silico
(4) Schwarz S et al. (2010). Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 6(8):e1001068. [PudMed:20865170] experimental
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation