I. Summary
SecReT6 ID113 experimental
StrainBurkholderia thailandensis E264
Repliconchromosome II [Browse all T6SS(s) in this replicon]
CommentVirulence in mouse acute pneumonia model of melioidosis; mononuclear giant cell formation and intercellular spread in cultured cells
Functionanti-eukaryotic; virulence
T6SS TypeType i; subtype i1
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1BTH_RS04415 (sicP)992960..993415 [+], 456497581981chaperone SicP 
2BTH_RS04420 (bprB)993650..994321 [+], 672499720317two-component system response regulator BprB 
3BTH_RS04425994546..994989 [+], 444497581983hypothetical protein 
4BTH_RS04430995224..996285 [+], 1062497581984helix-turn-helix domain-containing protein 
5BTH_RS04435996276..997382 [-], 1107644828721Ig-like domain-containing protein 
6BTH_RS04440997685..1001575 [-], 3891497581989type VI secretion system protein ImpL  TssM
7BTH_RS044451001572..1002249 [-], 678644828725DotU family type IV/VI secretion system protein 
8BTH_RS04450 (tssK)1002249..1003643 [-], 1395497581992type VI secretion system baseplate subunit TssK  TssK
9BTH_RS04455 (tssJ)1003676..1004482 [-], 807499720319type VI secretion system lipoprotein TssJ  TssJ
10BTH_RS044601004492..1004884 [-], 393497581996DUF4150 domain-containing protein 
11BTH_RS044651004913..1005689 [-], 777746292543DUF3540 domain-containing protein 
12BTH_RS044701005686..1006750 [-], 1065497582000pentapeptide repeat-containing protein 
13BTH_RS044751006768..1009386 [-], 2619497582002pentapeptide repeat-containing protein 
14BTH_RS04480 (tssI)1009425..1012463 [-], 3039497582003type VI secretion system tip protein VgrG  TssI
15BTH_RS04485 (tssH)1012489..1015365 [-], 2877497582005type VI secretion system ATPase TssH  TssH
16BTH_RS04490 (tssG)1015352..1016383 [-], 1032497582007type VI secretion system baseplate subunit TssG 
17BTH_RS04495 (tssF)1016371..1018113 [-], 1743497582009type VI secretion system baseplate subunit TssF  TssF
18BTH_RS04500 (tssE)1018142..1018618 [-], 477497582010type VI secretion system baseplate subunit TssE  TssE
19BTH_RS045051018597..1019085 [-], 489497582011Hcp family type VI secretion system effector  TssD
20BTH_RS04510 (tssC)1019268..1020794 [-], 1527497582013type VI secretion system contractile sheath large subunit  TssC
21BTH_RS04515 (tssB)1020791..1021285 [-], 495497582014type VI secretion system contractile sheath small subunit  TssB
22BTH_RS045201021545..1023368 [+], 1824497582015MASE1 domain-containing protein 
23BTH_RS045251023365..1024099 [+], 735497593277response regulator transcription factor  VirG
24BTH_RS04530 (tssA)1024090..1025886 [+], 1797497582018type VI secretion system protein TssA  TssA
25BTH_RS045351026177..1026533 [-], 357644828750hypothetical protein 
26BTH_RS045401026584..1027147 [-], 564497582021YadA-like family protein 
27BTH_RS297951027413..1027817 [+], 405497582022hypothetical protein 
28BTH_RS045451028324..1029649 [-], 1326644828755autotransporter strand-loop-strand O-heptosyltransferase 
29BTH_RS045501029806..1030831 [-], 1026497582025N-acetylmuramoyl-L-alanine amidase 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
BTH_II0868 , BMAA0737, BTH_II1883

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

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Proteins        Genes
The information on regulator interaction.

#homolog effector locus tagRegulator wayFunctionRegulate targetDescription
1BTH_I1281 (OxyR)Transcriptional-BTH_RS09735 (T6SS-4) Promotor,?536 to ?186 relative to the ATG start codonOxyR in chromosome I negatively regulates the expression of T6SS-4 in chromosome II by specifically recognizing an operator within the T6SS-4 promoter.
2bth_I0691 (Zur)Transcription regulator-BTH_RS09735 (T6SS-4) Promotor,?541 to ?1 relative to the ATG start codonbth_I0691 (Zur) in chromosome I directly represses expression of T6SS4 in chromosome II by interaction of T6SS4 promoter .
(1) Schwarz S et al. (2014). VgrG-5 is a Burkholderia type VI secretion exported protein required for multinucleated giant cell formation and virulence. Infect Immun. 82(4):1445-52. [PudMed:24452686] experimental
(2) Fisher NA et al. (2012). The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 12:117. [PudMed:22892068]
(3) Russell AB et al. (2012). A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 11(5):538-49. [PudMed:22607806] experimental in_silico
(4) Schwarz S et al. (2010). Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 6(8):e1001068. [PudMed:20865170] experimental
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation