I. Summary

SecReT6 ID	114
Strain	Burkholderia thailandensis E264
Replicon	chromosome II [Browse all T6SS(s) in this replicon]
Accession	NC_007650
Location	2282208..2308569
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	2	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	BTH_RS09625	2277721..2278998 [-], 1278	497582378	glycosyltransferase
2	BTH_RS09630	2279278..2280177 [-], 900	497582376	alpha/beta hydrolase
3	BTH_RS09635	2280750..2281025 [-], 276	497593796	hypothetical protein
4	BTH_RS09640	2281342..2281767 [-], 426	499720685	hypothetical protein
5	BTH_RS09645	2281790..2282149 [-], 360	497579572	hypothetical protein
6	BTH_RS09650 (tssM)	2282208..2285702 [-], 3495	497579573	type VI secretion system membrane subunit TssM
7	BTH_RS09655 (icmH)	2285699..2287426 [-], 1728	497579574	type IVB secretion system protein IcmH/DotU
8	BTH_RS09660 (tssK)	2287509..2288870 [-], 1362	497579575	type VI secretion system baseplate subunit TssK
9	BTH_RS09665	2288867..2289460 [-], 594	497579576	type VI secretion lipoprotein TssJ
10	BTH_RS09670	2289466..2289855 [-], 390	497579577	DUF4150 domain-containing protein
11	BTH_RS09675	2289895..2290611 [-], 717	497579579	DUF3540 domain-containing protein
12	BTH_RS09680	2290614..2291684 [-], 1071	497579581	pentapeptide repeat-containing protein
13	BTH_RS09685	2291684..2293900 [-], 2217	497579582	pentapeptide repeat-containing protein
14	BTH_RS09690 (vgrG)	2293904..2296192 [-], 2289	497579584	type VI secretion system tip protein VgrG
15	BTH_RS09695 (vgrG)	2296361..2298652 [-], 2292	499720689	type VI secretion system tip protein VgrG
16	BTH_RS09700 (tssH)	2298643..2301486 [-], 2844	497579587	type VI secretion system ATPase TssH
17	BTH_RS09705 (tssG)	2301489..2302478 [-], 990	497579588	type VI secretion system baseplate subunit TssG
18	BTH_RS09710 (tssF)	2302475..2304337 [-], 1863	497586827	type VI secretion system baseplate subunit TssF
19	BTH_RS09715	2304351..2304782 [-], 432	497586828	GPW/gp25 family protein
20	BTH_RS09720	2304861..2305388 [-], 528	497586829	type VI secretion system tube protein Hcp
21	BTH_RS09725 (tssC)	2305532..2307037 [-], 1506	497586830	type VI secretion system contractile sheath large subunit
22	BTH_RS09730 (tssB)	2307040..2307591 [-], 552	497586831	type VI secretion system contractile sheath small subunit
23	BTH_RS09735	2307625..2308569 [-], 945	497593800	type VI secretion system ImpA family N-terminal domain-containing protein
24	BTH_RS09740	2310190..2311704 [-], 1515	497579594	acetyl-CoA hydrolase/transferase family protein
25	BTH_RS09745	2312067..2312762 [+], 696	497579596	CDP-alcohol phosphatidyltransferase family protein
26	BTH_RS09750	2312759..2313532 [+], 774	497579598	2OG-Fe(II) oxygenase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

YPK_3563

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog

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Proteins        Genes

N/A

(1) Russell AB et al. (2012). A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 11(5):538-49. [PudMed:22607806]

(2) Schwarz S et al. (2010). Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 6(8):e1001068. [PudMed:20865170]

This literature contains experimental investigation

This literature contains bioinformatics investigation