I. Summary
SecReT6 ID115 experimental
StrainBurkholderia thailandensis E264
Repliconchromosome I [Browse all T6SS(s) in this replicon]
CommentProliferation in mixed culture with several Gram-negative bacteria, resistance to Pseudomonas putida-induced stasis and persistence in mixed biofilms with P. putida
Functionanti-bacterial;effector translocation;adherence
T6SS TypeType i; subtype i4b
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1BTH_I29503392434..3393873 [+], 144083719927TolC family type I secretion outer membrane protein 
2BTH_I29513394070..3395035 [-], 96683720694hypothetical protein 
3BTH_I29523395047..3396012 [+], 96683721600hypothetical protein 
4BTH_I29533396175..3396273 [+], 9983718986hypothetical protein 
5BTH_I29543396298..3400203 [+], 390683721202ImcF-like family protein  TssM
6BTH_I29553400200..3401189 [+], 99083718465hypothetical protein 
7BTH_I29563401194..3402129 [+], 93683718754OmpA domain-containing protein  TagL
8BTH_I29573402329..3403438 [-], 111083718946ImpA-like N-terminal family protein  TssA
9BTH_I29583403545..3406208 [-], 266483720142ClpA/B type protease  TssH
10BTH_I29593406242..3407342 [-], 110183720854hypothetical protein  TssG
11BTH_I29603407306..3409144 [-], 183983719151hypothetical protein  TssF
12BTH_I29613409225..3409707 [-], 48383719917hypothetical protein  TssE
13BTH_I29623409765..3410268 [-], 50483721066hypothetical protein  TssD
14BTH_I29633410341..3411831 [-], 149183721364hypothetical protein  TssC
15BTH_I29643411848..3412366 [-], 51983719260EvpA  TssB
16BTH_I29653412403..3413092 [-], 69083720413lipoprotein 
17BTH_I29663413202..3414080 [+], 87983718738hypothetical protein  TssJ
18BTH_I29673414189..3415535 [+], 134783719347hypothetical protein  TssK
19BTH_I29683415532..3416317 [+], 78683720562ompA family protein  TssL
20BTH_I29713416885..3417079 [+], 19583719226hypothetical protein 
21BTH_I2973 (sspB)3417661..3418173 [-], 51383718527ClpXP protease specificity-enhancing factor 
22BTH_I29743418243..3418854 [-], 61283721108stringent starvation protein A 
23BTH_I29753418947..3419705 [-], 75983720309ubiquinol-cytochrome c reductase, cytochrome c1 
24BTH_I29763419728..3421110 [-], 138383719787ubiquinol-cytochrome c reductase, cytochrome b 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
BTH_II0090 , BTH_I3225, BTH_II0310, BTH_I2693, BTH_I3225, BTH_I2705, BTH_I2698, BTH_I3226, BTH_I2697, BTH_I2691, BTH_I0068 (Tae2), BTH_I2698 (Tle1), BCAL0343, BTH_I1830

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1BTH_II0090100528..102714 [+], 218783716109hypothetical protein BTH_II0090
2BTH_II008997680..100460 [+], 278183716745Rhs element Vgr protein BTH_I3225
3BTH_II0310379615..380307 [-], 69383716914antifungal protein BTH_II0310
4BTH_I26933081345..3084173 [-], 282983718482Rhs element Vgr protein BTH_I2693
5BTH_I32253671318..3674098 [+], 278183718625Rhs element Vgr protein BTH_I3225
6BTH_I27053097448..3100246 [-], 279983718826Rhs element Vgr protein BTH_I2705
7BTH_I27013092917..3095325 [-], 240983719397hypothetical protein BTH_I2698
8BTH_I32263674166..3676352 [+], 218783719543hypothetical protein BTH_I3226
9BTH_I26973085793..3089128 [-], 333683720129Rhs element Vgr protein BTH_I2697
10BTH_I26913077299..3080322 [-], 302483720200hypothetical protein BTH_I2691
11BTH_I006873967..74407 [+], 44183720828hypothetical protein (Tae2)BTH_I0068 (Tae2)
12BTH_I26983089237..3091639 [-], 240383721061hypothetical protein (Tle1)BTH_I2698 (Tle1)
13BTH_I29623409765..3410268 [-], 50483721066hypothetical protein BCAL0343
14BTH_I18302056900..2057424 [-], 52583721528hypothetical protein BTH_I1830
Data from experimental literature

Download FASTA format files
Proteins        Genes

Homology Immunity protein list
BTH_I2700, BTH_I0069 (Tai2), BTH_I2700, BTH_I2699 (Tli1)

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1BTH_I27003092232..3092810 [-], 57983718861lipoprotein BTH_I2700
2BTH_I006974394..74789 [+], 39683719910lipoprotein (Tai2)BTH_I0069 (Tai2)
3BTH_I27033095918..3096661 [-], 74483720303hypothetical protein BTH_I2700
4BTH_I26993091636..3092235 [-], 60083720426hypothetical protein (Tli1)BTH_I2699 (Tli1)
Data from experimental literature

Download FASTA format files
Proteins        Genes
The information on regulator interaction.

#homolog effector locus tagRegulator wayFunctionRegulate targetDescription
1BTH_II1510 (BtaR1)QS+BTH_I2691/BTH_I2693-99/BTH_I2701-702QS activates nine genes for T6SS-1 substrates.
(1) Russell AB et al. (2013). Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 496(7446):508-12. [PudMed:23552891] experimental
(2) Fisher NA et al. (2012). The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 12:117. [PudMed:22892068]
(3) Russell AB et al. (2012). A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 11(5):538-49. [PudMed:22607806] experimental in_silico
(4) Schwarz S et al. (2010). Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 6(8):e1001068. [PudMed:20865170] experimental
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation