I. Summary

SecReT6 ID	121
Strain	Escherichia coli UTI89
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_007946
Location	3097390..3137091
Comment
Function	N/A
T6SS Type	Type i; subtype i2
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	2	1	1	1	0
Component	TssG	TssJ	TssK	TssM
Number	0	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	UTI89_C3176 (fucU)	3093393..3093815 [+], 423	91212173	protein of fucose operon
2	UTI89_C3177 (fucR)	3093864..3094604 [+], 741	91212174	DNA-binding transcriptional activator FucR
3	UTI89_C3178 (ygdE)	3094648..3095748 [-], 1101	91212175	RNA 2'-O-ribose methyltransferase
4	UTI89_C3179 (ygdD)	3095741..3096043 [-], 303	91212176	hypothetical protein
5	UTI89_C3180 (gcvA)	3096155..3097072 [-], 918	91212177	DNA-binding transcriptional activator GcvA
6	UTI89_C3181	3097390..3097683 [+], 294	91212178	hypothetical protein
7	UTI89_C3182	3097423..3097653 [-], 231	91212179	hypothetical protein
8	UTI89_C3183	3097806..3099047 [+], 1242	91212180	cysteine sulfinate desulfinase
9	UTI89_C3184 (ygdK)	3099047..3099490 [+], 444	91212181	hypothetical protein
10	UTI89_C3185 (ygdL)	3099541..3100347 [-], 807	91212182	hypothetical protein
11	UTI89_C3186 (mltA)	3100424..3101521 [-], 1098	162138382	murein transglycosylase A
12	UTI89_C3190	3102641..3103162 [+], 522	91212184	hypothetical protein	TssB
13	UTI89_C3191	3103215..3104759 [+], 1545	91212185	hypothetical protein	TssC
14	UTI89_C3192	3104777..3106114 [+], 1338	91212186	hypothetical protein	TssK
15	UTI89_C3193	3106111..3106776 [+], 666	91212187	hypothetical protein	TssL
16	UTI89_C3194	3106789..3108441 [+], 1653	91212188	hypothetical protein	TagL
17	UTI89_C3195	3108478..3109080 [-], 603	91212189	hypothetical protein
18	UTI89_C3196	3108499..3108990 [+], 492	91212190	secreted protein Hcp	TssD
19	UTI89_C3197	3109182..3111827 [+], 2646	91212191	ClpB protein	TssH
20	UTI89_C3198	3111839..3114319 [+], 2481	91212192	hypothetical protein	TssI
21	UTI89_C3199	3114339..3116108 [+], 1770	91212193	hypothetical protein	Tle4 (e)
22	UTI89_C3200	3116083..3116916 [+], 834	91212194	hypothetical protein	Tli4 (i)
23	UTI89_C3201	3116885..3117178 [+], 294	91212195	hypothetical protein
24	UTI89_C3202	3117642..3117902 [+], 261	91212196	hypothetical protein	PAAR
25	UTI89_C3203	3117829..3119040 [+], 1212	91212197	hypothetical protein
26	UTI89_C3204	3119050..3122403 [+], 3354	91212198	hypothetical protein	TssM
27	UTI89_C3205	3122369..3124006 [+], 1638	91212199	hypothetical protein	TssA
28	UTI89_C3206	3125577..3126218 [+], 642	91212200	hypothetical protein
29	UTI89_C3207	3126499..3126918 [+], 420	91212201	hypothetical protein
30	UTI89_C3208	3130072..3130608 [+], 537	91212202	hypothetical protein	TssJ
31	UTI89_C3209	3130612..3131040 [+], 429	91212203	hypothetical protein	TssE
32	UTI89_C3210	3131040..3132416 [+], 1377	91212204	hypothetical protein	TssA
33	UTI89_C3211	3131590..3131742 [-], 153	91212205	hypothetical protein
34	UTI89_C3212	3132718..3133674 [-], 957	91212206	2-hydroxyacid dehydrogenase
35	UTI89_C3213	3133737..3134333 [-], 597	91212207	phosphosugar isomerase
36	UTI89_C3214	3134336..3135511 [-], 1176	91212208	beta-cystathionase
37	UTI89_C3215	3135511..3137091 [-], 1581	91212209	PTS system maltose and glucose-specific transporter subunit IIABC
38	UTI89_C3216	3137123..3137992 [-], 870	91212210	antiterminator
39	UTI89_C3217	3138031..3138183 [+], 153	91212211	hypothetical protein
40	UTI89_C3218 (amiC)	3138193..3139536 [-], 1344	91212212	N-acetylmuramoyl-L-alanine amidase
41	UTI89_C3219 (argA)	3139678..3141009 [+], 1332	162138381	N-acetylglutamate synthase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

EC042_4529 , AHW84487

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	UTI89_C3196	3108499..3108990 [+], 492	91212190	secreted protein Hcp	EC042_4529
2	UTI89_C3199	3114339..3116108 [+], 1770	91212193	hypothetical protein	AHW84487

Download FASTA format files

Proteins        Genes

Homology Immunity protein list

AHW84488

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	UTI89_C3200	3116083..3116916 [+], 834	91212194	hypothetical protein	AHW84488

Download FASTA format files

Proteins        Genes

(1) Boyer F et al. (2009). Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 10:104. [PudMed:19284603]

This literature contains bioinformatics investigation