SecReT6
I. Summary
SecReT6 ID127 experimental
StrainMyxococcus xanthus DK 1622
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNC_008095
Location5996770..6055445
CommentA deletion covering MXAN_4807-MXAN_4813 shows no growth defects or motility defects; however, the mutant is unable to form mature fruiting bodies (A. Konovalova, unpublished data).
Functioneffector translocation
T6SS TypeType i; subtype i1
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number511111111
ComponentTssGTssJTssKTssM
Number1111
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1MXAN_RS232955992055..5993263 [+], 1209499874046glycosyltransferase 
2MXAN_RS233005993383..5993763 [+], 3811844041282response regulator 
3MXAN_RS233055993842..5995125 [+], 1284499874048HlyC/CorC family transporter 
4MXAN_RS233105995148..5996368 [-], 1221521966873fibronectin type III domain-containing protein 
5MXAN_RS233205996770..6001965 [-], 5196499874050hypothetical protein 
6MXAN_RS233256001993..6003840 [-], 1848499874051OmpA family protein 
7MXAN_RS23330 (tssI)6004071..6006335 [+], 2265499874052type VI secretion system tip protein VgrG 
8MXAN_RS23335 (tssJ)6006481..6007098 [+], 618499874053type VI secretion system lipoprotein TssJ 
9MXAN_RS23340 (tssK)6007095..6008462 [+], 1368499874054type VI secretion system baseplate subunit TssK 
10MXAN_RS233456008478..6009194 [+], 717499874055DotU family type IV/VI secretion system protein 
11MXAN_RS23350 (tssM)6009191..6012886 [+], 3696499874056type VI secretion system membrane subunit TssM 
12MXAN_RS23355 (tagF)6012888..6013820 [+], 933499874057type VI secretion system-associated protein TagF 
13MXAN_RS23360 (tssA)6013836..6015860 [+], 2025499874058type VI secretion system protein TssA 
14MXAN_RS23365 (tssB)6015943..6016437 [+], 495499874059type VI secretion system contractile sheath small subunit 
15MXAN_RS23370 (tssC)6016463..6017947 [+], 1485499874060type VI secretion system contractile sheath large subunit 
16MXAN_RS23375 (hcp)6018067..6018558 [+], 492499874061type VI secretion system tube protein Hcp 
17MXAN_RS23380 (tssE)6018642..6019037 [+], 396499874062type VI secretion system baseplate subunit TssE 
18MXAN_RS23385 (tssF)6019075..6020826 [+], 1752499874063type VI secretion system baseplate subunit TssF 
19MXAN_RS23390 (tssG)6020790..6021833 [+], 1044521966872type VI secretion system baseplate subunit TssG 
20MXAN_RS23395 (tssH)6021917..6024574 [+], 2658499874065type VI secretion system ATPase TssH 
21MXAN_RS234006024583..6025677 [+], 1095499874066hypothetical protein 
22MXAN_RS234056025827..6026192 [+], 366499874067HPF/RaiA family ribosome-associated protein 
23MXAN_RS234106026252..6026809 [+], 558499874068redoxin domain-containing protein 
24MXAN_RS234156026828..6028240 [-], 1413521966871glycoside hydrolase family 16 protein 
25MXAN_RS234206028288..6029355 [-], 1068499874070sulfate/molybdate ABC transporter ATP-binding protein 
26MXAN_RS23425 (cysW)6029352..6030200 [-], 849521966870sulfate ABC transporter permease subunit CysW 
27MXAN_RS23430 (cysT)6030206..6031048 [-], 843499874072sulfate ABC transporter permease subunit CysT 
28MXAN_RS234356031061..6032071 [-], 1011499874073sulfate ABC transporter substrate-binding protein 
29MXAN_RS234406032464..6035913 [+], 3450499874075AAA family ATPase 
30MXAN_RS234456035918..6039301 [+], 3384499874076ATP-dependent Clp protease ATP-binding subunit 
31MXAN_RS234506039303..6041597 [+], 2295521966868ATP-dependent Clp protease ATP-binding subunit 
32MXAN_RS234556041643..6042224 [-], 582499874078superoxide dismutase 
33MXAN_RS234606042250..6042750 [-], 501499874079flavin reductase 
34MXAN_RS234656043111..6045372 [-], 2262499874081xanthine dehydrogenase family protein molybdopterin-binding subunit 
35MXAN_RS234706045383..6045844 [-], 462499874082(2Fe-2S)-binding protein 
36MXAN_RS234756045841..6046494 [-], 654648422418hypothetical protein 
37MXAN_RS234806046572..6048992 [-], 24211976430757ATP-dependent Clp protease ATP-binding subunit 
38MXAN_RS234856049391..6049825 [+], 435499874085hypothetical protein 
39MXAN_RS234906049900..6050466 [+], 567499874087hypothetical protein 
40MXAN_RS234956050649..6052112 [+], 1464499874088MFS transporter 
41MXAN_RS235006052139..6053617 [-], 1479499874089glycoside hydrolase family 6 protein 
42MXAN_RS235056053772..6055445 [-], 1674499874090amidase 
43MXAN_RS235106055602..6057587 [+], 1986499874091ATP-dependent DNA helicase RecQ 
44MXAN_RS235156057594..6058550 [-], 957521966866pirin family protein 
45MXAN_RS235206058815..6060029 [-], 1215499874093protein kinase 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
MXAN_5572 , MXAN_4809, MXAN_0050

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

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Proteins        Genes

Homology Immunity protein list
MXAN_5571, MXAN_0049

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
2MXAN_RS0026056489..57073 [-], 585499869464hypothetical protein MXAN_0049
Data from experimental literature

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Proteins        Genes
(1) Chang YW et al. (2014). Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography. Nat Methods. 11(7):737-9. [PudMed:24813625] experimental
(2) Konovalova A et al. (2010). Extracellular biology of Myxococcus xanthus. FEMS Microbiol Rev. 34(2):89-106. [PudMed:19895646]
(3) Boyer F et al. (2009). Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 10:104. [PudMed:19284603] in_silico
 
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation