SecReT6
I. Summary
SecReT6 ID150 experimental
StrainPseudomonas aeruginosa UCBPP-PA14
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNC_008463
Location82730..121410
Commentimportant for resistance of biofilms to a subset of antibiotics; mediating P. aeruginosa pathogenesis
Functionanti-eukaryotic and anti-bacterial; effector translocation;virulence
T6SS TypeType i; subtype i3
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number111211111
ComponentTssGTssJTssKTssM
Number1111
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1PA14_RS0034578396..78671 [+], 276489174087YheV family putative metal-binding protein 
2PA14_RS0035078760..79818 [+], 1059489174091PA0069 family radical SAM protein 
3PA14_RS0035580049..80963 [-], 915489174093type VI secretion system-associated lipoprotein TagQ  TagQ
4PA14_RS0036081025..82737 [-], 1713489174095type VI secretion system-associated regulator TagR  TagR
5PA14_RS0036582730..83929 [-], 1200489228598type IV secretion associated ABC transporter permease TagS  TagS
6PA14_RS0037083929..84648 [-], 720489228599type IV secretion associated ABC transporter ATP-binding protein TagT  TagT
7PA14_RS00375 (ppkA)84645..87743 [-], 3099489228601serine/threonine protein kinase PpkA  PpkA
8PA14_RS0038087751..88479 [-], 729489174102serine/threonine phosphatase PppA  PppA
9PA14_RS00385 (tagF)88489..89169 [-], 681489174105type VI secretion system-associated protein TagF  TagF
10PA14_RS00390 (tssM)89166..92699 [-], 3534489202722type VI secretion system membrane subunit TssM 
11PA14_RS0039592696..94045 [-], 1350489206020DotU family type VI secretion system protein  TssL
12PA14_RS00400 (tssK)94052..95386 [-], 1335489206021type VI secretion system baseplate subunit TssK  TssK
13PA14_RS00405 (tssJ)95402..95866 [-], 465489174123type VI secretion system lipoprotein TssJ  TssJ
14PA14_RS00410 (tagH)95911..97409 [-], 1499pseudogenetype VI secretion system-associated FHA domain protein TagH 
15PA14_RS00415 (tssA)97777..98811 [+], 1035489202488type VI secretion system protein TssA  TssA
16PA14_RS00420 (tssB)98900..99418 [+], 519489174130type VI secretion system contractile sheath small subunit  TssB
17PA14_RS00425 (tssC)99431..100927 [+], 1497489202487type VI secretion system contractile sheath large subunit  TssC
18PA14_RS00430101003..101491 [+], 489489174134type VI secretion system receptor/chaperone Hcp  TssD
19PA14_RS00435101659..102504 [+], 846489210664type VI secretion system accessory protein TagJ  TagJ
20PA14_RS00440 (tssE)102506..103015 [+], 510489202486type VI secretion system baseplate subunit TssE  TssE
21PA14_RS00445 (tssF)103012..104871 [+], 1860489174140type VI secretion system baseplate subunit TssF  TssF
22PA14_RS00450 (tssG)104835..105881 [+], 1047489195656type VI secretion system baseplate subunit TssG  TssG
23PA14_RS00455 (tssH)105874..108582 [+], 2709489228610type VI secretion system ATPase TssH  TssH
24PA14_RS00460 (vgrG1a)108629..110560 [+], 1932499985809type VI secretion system spike protein VgrG1a  TssI
25PA14_RS00465110675..110959 [-], 285489202663hypothetical protein  Tsi6 (i)
26PA14_RS00470 (tis1)110972..111205 [-], 234489174153T6SS immunity factor Tis1 
27PA14_RS00475 (tas1)111195..112577 [-], 1383489174155T6SS effector (p)ppApp synthetase Tas1 
28PA14_RS00480112593..113027 [-], 435489174156DcrB-related protein 
29PA14_RS00485 (vgrG1b)113281..115506 [+], 2226499985811type VI secretion system spike protein VgrG1b  TssI
30PA14_RS00490115534..115983 [+], 450489228628hypothetical protein 
31PA14_RS00495115913..117112 [+], 1200489228629DUF2169 domain-containing protein 
32PA14_RS00500117109..118146 [+], 10384892286313-oxoacyl-ACP synthase 
33PA14_RS00505118146..119237 [+], 1092489228633DUF4150 domain-containing protein 
34PA14_RS00510119248..120168 [+], 921489228635hypothetical protein 
35PA14_RS00515120178..121410 [+], 1233489228637TIGR02270 family protein 
36PA14_RS00520121786..122514 [+], 729489174165carbonic anhydrase 
37PA14_RS00525122725..124296 [+], 1572489228640SulP family inorganic anion transporter 
38PA14_RS00530124283..124921 [-], 639510934210hypothetical protein 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
PA1844 , PA2684, PA2685, PA2702, PA2774, PA3484, PA0085, PA0091, PA0093, PA0095

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

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Proteins        Genes

Homology Immunity protein list
PA1845, PA2703, PA2775, PA3485, PA0092, PA14_01130

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
6PA14_RS00470(tis1)110972..111205 [-], 234489174153T6SS immunity factor Tis1 PA14_01130
Data from experimental literature

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Proteins        Genes
Regulatory element identified
WspR
RegulatorDescriptionReference
WspR (PA14_16500)ActivatorPMID: 21955777

LadS
RegulatorDescriptionReference
LadSActivatorPMID: 22216178

RetS
RegulatorDescriptionReference
RetS (PA14_64230)RepressorPMID: 22216178

RsmF
RegulatorDescriptionReference
RsmF (PA14_68470)RepressorPMID: 23980177

PA14_36990
RegulatorDescriptionReference
PA14_36990RepressorPMID: 21955777

The information on regulator interaction.

#homolog effector locus tagRegulator wayFunctionRegulate targetDescription
1PA14_22470 (PA3225)Transcription regulator+PA14_00990 (TssA) promotorPA3225 directly interacts with the tssABC1 promoter,and planktonic expression of tssA1 is decreased 2-fold in the absence of PA3225.
2PA14_52570 (RsmA)Post-transcriptional-TssA1 (PA14_00990) transcriptRsmA binds the tssA1 mRNA in a region overlapping the ribosome binding site (RBS), which is thought to form a stem-loop structure.
3PA14_52570 (RsmA)-TssA2 (PA14_43050) transcriptRsmA binds the tssA2 mRNA in a region overlapping the ribosome binding site (RBS), and contains the core GGA motif.
4PA14_52570 (RsmA)-TssB3 (PA14_34070) transcriptRsmA binds the tssB3 mRNA in a region overlapping the ribosome binding site (RBS), and contains the core GGA motif.
5PA14_20290 (AmrZ)Transcriptional+TssA1 (PA14_00990) promotorAmrZ binds to all three assessed T6SS promoter regions and activates expression of H1-T6SS (tssA1).
6PA14_20290 (AmrZ)Transcriptional-TssA2 (PA14_43050) promotorAmrZ binds to all three assessed T6SS promoter regions and represses H2-T6SS (tssB2) transcription and H2-T6SS bacterial Killing.
7PA14_20290 (AmrZ)Transcriptional+TssB3 (PA14_34070) promotorAmrZ binds to all three assessed T6SS promoter regions and activates expression of H3-T6SS (vgrG3).
(1) Hachani A et al. (2014). The VgrG proteins are "A la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 289(25):17872-17884. [PudMed:24794869] experimental
(2) Jones C et al. (2013). An rhs-encoding gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14. J Bacteriol. 196(4):800-10. [PudMed:24317402] experimental
(3) Mikkelsen H et al. (2011). The Pseudomonas aeruginosa reference strain PA14 displays increased virulence due to a mutation in ladS. PLoS One. 6(12):e29113. [PudMed:22216178] experimental
(4) Zhang L et al. (2011). Pseudomonas aeruginosa tssC1 links type VI secretion and biofilm-specific antibiotic resistance. J Bacteriol. 193(19):5510-3. [PudMed:21784934] experimental
(5) Barret M et al. (2011). Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537] in_silico
(6) Lesic B et al. (2009). Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology. 155(Pt 9):2845-55. [PudMed:19497948] experimental
 
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation