I. Summary
SecReT6 ID150 experimental
StrainPseudomonas aeruginosa UCBPP-PA14
Repliconchromosome [Browse all T6SS(s) in this replicon]
Commentimportant for resistance of biofilms to a subset of antibiotics; mediating P. aeruginosa pathogenesis
Functionanti-eukaryotic and anti-bacterial; effector translocation;virulence
T6SS TypeType i; subtype i3
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1PA14_0080078396..78671 [+], 276116053789hypothetical protein 
2PA14_0081078760..79818 [+], 1059116053790DNA repair photolyase 
3PA14_0082080049..80963 [-], 915116053791hypothetical protein  TagQ
4PA14_0083081025..82737 [-], 1713116053792hypothetical protein  TagR
5PA14_0085082730..83929 [-], 1200116053793hypothetical protein  TagS
6PA14_0086083929..84648 [-], 720116053794ABC transporter ATP-binding protein  TagT
7PA14_00875 (ppkA)84645..87743 [-], 3099116054435serine/threonine protein kinase PpkA  PpkA
8PA14_0089087751..88479 [-], 729116053795phosphoprotein phosphatase  PppA
9PA14_0090088489..89169 [-], 681116053796hypothetical protein  TagF
10PA14_0091089166..92474 [-], 3309116053797hypothetical protein  TssM
11PA14_0092592696..94045 [-], 1350116054405hypothetical protein  TssL
12PA14_0094094052..95386 [-], 1335116053798hypothetical protein  TssK
13PA14_0096095402..95866 [-], 465116053799lipoprotein  TssJ
14PA14_0097095911..96453 [-], 543116053800hypothetical protein  Fha
15PA14_0098096450..97409 [-], 960116053801hypothetical protein  Fha
16PA14_0099097777..98811 [+], 1035116053802hypothetical protein  TssA
17PA14_0101098900..99418 [+], 519116053803hypothetical protein  TssB
18PA14_0102099431..100927 [+], 1497116053804hypothetical protein  TssC
19PA14_01030101003..101491 [+], 489116053805hypothetical protein  TssD
20PA14_01040101659..102504 [+], 846116053806secretion protein  TagJ
21PA14_01060102506..103015 [+], 510116053807hypothetical protein  TssE
22PA14_01070103012..104871 [+], 1860116053808hypothetical protein  TssF
23PA14_01080104835..105881 [+], 1047116053809hypothetical protein  TssG
24PA14_01100105874..108582 [+], 2709116053810ClpA/B-type chaperone  TssH
25PA14_01110108629..110560 [+], 1932116053811hypothetical protein  TssI
26PA14_01120110675..110959 [-], 285116053812hypothetical protein  Tsi6 (i)
27PA14_01130110972..111205 [-], 234116053813hypothetical protein 
28PA14_01140111195..112514 [-], 1320116053814hypothetical protein  Tse6 (e)
29PA14_01150112593..113027 [-], 435116053815hypothetical protein 
30PA14_01160113281..115506 [+], 2226116053816hypothetical protein  TssI
31PA14_01170115534..115983 [+], 450116053817hypothetical protein 
32PA14_01180115913..117112 [+], 1200116053818hypothetical protein 
33PA14_01190117109..118146 [+], 10381160538193-oxoacyl-ACP synthase 
34PA14_01200118146..119237 [+], 1092116053820hypothetical protein 
35PA14_01220119248..120168 [+], 921116053821hypothetical protein 
36PA14_01230120178..121410 [+], 1233116053822hypothetical protein 
37PA14_01240121786..122514 [+], 729116053823carbonic anhydrase 
38PA14_01250122725..124296 [+], 1572116053824sulfate transporter 
39PA14_01270124433..125029 [+], 597116053825hypothetical protein 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
PA1844 , PA2684, PA2685, PA2702, PA2774, PA3484, PA0085, PA0091, PA0093, PA0095

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA14_406603630345..3630809 [+], 465116049796hypothetical protein PA1844
2PA14_294002546202..2550155 [+], 3954116050670hypothetical protein PA2684
3PA14_293902544083..2546152 [+], 2070116050671hypothetical protein PA2685
4PA14_292002524592..2525068 [-], 477116050687hypothetical protein PA2702
5PA14_282102441454..2442041 [+], 588116050774hypothetical protein PA2774
6PA14_190201642132..1643358 [-], 1227116051502hypothetical protein PA3484
7PA14_01030101003..101491 [+], 489116053805hypothetical protein PA0085
8PA14_01110108629..110560 [+], 1932116053811hypothetical protein PA0091
9PA14_01140111195..112514 [-], 1320116053814hypothetical protein PA0093
10PA14_01160113281..115506 [+], 2226116053816hypothetical protein PA0095
Data from experimental literature

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Proteins        Genes

Homology Immunity protein list
PA1845, PA2703, PA2775, PA3485, PA0092, PA14_01130

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA14_406503629785..3630303 [+], 519116049797hypothetical protein PA1845
2PA14_291902524349..2524582 [-], 234116050688hypothetical protein PA2703
3PA14_282002441012..2441449 [+], 438116050775hypothetical protein PA2775
4PA14_190101641698..1642135 [-], 438116051503hypothetical protein PA3485
5PA14_01120110675..110959 [-], 285116053812hypothetical protein PA0092

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Proteins        Genes
Regulatory element identified
WspR (PA14_16500)ActivatorPMID: 21955777

LadSActivatorPMID: 22216178

RetS (PA14_64230)RepressorPMID: 22216178

RsmF (PA14_68470)RepressorPMID: 23980177

PA14_36990RepressorPMID: 21955777

The information on regulator interaction.

#homolog effector locus tagRegulator wayFunctionRegulate targetDescription
1PA14_22470 (PA3225)Transcription regulator+PA14_00990 (TssA) promotorPA3225 directly interacts with the tssABC1 promoter,and planktonic expression of tssA1 is decreased 2-fold in the absence of PA3225.
2PA14_52570 (RsmA)Post-transcriptional-TssA1 (PA14_00990) transcriptRsmA binds the tssA1 mRNA in a region overlapping the ribosome binding site (RBS), which is thought to form a stem-loop structure.
3PA14_52570 (RsmA)-TssA2 (PA14_43050) transcriptRsmA binds the tssA2 mRNA in a region overlapping the ribosome binding site (RBS), and contains the core GGA motif.
4PA14_52570 (RsmA)-TssB3 (PA14_34070) transcriptRsmA binds the tssB3 mRNA in a region overlapping the ribosome binding site (RBS), and contains the core GGA motif.
5PA14_20290 (AmrZ)Transcriptional+TssA1 (PA14_00990) promotorAmrZ binds to all three assessed T6SS promoter regions and activates expression of H1-T6SS (tssA1).
6PA14_20290 (AmrZ)Transcriptional-TssA2 (PA14_43050) promotorAmrZ binds to all three assessed T6SS promoter regions and represses H2-T6SS (tssB2) transcription and H2-T6SS bacterial Killing.
7PA14_20290 (AmrZ)Transcriptional+TssB3 (PA14_34070) promotorAmrZ binds to all three assessed T6SS promoter regions and activates expression of H3-T6SS (vgrG3).
(1) Hachani A et al. (2014). The VgrG proteins are "A la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 289(25):17872-17884. [PudMed:24794869] experimental
(2) Jones C et al. (2013). An rhs-encoding gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14. J Bacteriol. 196(4):800-10. [PudMed:24317402] experimental
(3) Mikkelsen H et al. (2011). The Pseudomonas aeruginosa reference strain PA14 displays increased virulence due to a mutation in ladS. PLoS One. 6(12):e29113. [PudMed:22216178] experimental
(4) Zhang L et al. (2011). Pseudomonas aeruginosa tssC1 links type VI secretion and biofilm-specific antibiotic resistance. J Bacteriol. 193(19):5510-3. [PudMed:21784934] experimental
(5) Barret M et al. (2011). Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537] in_silico
(6) Lesic B et al. (2009). Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology. 155(Pt 9):2845-55. [PudMed:19497948] experimental
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation