I. Summary
SecReT6 ID152 experimental
StrainPseudomonas aeruginosa UCBPP-PA14
Repliconchromosome [Browse all T6SS(s) in this replicon]
CommentNeeded for full virulence in mouse lung and acute burn model; required for wild-type infection of Arabidopsis; A Rhs element and the cognate immunity protein are involved in the bacterial killing.
Functionanti-eukaryotic and anti-bacterial; virulence
T6SS TypeType i; subtype i1
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1PA14_42760 (aroC)3802844..3803935 [-], 1092116049630chorismate synthase 
2PA14_427703804043..3805026 [+], 984116049629hypothetical protein 
3PA14_427803805104..3805883 [+], 780116049628hypothetical protein 
4PA14_427903805908..3806822 [-], 915116049627N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase 
5PA14_428203806993..3807589 [+], 597116049626isochorismatase family hydrolase 
6PA14_428303807762..3808082 [+], 321116049625hypothetical protein 
7PA14_428403808139..3808696 [+], 558116049624hypothetical protein 
8PA14_42850 (folE)3808774..3809319 [+], 546116049623GTP cyclohydrolase I 
9PA14_428603809384..3809845 [-], 462116049622hypothetical protein 
10PA14_428703810092..3810472 [+], 381116049621hypothetical protein 
11PA14_42880 (stk1)3810500..3811489 [-], 990116049620serine-threonine kinase Stk1  Stk1
12PA14_42890 (stp1)3811486..3812214 [-], 729116049619serine/threonine phosphoprotein phosphatase Stp1  Stp1
13PA14_429003812214..3815741 [-], 3528116049618hypothetical protein  TssM
14PA14_429103815757..3816626 [-], 870116049617hypothetical protein  TssL
15PA14_429203816629..3817960 [-], 1332116049616hypothetical protein  TssK
16PA14_429403817957..3818463 [-], 507116049615lipoprotein  TssJ
17PA14_429503818469..3819662 [-], 1194116049614hypothetical protein  Fha
18PA14_429603819680..3819820 [-], 141116049613hypothetical protein 
19PA14_429703819909..3821420 [-], 1512116049612transcriptional regulator  Sfa2
20PA14_429803821431..3824064 [-], 2634116049611ClpA/B-type protease  TssH
21PA14_429903824071..3825078 [-], 1008116049610hypothetical protein  TssG
22PA14_430003825042..3826622 [-], 1581116049609hypothetical protein  TssF
23PA14_430203827167..3827574 [-], 408116049608hypothetical protein  TssE
24PA14_430303827587..3829062 [-], 1476116049607hypothetical protein  TssC
25PA14_430403829090..3829596 [-], 507116049606hypothetical protein  TssB
26PA14_430503829632..3831188 [-], 1557116049605hypothetical protein  TssA
27PA14_43070 (hcpD)3831886..3832404 [+], 519116049604secreted protein Hcp  TssD
28PA14_430803832600..3834642 [+], 2043116049603hypothetical protein  TssI
29PA14_430903834632..3835372 [+], 741116049602hypothetical protein 
30PA14_431003835369..3840213 [+], 4845116049601Rhs family protein  RhsP2 (e)
31PA14_431103841020..3841622 [-], 603116049600glutathione S-transferase 
32PA14_431303841678..3842844 [-], 1167116049599aminotransferase 
33PA14_431403842926..3843402 [+], 477116049598MarR family transcriptional regulator 
34PA14_431503843452..3844087 [-], 636116049597hypothetical protein 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
PSPTO_5435 , PA14_43100, PSPTO_5435, PA3487, PSPTO_5435, PSPTO_5435

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA14_44890(hcpA)4000090..4000608 [+], 519116049456secreted protein Hcp PSPTO_5435
2PA14_431003835369..3840213 [+], 4845116049601Rhs family protein PA14_43100
3PA14_43070(hcpD)3831886..3832404 [+], 519116049604secreted protein Hcp PSPTO_5435
4PA14_18970(pldA)1635662..1638973 [-], 3312116051504phospholipase D PA3487
5PA14_69560(hcpB)6206092..6206610 [-], 519116053416secreted protein Hcp PSPTO_5435
6PA14_03240(hcpC)288454..288972 [-], 519116053976secreted protein Hcp PSPTO_5435

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Proteins        Genes

Homology Immunity protein list

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

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Proteins        Genes
Regulatory element identified
LasR (PA14_45960)ActivatorPMID: 19497948

MvfR (PA14_51340)ActivatorPMID: 15686549

(1) Hachani A et al. (2014). The VgrG proteins are "A la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 289(25):17872-17884. [PudMed:24794869] experimental
(2) Jones C et al. (2013). An rhs-encoding gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14. J Bacteriol. 196(4):800-10. [PudMed:24317402] experimental
(3) Barret M et al. (2011). Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537] in_silico
(4) Lesic B et al. (2009). Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology. 155(Pt 9):2845-55. [PudMed:19497948] experimental
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation