SecReT6
I. Summary
SecReT6 ID152 experimental
StrainPseudomonas aeruginosa UCBPP-PA14
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNC_008463
Location3806993..3840213
CommentNeeded for full virulence in mouse lung and acute burn model; required for wild-type infection of Arabidopsis; A Rhs element and the cognate immunity protein are involved in the bacterial killing.
Functionanti-eukaryotic and anti-bacterial; virulence
T6SS TypeType i; subtype i1
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number111111111
ComponentTssGTssJTssKTssM
Number1111
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1PA14_RS17365 (aroC)3802844..3803935 [-], 1092489178143chorismate synthase 
2PA14_RS173703804043..3805026 [+], 984489231747alpha/beta hydrolase 
3PA14_RS173753805104..3805883 [+], 780489178139hypothetical protein 
4PA14_RS17380 (prmB)3805908..3806822 [-], 91548917813850S ribosomal protein L3 N(5)-glutamine methyltransferase 
5PA14_RS173853806993..3807589 [+], 597489231748cysteine hydrolase 
6PA14_RS173903807762..3808082 [+], 321489231749hypothetical protein 
7PA14_RS173953808139..3808696 [+], 558489231750Smr/MutS family protein 
8PA14_RS17400 (folE)3808774..3809319 [+], 546489178129GTP cyclohydrolase I FolE 
9PA14_RS174053809384..3809845 [-], 462489178127bacteriohemerythrin 
10PA14_RS174103810092..3810472 [+], 381489201244VOC family protein 
11PA14_RS174153810500..3811489 [-], 990489231751serine/threonine protein kinase  Stk1
12PA14_RS174203811486..3812214 [-], 729489201246serine/threonine-protein phosphatase  Stp1
13PA14_RS17425 (tssM)3812214..3815741 [-], 3528489231752type VI secretion system membrane subunit TssM  TssM
14PA14_RS174303815757..3816626 [-], 870489211559type IVB secretion system protein IcmH/DotU  TssL
15PA14_RS17435 (tssK)3816629..3817960 [-], 1332489178110type VI secretion system baseplate subunit TssK  TssK
16PA14_RS17440 (tssJ)3817957..3818463 [-], 507489201247type VI secretion system lipoprotein TssJ  TssJ
17PA14_RS17445 (tagH)3818469..3819662 [-], 1194489231753type VI secretion system-associated FHA domain protein TagH  Fha
18PA14_RS174503819680..3819820 [-], 141489178104hypothetical protein 
19PA14_RS174553819909..3821420 [-], 1512489231755sigma-54-dependent Fis family transcriptional regulator  Sfa2
20PA14_RS17460 (tssH)3821431..3824064 [-], 2634489231757type VI secretion system ATPase TssH  TssH
21PA14_RS17465 (tssG)3824071..3825078 [-], 1008489231760type VI secretion system baseplate subunit TssG  TssG
22PA14_RS17470 (tssF)3825042..3826829 [-], 1788489178095type VI secretion system baseplate subunit TssF 
23PA14_RS311553826989..3827156 [-], 168pseudogenePAAR domain-containing protein 
24PA14_RS17475 (tssE)3827167..3827574 [-], 408489231763type VI secretion system baseplate subunit TssE  TssE
25PA14_RS17480 (tssC)3827587..3829062 [-], 1476489178086type VI secretion system contractile sheath large subunit  TssC
26PA14_RS17485 (tssB)3829090..3829596 [-], 507489178082type VI secretion system contractile sheath small subunit  TssB
27PA14_RS17490 (tssA)3829632..3831188 [-], 1557489231764type VI secretion system protein TssA  TssA
28PA14_RS174953831886..3832404 [+], 519489173777Hcp family type VI secretion system effector  TssD
29PA14_RS175003832600..3834642 [+], 2043499985991type VI secretion system tip protein VgrG  TssI
30PA14_RS175053834632..3835372 [+], 741489231779DUF4123 domain-containing protein 
31PA14_RS175103835369..3840213 [+], 4845489231781RHS repeat protein  RhsP2 (e)
32PA14_RS306503840223..3840657 [+], 435510933967hypothetical protein 
33PA14_RS175153841020..3841622 [-], 603489188423glutathione S-transferase family protein 
34PA14_RS175203841678..3842844 [-], 1167489231784PLP-dependent aminotransferase family protein 
35PA14_RS175253842926..3843402 [+], 477489188426MarR family transcriptional regulator 
36PA14_RS175303843452..3844087 [-], 636489178063hypothetical protein 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
PSPTO_5435 , PA14_43100, PSPTO_5435, PA3487, PSPTO_5435, PSPTO_5435

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

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Proteins        Genes

Homology Immunity protein list
PA3488

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

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Proteins        Genes
Regulatory element identified
LasR
RegulatorDescriptionReference
LasR (PA14_45960)ActivatorPMID: 19497948

MvfR
RegulatorDescriptionReference
MvfR (PA14_51340)ActivatorPMID: 15686549

The information on regulator interaction.

#homolog effector locus tagRegulator wayFunctionRegulate targetDescription
1PA14_52570 (RsmA)Post-transcriptional-TssA1 (PA14_00990) transcriptRsmA binds the tssA1 mRNA in a region overlapping the ribosome binding site (RBS), which is thought to form a stem-loop structure.
2PA14_52570 (RsmA)-TssA2 (PA14_43050) transcriptRsmA binds the tssA2 mRNA in a region overlapping the ribosome binding site (RBS), and contains the core GGA motif.
3PA14_52570 (RsmA)-TssB3 (PA14_34070) transcriptRsmA binds the tssB3 mRNA in a region overlapping the ribosome binding site (RBS), and contains the core GGA motif.
4PA14_20290 (AmrZ)Transcriptional+TssA1 (PA14_00990) promotorAmrZ binds to all three assessed T6SS promoter regions and activates expression of H1-T6SS (tssA1).
5PA14_20290 (AmrZ)Transcriptional-TssA2 (PA14_43050) promotorAmrZ binds to all three assessed T6SS promoter regions and represses H2-T6SS (tssB2) transcription and H2-T6SS bacterial Killing.
6PA14_20290 (AmrZ)Transcriptional+TssB3 (PA14_34070) promotorAmrZ binds to all three assessed T6SS promoter regions and activates expression of H3-T6SS (vgrG3).
7PA14_48150 (QslA)QS regulator-H2-T6SS (PA14_43050) promotorThe expression of H2-T6SS machinery genes was controlled at a transcriptional level by QslA.
(1) Hachani A et al. (2014). The VgrG proteins are "A la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 289(25):17872-17884. [PudMed:24794869] experimental
(2) Jones C et al. (2013). An rhs-encoding gene linked to the second type VI secretion cluster is a feature of the Pseudomonas aeruginosa strain PA14. J Bacteriol. 196(4):800-10. [PudMed:24317402] experimental
(3) Barret M et al. (2011). Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537] in_silico
(4) Lesic B et al. (2009). Quorum sensing differentially regulates Pseudomonas aeruginosa type VI secretion locus I and homologous loci II and III, which are required for pathogenesis. Microbiology. 155(Pt 9):2845-55. [PudMed:19497948] experimental
 
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation