I. Summary
SecReT6 ID157 insolico
StrainAeromonas hydrophila subsp. hydrophila ATCC 7966
Repliconchromosome [Browse all T6SS(s) in this replicon]
T6SS TypeType i; subtype i1
insolico Bioinformatics investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1AHA_18141980299..1980856 [-], 558117617944integral membrane protein 
2AHA_18151980807..1981493 [+], 687117619891DTW domain-containing protein 
3AHA_1816 (exsB)1981516..1982223 [+], 708117619436exsB protein 
4AHA_18171982243..1982956 [+], 714117620682radical activating enzyme 
5AHA_18181982958..1983188 [+], 231117620321hypothetical protein 
6AHA_18191983185..1983748 [+], 564117618490ATP:cob(I)alamin adenosyltransferase 
7AHA_18201983815..1984219 [-], 405117618168DNA-binding protein StpA 
8AHA_18211984799..1986379 [+], 1581117620019Na(+)/H(+) antiporter family protein 
9AHA_1822 (tdk)1986466..1987047 [+], 582117619087thymidine kinase 
10AHA_18231987116..1988453 [-], 1338117620901hypothetical protein 
11AHA_18241988606..1989067 [+], 462117621567hypothetical protein 
12AHA_18261990515..1991033 [+], 519117620640hemolysin co-regulated protein  TssD
13AHA_18271991311..1993542 [+], 2232117621074VgrG protein  TssI
14AHA_18281993544..1994863 [+], 1320117619721hypothetical protein 
15AHA_18291994873..1995862 [+], 990117620164hypothetical protein 
16AHA_18301995977..1996522 [+], 546117619201hypothetical protein 
17AHA_18311996643..1998031 [+], 1389117618069hypothetical protein 
18AHA_18321998704..1999207 [+], 504117618948hypothetical protein  TssB
19AHA_18331999252..2000724 [+], 1473117621313hypothetical protein  TssC
20AHA_18342000731..2001162 [+], 432117617929hypothetical protein  TssE
21AHA_18352001166..2002932 [+], 1767117619565hypothetical protein  TssF
22AHA_18362002896..2003894 [+], 999117620147hypothetical protein  TssG
23AHA_18372003951..2005201 [+], 1251117619691hypothetical protein  Fha
24AHA_18382005201..2005716 [+], 516117620252lipoprotein  TssJ
25AHA_18392005719..2007053 [+], 1335117618870hypothetical protein  TssK
26AHA_18402007145..2007855 [+], 711117619347hypothetical protein  TssL
27AHA_18412007876..2010518 [+], 2643117617832ClpA/ClpB family protein  TssH
28AHA_18422010521..2012059 [+], 1539117617496sigma-54 dependent transcriptional regulator 
29AHA_18432012059..2012664 [+], 606117620275hypothetical protein 
30AHA_18442012673..2014109 [+], 1437117620760ImpA-related domain-containing protein  TssA
31AHA_18452014151..2017636 [+], 3486117618798ImcF family protein  TssM
32AHA_18462017683..2019113 [+], 1431117619389ImpA domain-containing protein  TssA
33AHA_18472019371..2019658 [+], 288117619593PAAR motif-containing protein  PAAR
34AHA_18482019669..2021714 [+], 2046117620092Rhs element Vgr family protein  TssI
35AHA_18492021749..2024304 [+], 2556117620566hypothetical protein 
36AHA_18502024307..2024711 [+], 405117621024hypothetical protein 
37AHA_18542027356..2028219 [+], 864117620767bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase 
38AHA_1855 (cspD)2028327..2028545 [-], 219117618294cold shock domain-containing protein CspD 
39AHA_1856 (clpS)2028774..2029091 [+], 318117619917ATP-dependent Clp protease adaptor protein ClpS 
40AHA_1857 (clpA)2029151..2031403 [+], 2253117621540ATP-dependent Clp protease, ATP-binding subunit ClpA  TssH
41AHA_1858 (infA)2031473..2031712 [-], 240117621081translation initiation factor IF-1 
42AHA_18592031761..2032477 [-], 717117620630arginyl-tRNA-protein transferase 
43AHA_1860 (aat)2032474..2033181 [-], 708117618258leucyl/phenylalanyl-tRNA--protein transferase 
44AHA_18612033201..2033671 [+], 471117618220surface antigen 
45AHA_18622033782..2035029 [-], 1248117619488response regulator protein 
46AHA_1863 (trxB)2035087..2036268 [-], 1182117618917thioredoxin-disulfide reductase 
47AHA_1864 (lrp)2036934..2037425 [+], 492117620059leucine-responsive transcriptional regulator 
48AHA_18652037627..2040149 [+], 2523117619696FtsK/SpoIIIE family protein 
49AHA_1866 (lolA)2040227..2040835 [+], 609117620952outer membrane lipoprotein carrier protein LolA 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
ECA2866 , ABG57133, ABG57151, ECA2866, ABG57133

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1AHA_11181211755..1212273 [+], 519117617709HcpA-like protein ECA2866
2AHA_11191212752..1215535 [+], 2784117619461Rhs element Vgr protein ABG57133
3AHA_18482019669..2021714 [+], 2046117620092Rhs element Vgr family protein ABG57151
4AHA_18261990515..1991033 [+], 519117620640hemolysin co-regulated protein ECA2866
5AHA_18271991311..1993542 [+], 2232117621074VgrG protein ABG57133

Download FASTA format files
Proteins        Genes
Regulatory element identified
AhyIActivatorPMID: 19729404
AhyRActivatorPMID: 19729404

VasHActivatorPMID: 21378190

(1) Sha J et al. (2013). Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology. 159(Pt 6):1120-35. [PudMed:23519162] experimental
(2) Bernard CS et al. (2011). Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins. J Bacteriol. 193(9):2158-67. [PudMed:21378190] experimental
(3) Suarez G et al. (2010). Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology. 156(Pt 12):3678-88. [PudMed:20798163] experimental
(4) Suarez G et al. (2010). A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 192(1):155-68. [PudMed:19880608] experimental
(5) Khajanchi BK et al. (2009). N-acylhomoserine lactones involved in quorum sensing control the type VI secretion system, biofilm formation, protease production, and in vivo virulence in a clinical isolate of Aeromonas hydrophila. Microbiology. 155(Pt 11):3518-31. [PudMed:19729404] experimental
(6) Suarez G et al. (2008). Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 44(4):344-61. [PudMed:18037263] experimental
experimental This literature contains experimental investigation