 |
III. genome coordinates of the T6SS gene cluster |
# | Locus tag (Gene) | Coordinates [+/-], size (bp) | Protein GI | Product | Note |
1 | AHA_RS09135 (rrtA) | 1980299..1980856 [-], 558 | 500024977 | rhombosortase | |
2 | AHA_RS09140 | 1980807..1981493 [+], 687 | 500024978 | DTW domain-containing protein | |
3 | AHA_RS09145 (queC) | 1981516..1982223 [+], 708 | 500024979 | 7-cyano-7-deazaguanine synthase QueC | |
4 | AHA_RS09150 (queE) | 1982285..1982956 [+], 672 | 796480321 | 7-carboxy-7-deazaguanine synthase QueE | |
5 | AHA_RS09155 | 1982958..1983188 [+], 231 | 500024981 | DUF3820 family protein | |
6 | AHA_RS09160 | 1983185..1983748 [+], 564 | 500024982 | cob(I)yrinic acid a,c-diamide adenosyltransferase | |
7 | AHA_RS09165 | 1983815..1984219 [-], 405 | 500024983 | H-NS histone family protein | |
8 | AHA_RS09170 | 1984799..1986379 [+], 1581 | 500024984 | Na+/H+ antiporter NhaC family protein | |
9 | AHA_RS09175 | 1986466..1987047 [+], 582 | 500024985 | thymidine kinase | |
10 | AHA_RS09180 | 1987116..1988453 [-], 1338 | 500024986 | flavohemoglobin expression-modulating QEGLA motif protein | |
11 | AHA_RS09185 | 1988612..1989067 [+], 456 | 1818942453 | YbjN domain-containing protein | |
12 | AHA_RS09200 (hcp1) | 1990515..1991033 [+], 519 | 500024988 | type VI secretion system effector Hcp1 | |
13 | AHA_RS09205 (vgrG) | 1991311..1993542 [+], 2232 | 500024989 | type VI secretion system effector VgrG | |
14 | AHA_RS09210 | 1993544..1994863 [+], 1320 | 500024990 | type VI secretion system polymorphic nuclease effector | |
15 | AHA_RS09215 | 1994873..1995862 [+], 990 | 500024991 | type VI secretion system immunity protein | |
16 | AHA_RS09220 | 1995977..1996522 [+], 546 | 500024992 | hypothetical protein | |
17 | AHA_RS09225 | 1996658..1998031 [+], 1374 | 1818942454 | sel1 repeat family protein | |
18 | AHA_RS09230 (tssB) | 1998704..1999207 [+], 504 | 500024994 | type VI secretion system contractile sheath small subunit | TssB |
19 | AHA_RS09235 (tssC) | 1999246..2000724 [+], 1479 | 759440874 | type VI secretion system contractile sheath large subunit | |
20 | AHA_RS09240 (tssE) | 2000731..2001162 [+], 432 | 500024996 | type VI secretion system baseplate subunit TssE | TssE |
21 | AHA_RS09245 (tssF) | 2001166..2002932 [+], 1767 | 500024997 | type VI secretion system baseplate subunit TssF | |
22 | AHA_RS09250 (tssG) | 2002896..2003894 [+], 999 | 500024998 | type VI secretion system baseplate subunit TssG | |
23 | AHA_RS09255 (tagH) | 2003951..2005201 [+], 1251 | 500024999 | type VI secretion system-associated FHA domain protein TagH | |
24 | AHA_RS09260 (tssJ) | 2005201..2005716 [+], 516 | 500025000 | type VI secretion system lipoprotein TssJ | TssJ |
25 | AHA_RS09265 (tssK) | 2005719..2007053 [+], 1335 | 500025001 | type VI secretion system baseplate subunit TssK | |
26 | AHA_RS09270 (icmH) | 2007076..2007855 [+], 780 | 498320072 | type IVB secretion system protein IcmH/DotU | TssL |
27 | AHA_RS09275 (tssH) | 2007876..2010518 [+], 2643 | 500025003 | type VI secretion system ATPase TssH | |
28 | AHA_RS09280 (vasH) | 2010521..2012059 [+], 1539 | 500025004 | sigma-54-dependent transcriptional regulator VasH | |
29 | AHA_RS09285 (tagO) | 2012059..2012664 [+], 606 | 500025005 | type VI secretion system-associated protein TagO | |
30 | AHA_RS09290 (tssA) | 2012673..2014109 [+], 1437 | 500025006 | type VI secretion system protein TssA | |
31 | AHA_RS09295 (tssM) | 2014151..2017636 [+], 3486 | 500025007 | type VI secretion system membrane subunit TssM | |
32 | AHA_RS09300 | 2017683..2019113 [+], 1431 | 500025008 | type VI secretion system ImpA family N-terminal domain-containing protein | |
33 | AHA_RS09305 | 2019371..2019658 [+], 288 | 500025009 | type VI secretion system PAAR protein | |
34 | AHA_RS09310 | 2019669..2021714 [+], 2046 | 500025010 | type VI secretion system tip protein VgrG | |
35 | AHA_RS09315 | 2021749..2024304 [+], 2556 | 500025011 | hypothetical protein | |
36 | AHA_RS09320 | 2024307..2024711 [+], 405 | 500025012 | DUF1311 domain-containing protein | |
37 | AHA_RS09325 | 2025132..2025410 [+], 279 | 1690501459 | hypothetical protein | |
38 | AHA_RS09330 | 2025595..2025732 [-], 138 | pseudogene | IS5/IS1182 family transposase | |
39 | AHA_RS09335 | 2025781..2026269 [+], 489 | 1690501460 | hypothetical protein | |
40 | AHA_RS09355 (folD) | 2027356..2028219 [+], 864 | 500025013 | bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD | |
41 | AHA_RS09360 (cspD) | 2028327..2028545 [-], 219 | 500025014 | cold shock-like protein CspD | |
42 | AHA_RS09365 (clpS) | 2028774..2029091 [+], 318 | 491442239 | ATP-dependent Clp protease adapter ClpS | |
43 | AHA_RS09370 (clpA) | 2029151..2031403 [+], 2253 | 500025015 | ATP-dependent Clp protease ATP-binding subunit ClpA | |
44 | AHA_RS09375 (infA) | 2031473..2031691 [-], 219 | 491442244 | translation initiation factor IF-1 | |
45 | AHA_RS09380 | 2031761..2032477 [-], 717 | 500025017 | arginyltransferase | |
46 | AHA_RS09385 (aat) | 2032474..2033181 [-], 708 | 500025018 | leucyl/phenylalanyl-tRNA--protein transferase | |
47 | AHA_RS09390 | 2033201..2033671 [+], 471 | 500025019 | glycine zipper 2TM domain-containing protein | |
48 | AHA_RS09395 | 2033782..2035029 [-], 1248 | 500025020 | response regulator | |
49 | AHA_RS09400 (trxB) | 2035087..2036037 [-], 951 | 1818942455 | thioredoxin-disulfide reductase | |
50 | AHA_RS09405 | 2036554..2036784 [-], 231 | 1148649539 | alanine dehydrogenase | |
51 | AHA_RS09410 (lrp) | 2036934..2037425 [+], 492 | 500025022 | leucine-responsive transcriptional regulator Lrp | |
52 | AHA_RS09415 | 2037627..2040149 [+], 2523 | 500025023 | DNA translocase FtsK | |
53 | AHA_RS09420 (lolA) | 2040227..2040835 [+], 609 | 500025024 | outer membrane lipoprotein chaperone LolA | |
|
Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein