I. Summary

SecReT6 ID	157
Strain	Aeromonas hydrophila subsp. hydrophila ATCC 7966
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_008570
Location	1984799..2036268
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	2	1	1	2	2	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	AHA_1814	1980299..1980856 [-], 558	117617944	integral membrane protein
2	AHA_1815	1980807..1981493 [+], 687	117619891	DTW domain-containing protein
3	AHA_1816 (exsB)	1981516..1982223 [+], 708	117619436	exsB protein
4	AHA_1817	1982243..1982956 [+], 714	117620682	radical activating enzyme
5	AHA_1818	1982958..1983188 [+], 231	117620321	hypothetical protein
6	AHA_1819	1983185..1983748 [+], 564	117618490	ATP:cob(I)alamin adenosyltransferase
7	AHA_1820	1983815..1984219 [-], 405	117618168	DNA-binding protein StpA
8	AHA_1821	1984799..1986379 [+], 1581	117620019	Na(+)/H(+) antiporter family protein
9	AHA_1822 (tdk)	1986466..1987047 [+], 582	117619087	thymidine kinase
10	AHA_1823	1987116..1988453 [-], 1338	117620901	hypothetical protein
11	AHA_1824	1988606..1989067 [+], 462	117621567	hypothetical protein
12	AHA_1826	1990515..1991033 [+], 519	117620640	hemolysin co-regulated protein	TssD
13	AHA_1827	1991311..1993542 [+], 2232	117621074	VgrG protein	TssI
14	AHA_1828	1993544..1994863 [+], 1320	117619721	hypothetical protein
15	AHA_1829	1994873..1995862 [+], 990	117620164	hypothetical protein
16	AHA_1830	1995977..1996522 [+], 546	117619201	hypothetical protein
17	AHA_1831	1996643..1998031 [+], 1389	117618069	hypothetical protein
18	AHA_1832	1998704..1999207 [+], 504	117618948	hypothetical protein	TssB
19	AHA_1833	1999252..2000724 [+], 1473	117621313	hypothetical protein	TssC
20	AHA_1834	2000731..2001162 [+], 432	117617929	hypothetical protein	TssE
21	AHA_1835	2001166..2002932 [+], 1767	117619565	hypothetical protein	TssF
22	AHA_1836	2002896..2003894 [+], 999	117620147	hypothetical protein	TssG
23	AHA_1837	2003951..2005201 [+], 1251	117619691	hypothetical protein	Fha
24	AHA_1838	2005201..2005716 [+], 516	117620252	lipoprotein	TssJ
25	AHA_1839	2005719..2007053 [+], 1335	117618870	hypothetical protein	TssK
26	AHA_1840	2007145..2007855 [+], 711	117619347	hypothetical protein	TssL
27	AHA_1841	2007876..2010518 [+], 2643	117617832	ClpA/ClpB family protein	TssH
28	AHA_1842	2010521..2012059 [+], 1539	117617496	sigma-54 dependent transcriptional regulator
29	AHA_1843	2012059..2012664 [+], 606	117620275	hypothetical protein
30	AHA_1844	2012673..2014109 [+], 1437	117620760	ImpA-related domain-containing protein	TssA
31	AHA_1845	2014151..2017636 [+], 3486	117618798	ImcF family protein	TssM
32	AHA_1846	2017683..2019113 [+], 1431	117619389	ImpA domain-containing protein	TssA
33	AHA_1847	2019371..2019658 [+], 288	117619593	PAAR motif-containing protein	PAAR
34	AHA_1848	2019669..2021714 [+], 2046	117620092	Rhs element Vgr family protein	TssI
35	AHA_1849	2021749..2024304 [+], 2556	117620566	hypothetical protein
36	AHA_1850	2024307..2024711 [+], 405	117621024	hypothetical protein
37	AHA_1854	2027356..2028219 [+], 864	117620767	bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase
38	AHA_1855 (cspD)	2028327..2028545 [-], 219	117618294	cold shock domain-containing protein CspD
39	AHA_1856 (clpS)	2028774..2029091 [+], 318	117619917	ATP-dependent Clp protease adaptor protein ClpS
40	AHA_1857 (clpA)	2029151..2031403 [+], 2253	117621540	ATP-dependent Clp protease, ATP-binding subunit ClpA	TssH
41	AHA_1858 (infA)	2031473..2031712 [-], 240	117621081	translation initiation factor IF-1
42	AHA_1859	2031761..2032477 [-], 717	117620630	arginyl-tRNA-protein transferase
43	AHA_1860 (aat)	2032474..2033181 [-], 708	117618258	leucyl/phenylalanyl-tRNA--protein transferase
44	AHA_1861	2033201..2033671 [+], 471	117618220	surface antigen
45	AHA_1862	2033782..2035029 [-], 1248	117619488	response regulator protein
46	AHA_1863 (trxB)	2035087..2036268 [-], 1182	117618917	thioredoxin-disulfide reductase
47	AHA_1864 (lrp)	2036934..2037425 [+], 492	117620059	leucine-responsive transcriptional regulator
48	AHA_1865	2037627..2040149 [+], 2523	117619696	FtsK/SpoIIIE family protein
49	AHA_1866 (lolA)	2040227..2040835 [+], 609	117620952	outer membrane lipoprotein carrier protein LolA

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files

Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

ECA2866 , ABG57133, ABG57151, ECA2866, ABG57133

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	AHA_1118	1211755..1212273 [+], 519	117617709	HcpA-like protein	ECA2866
2	AHA_1119	1212752..1215535 [+], 2784	117619461	Rhs element Vgr protein	ABG57133
3	AHA_1848	2019669..2021714 [+], 2046	117620092	Rhs element Vgr family protein	ABG57151
4	AHA_1826	1990515..1991033 [+], 519	117620640	hemolysin co-regulated protein	ECA2866
5	AHA_1827	1991311..1993542 [+], 2232	117621074	VgrG protein	ABG57133

Download FASTA format files

Proteins        Genes

N/A

Regulatory element identified

AhyR

Regulator	Description	Reference
AhyI	Activator	PMID: 19729404
AhyR	Activator	PMID: 19729404

VasH

Regulator	Description	Reference
VasH	Activator	PMID: 21378190

(1) Sha J et al. (2013). Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology. 159(Pt 6):1120-35. [PudMed:23519162]

(2) Bernard CS et al. (2011). Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins. J Bacteriol. 193(9):2158-67. [PudMed:21378190]

(3) Suarez G et al. (2010). Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology. 156(Pt 12):3678-88. [PudMed:20798163]

(4) Suarez G et al. (2010). A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 192(1):155-68. [PudMed:19880608]

(5) Khajanchi BK et al. (2009). N-acylhomoserine lactones involved in quorum sensing control the type VI secretion system, biofilm formation, protease production, and in vivo virulence in a clinical isolate of Aeromonas hydrophila. Microbiology. 155(Pt 11):3518-31. [PudMed:19729404]

(6) Suarez G et al. (2008). Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 44(4):344-61. [PudMed:18037263]

This literature contains experimental investigation