SecReT6
I. Summary
SecReT6 ID157 insolico
StrainAeromonas hydrophila subsp. hydrophila ATCC 7966
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNC_008570
Location1984799..2036268
Comment
FunctionN/A
T6SS TypeType i; subtype i1
insolico Bioinformatics investigation has been performed on this T6SS.

II. T6SS components
ComponentTssHTssDTssLTssITssATssBTssCTssETssF
Number211221111
ComponentTssGTssJTssKTssM
Number1111
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1AHA_RS09135 (rrtA)1980299..1980856 [-], 558500024977rhombosortase 
2AHA_RS091401980807..1981493 [+], 687500024978DTW domain-containing protein 
3AHA_RS09145 (queC)1981516..1982223 [+], 7085000249797-cyano-7-deazaguanine synthase QueC 
4AHA_RS09150 (queE)1982285..1982956 [+], 6727964803217-carboxy-7-deazaguanine synthase QueE 
5AHA_RS091551982958..1983188 [+], 231500024981DUF3820 family protein 
6AHA_RS091601983185..1983748 [+], 564500024982cob(I)yrinic acid a,c-diamide adenosyltransferase 
7AHA_RS091651983815..1984219 [-], 405500024983H-NS histone family protein 
8AHA_RS091701984799..1986379 [+], 1581500024984Na+/H+ antiporter NhaC family protein 
9AHA_RS091751986466..1987047 [+], 582500024985thymidine kinase 
10AHA_RS091801987116..1988453 [-], 1338500024986flavohemoglobin expression-modulating QEGLA motif protein 
11AHA_RS091851988612..1989067 [+], 4561818942453YbjN domain-containing protein 
12AHA_RS09200 (hcp1)1990515..1991033 [+], 519500024988type VI secretion system effector Hcp1 
13AHA_RS09205 (vgrG)1991311..1993542 [+], 2232500024989type VI secretion system effector VgrG 
14AHA_RS092101993544..1994863 [+], 1320500024990type VI secretion system polymorphic nuclease effector 
15AHA_RS092151994873..1995862 [+], 990500024991type VI secretion system immunity protein 
16AHA_RS092201995977..1996522 [+], 546500024992hypothetical protein 
17AHA_RS092251996658..1998031 [+], 13741818942454sel1 repeat family protein 
18AHA_RS09230 (tssB)1998704..1999207 [+], 504500024994type VI secretion system contractile sheath small subunit  TssB
19AHA_RS09235 (tssC)1999246..2000724 [+], 1479759440874type VI secretion system contractile sheath large subunit 
20AHA_RS09240 (tssE)2000731..2001162 [+], 432500024996type VI secretion system baseplate subunit TssE  TssE
21AHA_RS09245 (tssF)2001166..2002932 [+], 1767500024997type VI secretion system baseplate subunit TssF 
22AHA_RS09250 (tssG)2002896..2003894 [+], 999500024998type VI secretion system baseplate subunit TssG 
23AHA_RS09255 (tagH)2003951..2005201 [+], 1251500024999type VI secretion system-associated FHA domain protein TagH 
24AHA_RS09260 (tssJ)2005201..2005716 [+], 516500025000type VI secretion system lipoprotein TssJ  TssJ
25AHA_RS09265 (tssK)2005719..2007053 [+], 1335500025001type VI secretion system baseplate subunit TssK 
26AHA_RS09270 (icmH)2007076..2007855 [+], 780498320072type IVB secretion system protein IcmH/DotU  TssL
27AHA_RS09275 (tssH)2007876..2010518 [+], 2643500025003type VI secretion system ATPase TssH 
28AHA_RS09280 (vasH)2010521..2012059 [+], 1539500025004sigma-54-dependent transcriptional regulator VasH 
29AHA_RS09285 (tagO)2012059..2012664 [+], 606500025005type VI secretion system-associated protein TagO 
30AHA_RS09290 (tssA)2012673..2014109 [+], 1437500025006type VI secretion system protein TssA 
31AHA_RS09295 (tssM)2014151..2017636 [+], 3486500025007type VI secretion system membrane subunit TssM 
32AHA_RS093002017683..2019113 [+], 1431500025008type VI secretion system ImpA family N-terminal domain-containing protein 
33AHA_RS093052019371..2019658 [+], 288500025009type VI secretion system PAAR protein 
34AHA_RS093102019669..2021714 [+], 2046500025010type VI secretion system tip protein VgrG 
35AHA_RS093152021749..2024304 [+], 2556500025011hypothetical protein 
36AHA_RS093202024307..2024711 [+], 405500025012DUF1311 domain-containing protein 
37AHA_RS093252025132..2025410 [+], 2791690501459hypothetical protein 
38AHA_RS093302025595..2025732 [-], 138pseudogeneIS5/IS1182 family transposase 
39AHA_RS093352025781..2026269 [+], 4891690501460hypothetical protein 
40AHA_RS09355 (folD)2027356..2028219 [+], 864500025013bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD 
41AHA_RS09360 (cspD)2028327..2028545 [-], 219500025014cold shock-like protein CspD 
42AHA_RS09365 (clpS)2028774..2029091 [+], 318491442239ATP-dependent Clp protease adapter ClpS 
43AHA_RS09370 (clpA)2029151..2031403 [+], 2253500025015ATP-dependent Clp protease ATP-binding subunit ClpA 
44AHA_RS09375 (infA)2031473..2031691 [-], 219491442244translation initiation factor IF-1 
45AHA_RS093802031761..2032477 [-], 717500025017arginyltransferase 
46AHA_RS09385 (aat)2032474..2033181 [-], 708500025018leucyl/phenylalanyl-tRNA--protein transferase 
47AHA_RS093902033201..2033671 [+], 471500025019glycine zipper 2TM domain-containing protein 
48AHA_RS093952033782..2035029 [-], 1248500025020response regulator 
49AHA_RS09400 (trxB)2035087..2036037 [-], 9511818942455thioredoxin-disulfide reductase 
50AHA_RS094052036554..2036784 [-], 2311148649539alanine dehydrogenase 
51AHA_RS09410 (lrp)2036934..2037425 [+], 492500025022leucine-responsive transcriptional regulator Lrp 
52AHA_RS094152037627..2040149 [+], 2523500025023DNA translocase FtsK 
53AHA_RS09420 (lolA)2040227..2040835 [+], 609500025024outer membrane lipoprotein chaperone LolA 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
ECA2866 , ABG57133, ABG57151, ECA2866, ABG57133

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

Download FASTA format files
Proteins        Genes
N/A
Regulatory element identified
AhyR
RegulatorDescriptionReference
AhyIActivatorPMID: 19729404
AhyRActivatorPMID: 19729404

VasH
RegulatorDescriptionReference
VasHActivatorPMID: 21378190

(1) Sha J et al. (2013). Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis. Microbiology. 159(Pt 6):1120-35. [PudMed:23519162] experimental
(2) Bernard CS et al. (2011). Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins. J Bacteriol. 193(9):2158-67. [PudMed:21378190] experimental
(3) Suarez G et al. (2010). Role of Hcp, a type 6 secretion system effector, of Aeromonas hydrophila in modulating activation of host immune cells. Microbiology. 156(Pt 12):3678-88. [PudMed:20798163] experimental
(4) Suarez G et al. (2010). A type VI secretion system effector protein, VgrG1, from Aeromonas hydrophila that induces host cell toxicity by ADP ribosylation of actin. J Bacteriol. 192(1):155-68. [PudMed:19880608] experimental
(5) Khajanchi BK et al. (2009). N-acylhomoserine lactones involved in quorum sensing control the type VI secretion system, biofilm formation, protease production, and in vivo virulence in a clinical isolate of Aeromonas hydrophila. Microbiology. 155(Pt 11):3518-31. [PudMed:19729404] experimental
(6) Suarez G et al. (2008). Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog. 44(4):344-61. [PudMed:18037263] experimental
 
experimental This literature contains experimental investigation