SecReT6
I. Summary
SecReT6 ID158 experimental
StrainFrancisella novicida U112
Repliconchromosome [Browse all T6SS(s) in this replicon]
AccessionNC_008601
Location1378294..1404786
CommentIntracellular growth in cultured murine macrophages
Functionanti-eukaryotic; effector translocation;virulence
T6SS TypeType ii (Type FPI)
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
ComponentPdpAPdpBIglEVgrGIglFIglGIglHDotUIglI
Number111111111
ComponentIglJPdpCPdpEIglDIglCIglBIglAPdpD
Number11111111
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1FTN_1309 (pdpA)1378294..1380756 [+], 2463118497889hypothetical protein  PdpA
2FTN_1310 (pdpB)1380761..1384042 [+], 3282118497890hypothetical protein  PdpB
3FTN_13111384032..1384409 [+], 378118497891hypothetical protein  IglE
4FTN_13121384422..1384916 [+], 495118497892hypothetical protein  VgrG
5FTN_13131384921..1386651 [+], 1731118497893hypothetical protein  IglF
6FTN_13141386652..1387173 [+], 522118497894hypothetical protein  IglG
7FTN_13151387186..1388616 [+], 1431118497895hypothetical protein  IglH
8FTN_13161388629..1389252 [+], 624118497896hypothetical protein  DotU
9FTN_13171389274..1390425 [+], 1152118497897hypothetical protein  IglI
10FTN_13181390391..1391164 [+], 774118497898hypothetical protein  IglJ
11FTN_1319 (pdpC)1391204..1395181 [+], 3978118497899hypothetical protein  PdpC
12FTN_13201395199..1395765 [+], 567118497900hypothetical protein  PdpE
13FTN_1321 (iglD)1395772..1396968 [-], 1197118497901intracellular growth locus protein D  IglD
14FTN_1322 (iglC)1396989..1397618 [-], 630118497902intracellular growth locus protein C  IglC
15FTN_1323 (iglB)1397635..1399155 [-], 1521118497903intracellular growth locus protein B  IglB
16FTN_1324 (iglA)1399169..1399723 [-], 555118497904intracellular growth locus protein A  IglA
17FTN_1325 (pdpD)1399803..1403540 [-], 3738118497905hypothetical protein  PdpD
18FTN_1326 (anmK)1403641..1404786 [-], 1146118497906anhydro-N-acetylmuramic acid kinase 
19FTN_1328 (treA)1406119..1407573 [+], 1455118497907trehalase 
20FTN_1329 (fbaA)1407740..1408804 [-], 1065118497908fructose-1,6-bisphosphate aldolase 
 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.
 






2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.
 






3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.
 







Homology Effector list
FTN_0131 , FTN_1069, FTN_1070, FTN_1071, FTN_1309, FTN_1312 (VgrG), FTN_1317 (IglI), FTN_1319, FTN_1322, FTN_1325

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1FTN_0131143587..144894 [-], 1308118496746hypothetical protein FTN_0131
2FTN_10691128155..1130653 [-], 2499118497660hypothetical protein FTN_1069
3FTN_10701130861..1132213 [-], 1353118497661hypothetical protein FTN_1070
4FTN_10711132421..1133899 [-], 1479118497662hypothetical protein FTN_1071
5FTN_1309(pdpA)1378294..1380756 [+], 2463118497889hypothetical protein FTN_1309
6FTN_13121384422..1384916 [+], 495118497892hypothetical protein (VgrG)FTN_1312 (VgrG)
7FTN_13171389274..1390425 [+], 1152118497897hypothetical protein (IglI)FTN_1317 (IglI)
8FTN_1319(pdpC)1391204..1395181 [+], 3978118497899hypothetical protein FTN_1319
9FTN_1322(iglC)1396989..1397618 [-], 630118497902intracellular growth locus protein C FTN_1322
10FTN_1325(pdpD)1399803..1403540 [-], 3738118497905hypothetical protein FTN_1325
Data from experimental literature

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Proteins        Genes
N/A
Regulatory element identified
KdpD
RegulatorDescriptionReference
KdpD (FTN_1715)Indirect activatorPMID: 20231408

FevR
RegulatorDescriptionReference
FevR (FTN_0480)Indirect activatorPMID: 18559431

MglA
RegulatorDescriptionReference
MglA (FTN_1290)ActivatorPMID: 20231408

SspA
RegulatorDescriptionReference
SspA (FTN_0549)ActivatorPMID: 20231408

KdpE
RegulatorDescriptionReference
KdpE (FTN_1714)Indirect repressorPMID: 20231408

FTN_1453
RegulatorDescriptionReference
FTN_1453RepressorPMID: 20231408

(1) Hare RF et al. (2014). Francisella novicida Pathogenicity Island Encoded Proteins Were Secreted during Infection of Macrophage-Like Cells. PLoS One. 9(8):e105773. [PudMed:25158041] experimental
(2) Broms JE et al. (2012). Unique Substrates Secreted by the Type VI Secretion System of Francisella tularensis during Intramacrophage Infection. PLoS One. 7(11):e50473. [PudMed:23185631] experimental
(3) de Bruin OM et al. (2011). The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology. 157(Pt 12):3483-91. [PudMed:21980115] experimental
(4) Robb CS et al. (2010). Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of intracellular growth locus E (IglE) protein from Francisella tularensis subsp. novicida. Acta Crystallogr Sect F Struct Biol Cryst Commun. 66(Pt 12):1596-8. [PudMed:21139203]
(5) Barker JR et al. (2009). The Francisella tularensis pathogenicity island encodes a secretion system that is required for phagosome escape and virulence. Mol Microbiol. 74(6):1459-70. [PudMed:20054881] experimental
(6) Nano FE et al. (2007). The Francisella pathogenicity island. Ann N Y Acad Sci. 1105:122-37. [PudMed:17395722]
 
experimental This literature contains experimental investigation