I. Summary
SecReT6 ID161 experimental
StrainAzoarcus sp. BH72
Repliconchromosome [Browse all T6SS(s) in this replicon]
CommentThe T6SS mutant appeared to be a better rice root colonizer as compared with the wild type.
Functioneffector translocation
T6SS TypeType i; subtype i3
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1AZO_RS194004238668..4239555 [-], 888500091575transporter 
2AZO_RS194054239989..4240999 [+], 1011500091576thiamine pyrophosphate-dependent dehydrogenase E1 component subunit alpha 
3AZO_RS194104241016..4242053 [+], 1038500091577alpha-ketoacid dehydrogenase subunit beta 
4AZO_RS194154242264..4242509 [+], 246500091578lipoyl domain-containing protein 
5AZO_RS194204242513..4243226 [+], 7145000915792-oxo acid dehydrogenase subunit E2 
6AZO_RS194254243226..4243996 [+], 771500091580lipoate--protein ligase 
7AZO_RS194304244089..4244643 [+], 555753917945twin-arginine translocation signal domain-containing protein 
8AZO_RS194354244733..4246307 [+], 1575500091582GMC family oxidoreductase 
9AZO_RS194404246369..4246983 [+], 615500091583VOC family protein 
10AZO_RS194454247215..4249182 [+], 1968500091584sigma-54-dependent Fis family transcriptional regulator 
11AZO_RS19450 (tssI)4249321..4251393 [+], 2073500091585type VI secretion system tip protein VgrG 
12AZO_RS194554251398..4252432 [+], 1035500091586DUF2169 domain-containing protein 
13AZO_RS209654252437..4253474 [+], 1038500091587hypothetical protein 
14AZO_RS194654253497..4254504 [+], 1008500091588AHH domain-containing protein 
15AZO_RS203604254527..4255069 [+], 543500091589hypothetical protein 
16AZO_RS194754255159..4255536 [+], 378500091590DUF4150 domain-containing protein 
17AZO_RS194804255546..4255974 [+], 429500091591hypothetical protein 
18AZO_RS194854255979..4257190 [+], 1212500091592hypothetical protein 
19AZO_RS19490 (tagH)4257210..4259045 [+], 1836500091593type VI secretion system-associated FHA domain protein TagH 
20AZO_RS194954259097..4259867 [+], 771500091594serine/threonine-protein phosphatase 
21AZO_RS195004260004..4260594 [+], 591753917957hypothetical protein 
22AZO_RS195054260635..4261894 [+], 1260500091596hypothetical protein 
23AZO_RS195104261940..4263946 [-], 2007500091597protein kinase 
24AZO_RS19515 (tagF)4264005..4264712 [-], 7081789762922type VI secretion system-associated protein TagF 
25AZO_RS19520 (tssM)4264709..4268281 [-], 3573500091599type VI secretion system membrane subunit TssM 
26AZO_RS195254268313..4269617 [-], 1305500091600DotU family type VI secretion system protein 
27AZO_RS19530 (tssK)4269682..4271016 [-], 1335500091601type VI secretion system baseplate subunit TssK 
28AZO_RS19535 (tssJ)4271065..4271583 [-], 519500091602type VI secretion system lipoprotein TssJ 
29AZO_RS19540 (tssA)4271852..4272913 [+], 1062500091603type VI secretion system protein TssA 
30AZO_RS19545 (tssB)4272962..4273471 [+], 510500091604type VI secretion system contractile sheath small subunit 
31AZO_RS19550 (tssC)4273523..4275019 [+], 1497500091605type VI secretion system contractile sheath large subunit 
32AZO_RS195554275148..4275636 [+], 489500091606type VI secretion system tube protein Hcp 
33AZO_RS195604275831..4276337 [+], 507500091607type VI secretion system tube protein Hcp 
34AZO_RS195654276353..4277174 [+], 822500091608tetratricopeptide repeat protein 
35AZO_RS19570 (tssE)4277194..4277676 [+], 4831948757278type VI secretion system baseplate subunit TssE 
36AZO_RS19575 (tssF)4277775..4279646 [+], 1872500091610type VI secretion system baseplate subunit TssF 
37AZO_RS19580 (tssG)4279622..4280665 [+], 1044500091611type VI secretion system baseplate subunit TssG 
38AZO_RS19585 (tssH)4280714..4283434 [+], 2721500091612type VI secretion system ATPase TssH 
39AZO_RS195904283535..4283924 [+], 390500091613hypothetical protein 
40AZO_RS195954283936..4285093 [+], 1158500091614lipase family protein 
41AZO_RS196004285131..4286267 [+], 1137500091615hypothetical protein 
42AZO_RS196054286286..4287635 [+], 1350500091616tetratricopeptide repeat protein 
43AZO_RS196104287636..4289066 [-], 1431500091617serine/threonine protein kinase 
44AZO_RS196154289077..4289949 [-], 873500091618DUF3365 domain-containing protein 
45AZO_RS196204290002..4290871 [-], 870753915808FecR domain-containing protein 
46AZO_RS196254291053..4292258 [+], 1206500091620beta-ketoacyl-[acyl-carrier-protein] synthase family protein 
47AZO_RS196304292263..4293090 [+], 828500091621beta-ketoacyl synthase chain length factor 
48AZO_RS196354293195..4293596 [+], 40219487572793-hydroxylacyl-ACP dehydratase 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

Download FASTA format files
Proteins        Genes
Regulatory element identified
azo3888ActivatorPMID: 22616609

(1) Shidore T et al. (2012). Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72. Environ Microbiol. 14(10):2775-87. [PudMed:22616609] experimental in_silico
(2) Boyer F et al. (2009). Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 10:104. [PudMed:19284603] in_silico
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation