I. Summary

SecReT6 ID	164
Strain	Acidovorax citrulli AAC00-1
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_008752
Location	1617808..1647773
Comment	T6SS plays a role in BFB seed-to-seedling transmission, melon seed colonization and biofilm formation
Function	anti-eukaryotic
T6SS Type	Type i; subtype i3
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	0	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	Aave_1462	1614190..1615446 [-], 1257	120610149	RND family efflux transporter MFP subunit
2	Aave_1463	1615495..1616532 [+], 1038	120610150	squalene/phytoene synthase
3	Aave_1464	1616619..1617287 [-], 669	120610151	hypothetical protein
4	Aave_1465	1617808..1618290 [-], 483	120610152	hypothetical protein	TssD
5	Aave_1466	1618859..1620844 [+], 1986	120610153	serine/threonine protein kinase
6	Aave_1467	1620951..1621451 [+], 501	120610154	hypothetical protein
7	Aave_1468	1621473..1623806 [+], 2334	120610155	FHA domain-containing protein	Fha
8	Aave_1469	1623842..1624591 [+], 750	120610156	protein serine/threonine phosphatase
9	Aave_1470	1624635..1625198 [+], 564	120610157	putative lipoprotein	TssJ
10	Aave_1471	1625240..1626574 [+], 1335	120610158	hypothetical protein	TssK
11	Aave_1472	1626583..1627941 [+], 1359	120610159	hypothetical protein	TssL
12	Aave_1473	1627945..1631574 [+], 3630	120610160	ImcF domain-containing protein	TssM
13	Aave_1474	1631556..1632269 [+], 714	120610161	hypothetical protein
14	Aave_1475	1632316..1633356 [+], 1041	120610162	ImpA domain-containing protein	TssA
15	Aave_1476	1633519..1634067 [+], 549	120610163	hypothetical protein	TssB
16	Aave_1477	1634081..1635580 [+], 1500	120610164	hypothetical protein	TssC
17	Aave_1478	1635633..1636490 [+], 858	120610165	virulence protein, SciE type	TagJ
18	Aave_1479	1636507..1637028 [+], 522	120610166	hypothetical protein	TssE
19	Aave_1480	1637101..1638975 [+], 1875	120610167	hypothetical protein	TssF
20	Aave_1481	1638972..1640138 [+], 1167	120610168	hypothetical protein	TssG
21	Aave_1482	1640211..1642988 [+], 2778	120610169	ATPase	TssH
22	Aave_1483	1643375..1644013 [+], 639	120610170	hypothetical protein
23	Aave_1484	1644040..1644546 [+], 507	120610171	hypothetical protein
24	Aave_1485	1644568..1646817 [-], 2250	120610172	xanthine dehydrogenase, molybdenum-binding subunit apoprotein
25	Aave_1486	1646823..1647773 [-], 951	120610173	molybdopterin dehydrogenase
26	Aave_1488	1648610..1649500 [-], 891	120610174	glutamate racemase
27	Aave_1489	1649597..1651156 [-], 1560	120610175	fumarase

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files

Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

BTH_I0068

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	Aave_1502	1666961..1667398 [-], 438	120610188	hypothetical protein	BTH_I0068

Download FASTA format files

Proteins        Genes

N/A

(1) Tian Y et al. (2014). The Type VI Protein Secretion System Contributes to Biofilm Formation and Seed-to-Seedling Transmission of Acidovorax citrulli on melon. Mol Plant Pathol. 16(1):38-47. [PudMed:24863458]

(2) Boyer F et al. (2009). Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 10:104. [PudMed:19284603]

This literature contains experimental investigation

This literature contains bioinformatics investigation