I. Summary
SecReT6 ID17 experimental
StrainYersinia pestis CO92
Repliconchromosome [Browse all T6SS(s) in this replicon]
CommentPhagocytosis-promoting activity in macrophage-like J774.A1 cells
Functionanti-eukaryotic; virulence
T6SS TypeType i; subtype i3
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1YPO0489521134..521598 [+], 465218927687hypothetical protein 
2YPO0490 (apaH)521688..522557 [-], 870218927688diadenosine tetraphosphatase 
3YPO0491 (apaG)522574..522951 [-], 378218927689ApaG 
4YPO0492 (ksgA)522965..523783 [-], 819218927690dimethyladenosine transferase 
5YPO0493 (pdxA)523776..524771 [-], 9962189276914-hydroxythreonine-4-phosphate dehydrogenase 
6YPO0494 (surA)524755..526059 [-], 1305218927692peptidyl-prolyl cis-trans isomerase SurA 
7YPO0495 (imp)526127..528469 [-], 2343218927693organic solvent tolerance protein 
8YPO0496 (djlA)528654..529487 [+], 834218927694Dna-J like membrane chaperone protein 
9YPO0497 (rluA)529785..530405 [-], 621218927695ribosomal large subunit pseudouridine synthase A 
10YPO0498531628..532371 [-], 744218927696hypothetical protein 
11YPO0499532701..533714 [+], 1014218927697hypothetical protein  TssA
12YPO0500533725..534285 [+], 561218927698hypothetical protein  TssB
13YPO0501534285..535796 [+], 1512218927699hypothetical protein  TssC
14YPO0502535959..536477 [+], 519218927700hypothetical protein  TssD
15YPO0503536551..536994 [+], 444218927701hypothetical protein  TssE
16YPO0504537027..538871 [+], 1845218927702hypothetical protein  TssF
17YPO0505538864..539847 [+], 984218927703hypothetical protein  TssG
18YPO0506 (clpB)539850..542453 [+], 2604218927704Clp ATPase  TssH
19YPO0507542557..544905 [+], 2349218927705hypothetical protein  TssI
20YPO0508544918..545490 [+], 573218927706hypothetical protein 
21YPO0509545515..547137 [+], 1623218927707hypothetical protein 
22YPO0510547163..548266 [+], 1104218927708hypothetical protein 
23YPO0511548259..548876 [+], 618218927709hypothetical protein 
24YPO0511a548882..549247 [+], 366218927710hypothetical protein  PAAR
25YPO0512549240..549731 [+], 492218927711lipoprotein  TssJ
26YPO0513549851..551206 [+], 1356218927712hypothetical protein  TssK
27YPO0514551203..552813 [+], 1611218927713hypothetical protein  TssL
28YPO0515552863..556315 [+], 3453218927714hypothetical protein  TssM
29YPO0516556337..556690 [+], 354218927715hypothetical protein 
30YPO0517 (hepA)556946..559852 [-], 2907218927716ATP-dependent helicase HepA 
31YPO0518 (dinA)560313..562682 [-], 2370218927717DNA polymerase II 
32YPO0519562878..563645 [+], 768218927718DedA family membrane protein 
33YPO0520 (thiQ)563714..564397 [-], 684218927719thiamine transporter ATP-binding subunit 
34YPO0521 (thiP)564411..565970 [-], 1560218927720thiamine transporter membrane protein 
35YPO0522 (tbpA)565994..566986 [-], 993218927721thiamine transporter substrate binding subunit 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
YPK_3563 , YPK_3558

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1YPO0502535959..536477 [+], 519218927700hypothetical protein YPK_3563
2YPO0507542557..544905 [+], 2349218927705hypothetical protein YPK_3558

Download FASTA format files
Proteins        Genes
Regulatory element identified
RovA (YPO2374)Indirect activatorPMID: 16938880

(1) Robinson JB et al. (2009). Evaluation of a Yersinia pestis mutant impaired in a thermoregulated type VI-like secretion system in flea, macrophage and murine models. Microb Pathog. 47(5):243-51. [PudMed:19716410] experimental
(2) Boyer F et al. (2009). Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources. BMC Genomics. 10:104. [PudMed:19284603] in_silico
(3) Cathelyn JS et al. (2006). RovA, a global regulator of Yersinia pestis, specifically required for bubonic plague. Proc Natl Acad Sci U S A. 103(36):13514-9. [PudMed:16938880] experimental
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation