I. Summary

SecReT6 ID	178
Strain	Burkholderia pseudomallei 668
Replicon	chromosome I [Browse all T6SS(s) in this replicon]
Accession	NC_009074
Location	3546300..3566286
Comment
Function	N/A
T6SS Type	Type i; subtype i4b
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	0	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	BURPS668_3620	3542443..3543891 [+], 1449	284159962	TolC family type I secretion outer membrane protein
2	BURPS668_3621	3543888..3544025 [+], 138	126439355	hypothetical protein
3	BURPS668_3622	3544101..3545051 [-], 951	126442042	hypothetical protein
4	BURPS668_3623	3545063..3546028 [+], 966	126438736	D-alanyl-D-alanine carboxypeptidase
5	BURPS668_3624	3546179..3546292 [+], 114	126440299	hypothetical protein
6	BURPS668_3625	3546300..3550193 [+], 3894	126440145	hypothetical protein	TssM
7	BURPS668_3626	3550190..3551179 [+], 990	126439251	hypothetical protein
8	BURPS668_3627	3551184..3552116 [+], 933	126440077	ompA family protein	TagL
9	BURPS668_3628	3552315..3553436 [-], 1122	126441470	ImpA-related N-terminal family protein	TssA
10	BURPS668_3629	3553526..3556195 [-], 2670	126438408	ClpA/B type protease	TssH
11	BURPS668_3630	3556229..3557329 [-], 1101	126439645	hypothetical protein	TssG
12	BURPS668_3631	3557293..3559131 [-], 1839	126440612	hypothetical protein	TssF
13	BURPS668_3632	3559211..3559693 [-], 483	126441892	hypothetical protein	TssE
14	BURPS668_3633	3559751..3560254 [-], 504	126439263	hypothetical protein	TssD
15	BURPS668_3634	3560327..3561817 [-], 1491	126442286	hypothetical protein	TssC
16	BURPS668_3635	3561834..3562352 [-], 519	126440974	hypothetical protein	TssB
17	BURPS668_3636	3562389..3563024 [-], 636	126439636	hypothetical protein
18	BURPS668_3637	3563186..3563323 [-], 138	126438770	hypothetical protein
19	BURPS668_3638	3563435..3564049 [+], 615	126441329	lipoprotein	TssJ
20	BURPS668_3639	3564098..3565504 [+], 1407	126442128	hypothetical protein	TssK
21	BURPS668_3640	3565501..3566286 [+], 786	126441100	hypothetical protein	TssL
22	BURPS668_3641 (intB)	3566772..3567110 [-], 339	126440028	Phage integrase
23	BURPS668_3642	3569704..3569967 [+], 264	126440666	hypothetical protein
24	BURPS668_3643	3569991..3570824 [+], 834	126439324	TnpB

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

BTH_I2697 , BTH_I2705, BCAL0343, BTH_I0068, BTH_I2698, BTH_I2693, BTH_I3226, BTH_I3225, BTH_II0090, BTH_II0310

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	BURPS668_1585	1551963..1555298 [+], 3336	126438749	Rhs element Vgr protein	BTH_I2697
2	BURPS668_1580	1544522..1547329 [+], 2808	126439104	Rhs element Vgr protein	BTH_I2705
3	BURPS668_3633	3559751..3560254 [-], 504	126439263	hypothetical protein	BCAL0343
4	BURPS668_0080	75977..76417 [+], 441	126439768	hypothetical protein	BTH_I0068
5	BURPS668_1584	1549545..1551854 [+], 2310	126441542	hypothetical protein	BTH_I2698
6	BURPS668_1590	1557430..1560270 [+], 2841	126441823	Rhs element Vgr protein	BTH_I2693
7	BURPS668_A0128	111023..113263 [+], 2241	126443653	hypothetical protein	BTH_I3226
8	BURPS668_A0127	108223..111009 [+], 2787	126444328	putative Rhs element Vgr protein	BTH_I3225
9	BURPS668_A1695	1646115..1647458 [-], 1344	126444360	Type V secretory pathway, adhesin AidA	BTH_II0090
10	BURPS668_A2913	2765611..2766516 [+], 906	126444367	outer membrane protein	BTH_II0310

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Proteins        Genes

Homology Immunity protein list

BTH_I2700, BTH_I0069

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	BURPS668_1582	1548350..1548859 [+], 510	126440343	hypothetical protein	BTH_I2700
2	BURPS668_0081	76404..76772 [+], 369	126442147	lipoprotein	BTH_I0069

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Proteins        Genes

N/A