I. Summary

SecReT6 ID	217
Strain	Pseudomonas aeruginosa PA7
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_009656
Location	161170..187540
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	2	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	PSPA7_0145	156649..159747 [-], 3099	152984686	serine/threonine protein kinase PpkA	PpkA
2	PSPA7_0146	159755..160483 [-], 729	152986292	putative phosphoprotein phosphatase	PppA
3	PSPA7_0147	160493..161173 [-], 681	152987384	hypothetical protein	TagF
4	PSPA7_0148	161170..164700 [-], 3531	152984454	hypothetical protein	TssM
5	PSPA7_0149	164697..166046 [-], 1350	152985769	hypothetical protein	TssL
6	PSPA7_0150	166053..167387 [-], 1335	152988561	hypothetical protein	TssK
7	PSPA7_0151	167404..167868 [-], 465	152985140	putative lipoprotein	TssJ
8	PSPA7_0152	167913..169439 [-], 1527	152985795	hypothetical protein	Fha
9	PSPA7_0153	169449..169592 [+], 144	152989219	hypothetical protein
10	PSPA7_0154	169807..170838 [+], 1032	152984264	hypothetical protein	TssA
11	PSPA7_0155	170927..171445 [+], 519	152983480	hypothetical protein	TssB
12	PSPA7_0156	171458..172954 [+], 1497	152984184	hypothetical protein	TssC
13	PSPA7_0157	173030..173518 [+], 489	152985902	hypothetical protein	TssD
14	PSPA7_0158	173677..174462 [+], 786	152988565	virulence protein, SciE type	TagJ
15	PSPA7_0159	174464..174973 [+], 510	152984195	hypothetical protein	TssE
16	PSPA7_0160	174970..176829 [+], 1860	152988188	hypothetical protein	TssF
17	PSPA7_0161	176793..177839 [+], 1047	152986386	hypothetical protein	TssG
18	PSPA7_0162	177832..180540 [+], 2709	152989614	type VI secretion ATPase	TssH
19	PSPA7_0163	180575..182515 [+], 1941	152987570	hypothetical protein	TssI
20	PSPA7_0164	182522..182635 [-], 114	152987514	hypothetical protein
21	PSPA7_0165	182642..182767 [-], 126	152986670	hypothetical protein
22	PSPA7_0166	182778..183311 [-], 534	152986602	hypothetical protein
23	PSPA7_0167	183308..184612 [-], 1305	152985744	PAAR motif-containing protein	Tse6 (e)
24	PSPA7_0168	184628..185062 [-], 435	152987036	hypothetical protein
25	PSPA7_0169	185315..187540 [+], 2226	152983873	hypothetical protein	TssI
26	PSPA7_0170	187569..188018 [+], 450	152986466	hypothetical protein
27	PSPA7_0171	188035..189147 [+], 1113	152985103	hypothetical protein
28	PSPA7_0172	189144..190181 [+], 1038	152986846	3-oxoacyl-(acyl carrier protein) synthase
29	PSPA7_0173	190181..191344 [+], 1164	152989562	hypothetical protein
30	PSPA7_0174	191721..192281 [+], 561	152984217	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PA0095 , PA2702, PA2684, PA0093, PA3484, PA0085, PA2685, PA1844, PA0091

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PSPA7_0169	185315..187540 [+], 2226	152983873	hypothetical protein	PA0095
2	PSPA7_3979	4123474..4123950 [+], 477	152984462	hypothetical protein	PA2702
3	PSPA7_3959	4099703..4103662 [-], 3960	152985313	hypothetical protein	PA2684
4	PSPA7_0167	183308..184612 [-], 1305	152985744	PAAR motif-containing protein	PA0093
5	PSPA7_1642	1679339..1680565 [-], 1227	152985781	hypothetical protein	PA3484
6	PSPA7_0157	173030..173518 [+], 489	152985902	hypothetical protein	PA0085
7	PSPA7_3960	4103712..4105781 [-], 2070	152985978	hypothetical protein	PA2685
8	PSPA7_3448	3574266..3574736 [+], 471	152987248	hypothetical protein	PA1844
9	PSPA7_0163	180575..182515 [+], 1941	152987570	hypothetical protein	PA0091

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Proteins        Genes

Homology Immunity protein list

Tsi5, PA2703, PA3485, PA1845

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PSPA7_3958	4099515..4099706 [-], 192	152983834	hypothetical protein	Tsi5
2	PSPA7_3980	4123960..4124193 [+], 234	152988085	hypothetical protein	PA2703
3	PSPA7_1641	1678905..1679342 [-], 438	152988990	hypothetical protein	PA3485
4	PSPA7_3447	3573704..3574222 [+], 519	152989292	hypothetical protein	PA1845

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Proteins        Genes

(1) Barret M et al. (2011). Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537]

This literature contains bioinformatics investigation