I. Summary

SecReT6 ID	225
Strain	Yersinia pseudotuberculosis IP 31758
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_009708
Location	3850633..3874248
Comment	T6SS-4 promotes Y. pseudotuberculosis survival in high osmolarity conditions and resistance to deoxycholate
Function	N/A
T6SS Type	Type i; subtype i3
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	YpsIP31758_3419 (rapA)	3847096..3850002 [+], 2907	153949731	ATP-dependent helicase HepA
2	YpsIP31758_3420	3850258..3850611 [-], 354	153948883	hypothetical protein
3	YpsIP31758_3421	3850633..3854127 [-], 3495	153949294	hypothetical protein	TssM
4	YpsIP31758_3422	3854136..3855746 [-], 1611	153950101	hypothetical protein	TssL
5	YpsIP31758_3423	3855743..3857098 [-], 1356	153950497	hypothetical protein	TssK
6	YpsIP31758_3424	3857218..3857709 [-], 492	153948671	lipoprotein	TssJ
7	YpsIP31758_3425	3857702..3858067 [-], 366	153948858	hypothetical protein	PAAR
8	YpsIP31758_3426	3858073..3858690 [-], 618	153947713	hypothetical protein
9	YpsIP31758_3427	3858683..3859786 [-], 1104	153948573	pentapeptide repeat-containing protein
10	YpsIP31758_3428	3859812..3862031 [-], 2220	153950018	pentapeptide repeat-containing protein
11	YpsIP31758_3429	3862044..3864392 [-], 2349	153947514	Rhs element Vgr protein	TssI
12	YpsIP31758_3430	3864496..3867099 [-], 2604	153949405	AAA ATPase	TssH
13	YpsIP31758_3431	3867102..3868085 [-], 984	153948961	hypothetical protein	TssG
14	YpsIP31758_3432	3868078..3869922 [-], 1845	153948697	hypothetical protein	TssF
15	YpsIP31758_3433	3869955..3870398 [-], 444	153948252	hypothetical protein	TssE
16	YpsIP31758_3434	3870472..3870990 [-], 519	153948599	hypothetical protein	TssD
17	YpsIP31758_3435	3871153..3872655 [-], 1503	153949071	hypothetical protein	TssC
18	YpsIP31758_3436	3872664..3873224 [-], 561	153948157	hypothetical protein	TssB
19	YpsIP31758_3437	3873235..3874248 [-], 1014	153948721	ImpA domain-containing protein	TssA
20	YpsIP31758_3438	3874578..3875321 [+], 744	153946848	hypothetical protein
21	YpsIP31758_3439 (rluA)	3876544..3877164 [+], 621	153948334	ribosomal large subunit pseudouridine synthase A
22	YpsIP31758_3440 (djlA)	3877481..3878314 [-], 834	153950401	Dna-J like membrane chaperone protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

YPK_3558 , YPK_3563

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	YpsIP31758_3429	3862044..3864392 [-], 2349	153947514	Rhs element Vgr protein	YPK_3558
2	YpsIP31758_3434	3870472..3870990 [-], 519	153948599	hypothetical protein	YPK_3563

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Proteins        Genes

N/A

Regulatory element identified

OmpR

Regulator	Description	Reference
OmpR (YpsIP31758_3980)	Activator	PMID: 23840509

lptD

Regulator	Description	Reference
lptD (YpsIP31758_3441)	Activator	PMID: 23840509

(1) Gueguen E et al. (2013). Expression of a Yersinia pseudotuberculosis Type VI Secretion System Is Responsive to Envelope Stresses through the OmpR Transcriptional Activator. PLoS One. 8(6):e66615. [PudMed:23840509]

This literature contains experimental investigation