I. Summary

SecReT6 ID	225
Strain	Yersinia pseudotuberculosis IP 31758
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_009708
Location	3850633..3874248
Comment	T6SS-4 promotes Y. pseudotuberculosis survival in high osmolarity conditions and resistance to deoxycholate
Function	N/A
T6SS Type	Type i; subtype i3
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	YPSIP31758_RS18425 (rapA)	3847096..3850002 [+], 2907	499505079	RNA polymerase-associated protein RapA
2	YPSIP31758_RS18430	3850258..3850611 [-], 354	488139260	hypothetical protein
3	YPSIP31758_RS18435 (tssM)	3850633..3854074 [-], 3442	pseudogene	type VI secretion system membrane subunit TssM
4	YPSIP31758_RS18440	3854136..3855746 [-], 1611	499505078	type IVB secretion system protein IcmH/DotU
5	YPSIP31758_RS18445 (tssK)	3855743..3857098 [-], 1356	488139262	type VI secretion system baseplate subunit TssK
6	YPSIP31758_RS18450 (tssJ)	3857218..3857709 [-], 492	499505077	type VI secretion system lipoprotein TssJ
7	YPSIP31758_RS18455	3857702..3858067 [-], 366	488143529	DUF4150 domain-containing protein
8	YPSIP31758_RS18460	3858073..3858690 [-], 618	488139265	DUF3540 domain-containing protein
9	YPSIP31758_RS18465	3858683..3859786 [-], 1104	488143531	pentapeptide repeat-containing protein
10	YPSIP31758_RS18470	3859812..3862031 [-], 2220	488152879	pentapeptide repeat-containing protein
11	YPSIP31758_RS18475 (vgrG)	3862044..3864392 [-], 2349	501054164	type VI secretion system tip protein VgrG
12	YPSIP31758_RS18480 (tssH)	3864496..3867084 [-], 2589	488139267	type VI secretion system ATPase TssH
13	YPSIP31758_RS18485 (tssG)	3867102..3868085 [-], 984	501054165	type VI secretion system baseplate subunit TssG
14	YPSIP31758_RS18490 (tssF)	3868078..3869922 [-], 1845	499505074	type VI secretion system baseplate subunit TssF
15	YPSIP31758_RS18495	3869955..3870398 [-], 444	488139270	type VI secretion system baseplate subunit TssE
16	YPSIP31758_RS18500	3870472..3870990 [-], 519	488139271	type VI secretion system tube protein Hcp
17	YPSIP31758_RS18505 (tssC)	3871153..3872655 [-], 1503	488139272	type VI secretion system contractile sheath large subunit
18	YPSIP31758_RS18510 (tssB)	3872664..3873224 [-], 561	488139273	type VI secretion system contractile sheath small subunit
19	YPSIP31758_RS18515	3873235..3874248 [-], 1014	488139274	ImpA family type VI secretion system protein
20	YPSIP31758_RS18520	3874578..3875321 [+], 744	488143539	hexameric transcriptional regulator RovC
21	YPSIP31758_RS18525 (rluA)	3876544..3877164 [+], 621	488139277	bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA
22	YPSIP31758_RS18530 (djlA)	3877481..3878314 [-], 834	499505073	co-chaperone DjlA

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

YPK_3558 , YPK_3563

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog

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Proteins        Genes

N/A

Regulatory element identified

OmpR

Regulator	Description	Reference
OmpR (YpsIP31758_3980)	Activator	PMID: 23840509

lptD

Regulator	Description	Reference
lptD (YpsIP31758_3441)	Activator	PMID: 23840509

(1) Gueguen E et al. (2013). Expression of a Yersinia pseudotuberculosis Type VI Secretion System Is Responsive to Envelope Stresses through the OmpR Transcriptional Activator. PLoS One. 8(6):e66615. [PudMed:23840509]

This literature contains experimental investigation