I. Summary

SecReT6 ID	277
Strain	Yersinia pseudotuberculosis PB1/+
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_010634
Location	740987..764575
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

13

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	YPTS_0660 (djlA)	736894..737727 [+], 834	186893989	Dna-J like membrane chaperone protein
2	YPTS_0661	738044..738664 [-], 621	186893990	pseudouridine synthase
3	YPTS_0662	739887..740630 [-], 744	186893991	hypothetical protein
4	YPTS_0663	740987..741973 [+], 987	186893992	ImpA domain-containing protein	TssA
5	YPTS_0664	741984..742544 [+], 561	186893993	type VI secretion protein	TssB
6	YPTS_0665	742544..744055 [+], 1512	186893994	EvpB family type VI secretion protein	TssC
7	YPTS_0666	744218..744736 [+], 519	186893995	hypothetical protein	TssD
8	YPTS_0667	744810..745253 [+], 444	186893996	type VI secretion system lysozyme-like protein	TssE
9	YPTS_0668	745286..747130 [+], 1845	186893997	type VI secretion protein	TssF
10	YPTS_0669	747123..748106 [+], 984	186893998	type VI secretion protein	TssG
11	YPTS_0670	748124..750712 [+], 2589	186893999	type VI secretion ATPase	TssH
12	YPTS_0671	750816..753164 [+], 2349	186894000	ImpA family type VI secretion-associated protein	TssI
13	YPTS_0672	753177..755396 [+], 2220	186894001	pentapeptide repeat-containing protein
14	YPTS_0673	755422..756525 [+], 1104	186894002	pentapeptide repeat-containing protein
15	YPTS_0674	756518..757135 [+], 618	186894003	hypothetical protein
16	YPTS_0675	757141..757506 [+], 366	186894004	hypothetical protein	PAAR
17	YPTS_0676	757499..757990 [+], 492	186894005	putative lipoprotein	TssJ
18	YPTS_0677	758110..759465 [+], 1356	186894006	type VI secretion protein	TssK
19	YPTS_0678	759462..761072 [+], 1611	186894007	hypothetical protein	TssL
20	YPTS_0679	761081..764575 [+], 3495	186894008	type VI secretion protein IcmF	TssM
21	YPTS_0680	764597..764950 [+], 354	186894009	hypothetical protein
22	YPTS_0681	765098..765244 [+], 147	186894010	hypothetical protein
23	YPTS_0682	765206..768112 [-], 2907	186894011	ATP-dependent helicase HepA

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

YPK_3563 , YPK_3558

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	YPTS_0666	744218..744736 [+], 519	186893995	hypothetical protein	YPK_3563
2	YPTS_0671	750816..753164 [+], 2349	186894000	ImpA family type VI secretion-associated protein	YPK_3558

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Proteins        Genes

N/A

N/A