I. Summary

SecReT6 ID	284
Strain	Burkholderia phytofirmans PsJN
Replicon	chromosome I [Browse all T6SS(s) in this replicon]
Accession	NC_010681
Location	1048323..1068598
Comment
Function	N/A
T6SS Type	Type i; subtype i3
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	0	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

12

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	Bphyt_0929	1043465..1043770 [-], 306	187922930	urease subunit beta
2	Bphyt_0930 (ureA)	1043786..1044088 [-], 303	187922931	urease subunit gamma
3	Bphyt_0931	1044264..1045067 [-], 804	187922932	family 3 extracellular solute-binding protein
4	Bphyt_0932	1045319..1046404 [-], 1086	187922933	hypothetical protein
5	Bphyt_0933	1046459..1048156 [-], 1698	187922934	hypothetical protein
6	Bphyt_0934	1048323..1052312 [-], 3990	187922935	type VI secretion protein IcmF	TssM
7	Bphyt_0935	1052341..1053585 [-], 1245	187922936	hypothetical protein	TssL
8	Bphyt_0936	1053731..1055074 [-], 1344	187922937	type VI secretion protein	TssK
9	Bphyt_0937	1055087..1055599 [-], 513	187922938	type VI secretion lipoprotein	TssJ
10	Bphyt_0938	1055671..1056153 [-], 483	187922939	hypothetical protein	TssD
11	Bphyt_0939	1056373..1056804 [-], 432	187922940	hypothetical protein
12	Bphyt_0940	1056906..1058405 [-], 1500	187922941	type VI secretion protein EvpB	TssC
13	Bphyt_0941	1058476..1059021 [-], 546	187922942	type VI secretion protein	TssB
14	Bphyt_0942	1059058..1061778 [-], 2721	187922943	type VI secretion ATPase, ClpV1 family	TssH
15	Bphyt_0943	1062472..1063080 [+], 609	187922944	hypothetical protein
16	Bphyt_0944	1063077..1063949 [+], 873	187922945	virulence protein SciE type	TagJ
17	Bphyt_0945	1063946..1064482 [+], 537	187922946	type VI secretion system lysozyme-like protein	TssE
18	Bphyt_0946	1064498..1066387 [+], 1890	187922947	type VI secretion protein	TssF
19	Bphyt_0947	1066387..1067499 [+], 1113	187922948	type VI secretion protein	TssG
20	Bphyt_0948	1067492..1068598 [+], 1107	187922949	ImpA domain-containing protein	TssA
21	Bphyt_0949	1068876..1069757 [-], 882	187922950	urease accessory protein UreD
22	Bphyt_0950	1069875..1070567 [-], 693	187922951	urea ABC transporter ATP-binding protein
23	Bphyt_0951	1070600..1071466 [-], 867	187922952	urea ABC transporter ATP-binding protein
24	Bphyt_0952	1071463..1072647 [-], 1185	187922953	urea ABC transporter permease

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

ROD_27741

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	Bphyt_0938	1055671..1056153 [-], 483	187922939	hypothetical protein	ROD_27741

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Proteins        Genes

N/A

N/A