I. Summary
SecReT6 ID292 experimental
StrainBurkholderia cenocepacia J2315
Repliconchromosome I [Browse all T6SS(s) in this replicon]
CommentResistance to Dictyostelium predation; virulence in rat model of chronic lung infection; induction of cytoskeletal rearrangements in cultured macrophages; inflammasome activation.
B. cenocepacia K56-2: clonally related to J2315, CF clinical Isolate
Functionanti-eukaryotic; effector translocation;virulence
T6SS TypeType i; subtype i4b
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1BCAL0330 (petC)358266..359024 [+], 759206558736cytochrome c1 
2BCAL0331359119..359730 [+], 612206558737putative stringent starvation protein A 
3BCAL0332359799..360323 [+], 525206558738ClpXP protease specificity-enhancing factor 
4BCAL0333360772..360975 [+], 204206558739hypothetical protein 
5BCAL0334361085..361885 [-], 801206558740periplasmic solute-binding protein 
6BCAL0335362167..362484 [+], 318206558741hypothetical protein 
7BCAL0336362566..362901 [+], 336206558742hypothetical protein 
8BCAL0337363015..363797 [-], 783206558743putative type VI secretion system protein TssL  TssL
9BCAL0338363794..365140 [-], 1347206558744putative type VI secretion system protein TssK  TssK
10BCAL0339365246..365845 [-], 600206558745putative type VI secretion system protein TssJ  TssJ
11BCAL0340366232..366858 [+], 627206558746putative lipoprotein 
12BCAL0341366905..367420 [+], 516206558747putative type VI secretion system protein TssB  TssB
13BCAL0342367436..368926 [+], 1491206558748putative type VI secretion system protein TssC  TssC
14BCAL0343368997..369500 [+], 504206558749putative type VI secretion system protein TssD  TssD
15BCAL0344369563..370048 [+], 486206558750putative type VI secretion system protein TssE  TssE
16BCAL0345370125..371960 [+], 1836206558751putative type VI secretion system protein TssF  TssF
17BCAL0346371924..373024 [+], 1101206558752putative type VI secretion system protein TssG  TssG
18BCAL0347 (clpB)373066..375735 [+], 2670206558753putative type VI secretion system protein TssH  TssH
19BCAL0348375780..376901 [+], 1122206558754putative type VI secretion system protein TssA  TssA
20BCAL0349376995..377945 [-], 951206558755putative outer membrane protein  TagL
21BCAL0350377950..378939 [-], 990206558756hypothetical protein 
22BCAL0351378936..382880 [-], 3945206558757putative type VI secretion system protein TssM  TssM
23BCAL0352383182..384027 [-], 846206558758subfamily M15C metalopeptidase 
24BCAL0353384149..385141 [+], 993206558759hypothetical protein 
25BCAL0354385353..386381 [+], 1029206558760putative cation transporter 
26BCAL0356386586..387563 [-], 978206558761putative quinone oxidoreductase 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
BCAL0343 , BTH_I2705, BTH_I2698, BTH_I2697, BTH_I2693

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1BCAL0343368997..369500 [+], 504206558749putative type VI secretion system protein TssD BCAL0343
2BCAL13551484514..1487309 [+], 2796206559721hypothetical protein BTH_I2705
3BCAL13581488856..1491255 [+], 2400206559724hypothetical protein BTH_I2698
4BCAL13591491317..1495018 [+], 3702206559725hypothetical protein BTH_I2697
5BCAL13621496284..1499088 [+], 2805206559727hypothetical protein BTH_I2693
Data from experimental literature

Download FASTA format files
Proteins        Genes
Regulatory element identified
AtsR (BCAM0379)RepressorPMID: 22830644

suhB (BCAL2157)ActivatorPMID: 22767545

AtsT (BCAM0381)RepressorPMID: 24014026

CepR (BCAM1868)ActivatorPMID: 19761612

CciR (BCAM0240)RepressorPMID: 19761612

(1) Xu H et al. (2014). Innate immune sensing of bacterial modifications of Rho GTPases by the Pyrin inflammasome. Nature. 513(7517):237-41. [PudMed:24919149] experimental
(2) Khodai-Kalaki M et al. (2013). Characterization of the AtsR hybrid sensor kinase phosphorelay pathway and identification of its response regulator in Burkholderia cenocepacia. J Biol Chem. 288(42):30473-84. [PudMed:24014026] experimental
(3) Rosales-Reyes R et al. (2012). Burkholderia cenocepacia Type VI Secretion System Mediates Escape of Type II Secreted Proteins into the Cytoplasm of Infected Macrophages. PLoS One. 7(7):e41726. [PudMed:22848580] experimental
(4) Aubert DF et al. (2013). The Burkholderia cenocepacia sensor kinase hybrid AtsR is a global regulator modulating quorum-sensing signalling. Environ Microbiol. 15(2):372-85. [PudMed:22830644] experimental
(5) Rosales-Reyes R et al. (2012). The suhB gene of Burkholderia cenocepacia is required for protein secretion, biofilm formation, motility and polymyxin B resistance. Microbiology. 158(Pt 9):2315-24. [PudMed:22767545] experimental
(6) Tolman JS et al. (2012). Global changes in gene expression by the opportunistic pathogen Burkholderia cenocepacia in response to internalization by murine macrophages. BMC Genomics. 13:63. [PudMed:22321740] experimental
(7) Flannagan RS et al. (2012). Burkholderia cenocepacia disrupts host cell actin cytoskeleton by inactivating Rac and Cdc42. Cell Microbiol. 14(2):239-54. [PudMed:22023324] experimental
(8) Aubert D et al. (2010). BcsKC is an essential protein for the type VI secretion system activity in Burkholderia cenocepacia that forms an outer membrane complex with BcsLB. J Biol Chem. 285(46):35988-98. [PudMed:20729192]
(9) O'Grady EP et al. (2009). Reciprocal regulation by the CepIR and CciIR quorum sensing systems in Burkholderia cenocepacia. BMC Genomics. 10:441. [PudMed:19761612] experimental
experimental This literature contains experimental investigation