I. Summary
SecReT6 ID292 experimental
StrainBurkholderia cenocepacia J2315
Repliconchromosome I [Browse all T6SS(s) in this replicon]
CommentResistance to Dictyostelium predation; virulence in rat model of chronic lung infection; induction of cytoskeletal rearrangements in cultured macrophages; inflammasome activation.
B. cenocepacia K56-2: clonally related to J2315, CF clinical Isolate
Functionanti-eukaryotic; effector translocation;virulence
T6SS TypeType i; subtype i4b
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1QU43_RS38180358266..359024 [+], 759493523029cytochrome c1 
2QU43_RS38185359119..359730 [+], 612493445191glutathione S-transferase N-terminal domain-containing protein 
3QU43_RS38190359802..360323 [+], 522493531328ClpXP protease specificity-enhancing factor 
4QU43_RS38200360772..360975 [+], 204493531325hypothetical protein 
5QU43_RS38205361085..361885 [-], 801493531337transporter substrate-binding domain-containing protein 
6QU43_RS38210362167..362484 [+], 318493531338hypothetical protein 
7QU43_RS38215362566..362901 [+], 336493531334hypothetical protein 
8QU43_RS38220363015..363797 [-], 783493537971type IVB secretion system protein IcmH/DotU  TssL
9QU43_RS38225 (tssK)363794..365140 [-], 1347493523019type VI secretion system baseplate subunit TssK  TssK
10QU43_RS38230 (tssJ)365246..365845 [-], 600493531335type VI secretion system lipoprotein TssJ  TssJ
11QU43_RS38235366232..366858 [+], 627501468864hypothetical protein 
12QU43_RS38240 (tssB)366905..367420 [+], 516493530383type VI secretion system contractile sheath small subunit  TssB
13QU43_RS38245 (tssC)367436..368926 [+], 1491493530385type VI secretion system contractile sheath large subunit  TssC
14QU43_RS38250368997..369500 [+], 504493523014type VI secretion system tube protein Hcp  TssD
15QU43_RS38255 (tssE)369563..370048 [+], 486493523013type VI secretion system baseplate subunit TssE  TssE
16QU43_RS38260 (tssF)370125..371960 [+], 1836493530384type VI secretion system baseplate subunit TssF  TssF
17QU43_RS38265 (tssG)371924..373024 [+], 1101493530382type VI secretion system baseplate subunit TssG  TssG
18QU43_RS38270 (tssH)373066..375735 [+], 2670493530380type VI secretion system ATPase TssH  TssH
19QU43_RS38275 (tssA)375780..376901 [+], 1122493530386type VI secretion system protein TssA  TssA
20QU43_RS38280376995..377945 [-], 951493540541OmpA family protein  TagL
21QU43_RS38285 (tagF)377950..378939 [-], 990493529227type VI secretion system-associated protein TagF 
22QU43_RS38290 (tssM)378936..382880 [-], 3945501468865type VI secretion system membrane subunit TssM  TssM
23QU43_RS38295383182..384027 [-], 846493535018M15 family metallopeptidase 
24QU43_RS38300384149..385141 [+], 993501468866hypothetical protein 
25QU43_RS38305385353..386381 [+], 1029493527894transporter 
26QU43_RS73250386409..386561 [-], 153493527887DUF1427 family protein 
27QU43_RS38310386586..387563 [-], 978501468867zinc-binding alcohol dehydrogenase family protein 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
BCAL0343 , BTH_I2705, BTH_I2698, BTH_I2697, BTH_I2693

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog

Download FASTA format files
Proteins        Genes
Regulatory element identified
AtsR (BCAM0379)RepressorPMID: 22830644

suhB (BCAL2157)ActivatorPMID: 22767545

AtsT (BCAM0381)RepressorPMID: 24014026

CepR (BCAM1868)ActivatorPMID: 19761612

CciR (BCAM0240)RepressorPMID: 19761612

(1) Xu H et al. (2014). Innate immune sensing of bacterial modifications of Rho GTPases by the Pyrin inflammasome. Nature. 513(7517):237-41. [PudMed:24919149] experimental
(2) Khodai-Kalaki M et al. (2013). Characterization of the AtsR hybrid sensor kinase phosphorelay pathway and identification of its response regulator in Burkholderia cenocepacia. J Biol Chem. 288(42):30473-84. [PudMed:24014026] experimental
(3) Rosales-Reyes R et al. (2012). Burkholderia cenocepacia Type VI Secretion System Mediates Escape of Type II Secreted Proteins into the Cytoplasm of Infected Macrophages. PLoS One. 7(7):e41726. [PudMed:22848580] experimental
(4) Aubert DF et al. (2013). The Burkholderia cenocepacia sensor kinase hybrid AtsR is a global regulator modulating quorum-sensing signalling. Environ Microbiol. 15(2):372-85. [PudMed:22830644] experimental
(5) Rosales-Reyes R et al. (2012). The suhB gene of Burkholderia cenocepacia is required for protein secretion, biofilm formation, motility and polymyxin B resistance. Microbiology. 158(Pt 9):2315-24. [PudMed:22767545] experimental
(6) Tolman JS et al. (2012). Global changes in gene expression by the opportunistic pathogen Burkholderia cenocepacia in response to internalization by murine macrophages. BMC Genomics. 13:63. [PudMed:22321740] experimental
(7) Flannagan RS et al. (2012). Burkholderia cenocepacia disrupts host cell actin cytoskeleton by inactivating Rac and Cdc42. Cell Microbiol. 14(2):239-54. [PudMed:22023324] experimental
(8) Aubert D et al. (2010). BcsKC is an essential protein for the type VI secretion system activity in Burkholderia cenocepacia that forms an outer membrane complex with BcsLB. J Biol Chem. 285(46):35988-98. [PudMed:20729192]
(9) O'Grady EP et al. (2009). Reciprocal regulation by the CepIR and CciIR quorum sensing systems in Burkholderia cenocepacia. BMC Genomics. 10:441. [PudMed:19761612] experimental
experimental This literature contains experimental investigation