I. Summary
SecReT6 ID3 experimental
StrainPseudomonas aeruginosa PAO1
Repliconchromosome (H1-T6SS) [Browse all T6SS(s) in this replicon]
CommentVirulence in rat model of chronic respiratory infection; Ability to outcompete a susceptible mutant of P. aeruginosa; ability to outcompete P. putida; growth inhibition of E. coli
Functionanti-bacterial and anti-eukaryotic; effector translocation;virulence
T6SS TypeType i; subtype i3
experimental Experimental investigation has been performed on this T6SS.

II. T6SS components
III. genome coordinates of the T6SS gene cluster
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductNote
1PA0067 (prlC)78710..80755 [+], 204615595265oligopeptidase A 
2PA006880752..81027 [+], 27615595266hypothetical protein 
3PA006981116..82174 [+], 105915595267hypothetical protein 
4PA007082404..83318 [-], 91515595268hypothetical protein  TagQ
5PA007183380..85092 [-], 171315595269hypothetical protein  TagR
6PA007285085..86284 [-], 120015595270hypothetical protein  TagS
7PA007386284..87003 [-], 72015595271ABC transporter ATP-binding protein  TagT
8PA0074 (ppkA)87000..90098 [-], 309915595272serine/threonine protein kinase PpkA  PpkA
9PA0075 (pppA)90106..90834 [-], 72915595273PppA  PppA
10PA007690844..91524 [-], 68115595274hypothetical protein  TagF
11PA0077 (icmF1)91521..94826 [-], 330615595275IcmF1  TssM
12PA007895048..96397 [-], 135015595276hypothetical protein  TssL
13PA007996404..97738 [-], 133515595277hypothetical protein  TssK
14PA008097754..98218 [-], 46515595278hypothetical protein  TssJ
15PA0081 (fha1)98263..99756 [-], 149415595279Fha1  Fha
16PA0082100124..101158 [+], 103515595280hypothetical protein  TssA
17PA0083101247..101765 [+], 51915595281hypothetical protein  TssB
18PA0084101778..103274 [+], 149715595282hypothetical protein  TssC
19PA0085 (hcp1)103350..103838 [+], 48915595283hypothetical protein  TssD
20PA0086104006..104851 [+], 84615595284hypothetical protein  TagJ
21PA0087104853..105362 [+], 51015595285hypothetical protein  TssE
22PA0088105359..107218 [+], 186015595286hypothetical protein  TssF
23PA0089107182..108228 [+], 104715595287hypothetical protein  TssG
24PA0090 (clpV1)108221..110929 [+], 270915595288ClpV1 protein  TssH
25PA0091 (vgrG1)110976..112907 [+], 193215595289VgrG1  TssI
26PA0092113022..113306 [-], 28515595290hypothetical protein  Tsi6 (i)
27PA0093113303..114595 [-], 129315595291hypothetical protein  Tse6 (e)
28PA0094114611..115045 [-], 43515595292hypothetical protein 
29PA0095115299..117524 [+], 222615595293hypothetical protein  TssI
30PA0096117552..118001 [+], 45015595294hypothetical protein 
31PA0097117931..119130 [+], 120015595295hypothetical protein 
32PA0098119127..120164 [+], 1038155952963-oxoacyl-ACP synthase 
33PA0099120164..121324 [+], 116115595297hypothetical protein  PA0099 (e)
34PA0100121346..122266 [+], 92115595298hypothetical protein 
35PA0101122248..123495 [+], 124815595299hypothetical protein 
36PA0102123871..124599 [+], 72915595300carbonic anhydrase 
37PA0103124810..126381 [+], 157215595301sulfate transporter 
38PA0104126518..127114 [+], 59715595302hypothetical protein 
flank Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

Download FASTA format files
Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.
1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Homology Effector list
PA0085 , PA0091, PA0093 (Tse6), PA0095, PA0099, PA1844 (Tse1, Tae1), PA2684 (Tse5), PA2685, PA2702 (Tse2), PA2774 (Tse4), PA3484 (Tse3, Tge1)

Effector identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA0085(hcp1)103350..103838 [+], 48915595283hypothetical protein PA0085
2PA0091(vgrG1)110976..112907 [+], 193215595289VgrG1 PA0091
3PA0093(Tse6)113303..114595 [-], 129315595291hypothetical protein (Tse6)PA0093 (Tse6)
4PA0095115299..117524 [+], 222615595293hypothetical protein PA0095
5PA0099120164..121324 [+], 116115595297hypothetical protein PA0099
6PA1844(Tse1)2003868..2004332 [-], 46515597041hypothetical protein (Tse1, Tae1)PA1844 (Tse1, Tae1)
7PA2684(Tse5)3031749..3035702 [-], 395415597880hypothetical protein (Tse5)PA2684 (Tse5)
8PA26853035752..3037932 [-], 218115597881hypothetical protein PA2685
9PA2702(Tse2)3056890..3057366 [+], 47715597898hypothetical protein (Tse2)PA2702 (Tse2)
10PA2774(Tse4)3132229..3132816 [-], 58815597970hypothetical protein (Tse4)PA2774 (Tse4)
11PA3484(Tse3)3898278..3899504 [+], 122715598680hypothetical protein (Tse3, Tge1)PA3484 (Tse3, Tge1)
Data from experimental literature

Download FASTA format files
Proteins        Genes

Homology Immunity protein list
PA0092 (Tsi6), PA1845 (Tsi1,Tai1), PA2703 (Tsi2), PA2775 (Tsi4), PA3485 (Tsi3,Tgi1)   PA2684.1 (Tsi5)

Immunity protein identified
#Locus tag(Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA0092113022..113306 [-], 28515595290hypothetical protein (Tsi6)PA0092 (Tsi6)
2PA1845(Tsi1)2004374..2004892 [-], 51915597042hypothetical protein (Tsi1,Tai1)PA1845 (Tsi1,Tai1)
3PA2703(Tsi2)3057376..3057609 [+], 23415597899hypothetical protein (Tsi2)PA2703 (Tsi2)
4PA27753132821..3133258 [-], 43815597971hypothetical protein (Tsi4)PA2775 (Tsi4)
5PA3485(Tsi3)3899501..3899938 [+], 43815598681hypothetical protein (Tsi3,Tgi1)PA3485 (Tsi3,Tgi1)
Data from experimental literature

Download FASTA format files
Proteins        Genes
Regulatory element identified
Gac/RsmIndirect activatorPMID: 21955777

LasR (PA1430)Indirect repressorPMID: 19497948

MvfR (PA1003)Indirect repressorPMID: 15686549

TagF (PA0076)RepressorPMID: 22017253

WspR (PA3702)ActivatorPMID: 21955777

Subinhibitory Level Kanamycin
Subinhibitory Level KanamycinIndirect activatorPMID: 24260549

PA2133RepressorPMID: 21955777

tpbB (PA1120)ActivatorPMID: 21955777

PpkA (PA0074)Activator: FhaPMID: 17558395

PppA (PA0075)Repressor: FhaPMID: 17558395

TagR (PA0071)Activator: ppkAPMID: 19400797

LadS (PA3974)ActivatorPMID: 16763151

RetS (PA4856)RepressorPMID: 16763151

AmpR (PA4109)RepressorPMID: 24291602

RsmY/RsmZ sRNAs
RsmY/RsmZ sRNAsActivatorPMID: 24169572

SuhB (PA3818)Indirect repressorPMID: 24169572

AmrZ (PA3385)Activator: PA0082 (TssA)PMID: 24603766

Adaptors list

Adaptor identified
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  Homolog
1PA0094114611..115045 [-], 43515595292hypothetical protein PA0094
Data from experimental literature

Download FASTA format files
Proteins        Genes
(1) Forster A et al. (2014). Coevolution of the ATPase ClpV, the Sheath Proteins TssB and TssC and the Accessory Protein TagJ/HsiE1 Distinguishes Type VI Secretion Classes. J Biol Chem. 289(47):33032-43. [PudMed:25305017] experimental in_silico
(2) Hachani A et al. (2014). The VgrG proteins are "A la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 289(25):17872-17884. [PudMed:24794869] experimental
(3) Whitney JC et al. (2014). Genetically distinct pathways guide effector export through the type VI secretion system. Mol Microbiol. 92(3):529-42. [PudMed:24589350] experimental
(4) Robb CS et al. (2013). Structure of the T6SS lipoprotein TssJ1 from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 69(Pt 6):607-10. [PudMed:23722835] experimental in_silico
(5) Lu D et al. (2013). Expression, purification and preliminary crystallographic analysis of the T6SS effector protein Tse3 from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 69(Pt 5):524-7. [PudMed:23695568] experimental
(6) Basler M et al. (2013). Tit-for-Tat: Type VI Secretion System Counterattack during Bacterial Cell-Cell Interactions. Cell. 152(4):884-94. [PudMed:23415234] experimental
(7) Leroux M et al. (2012). Quantitative single-cell characterization of bacterial interactions reveals type VI secretion is a double-edged sword. Proc Natl Acad Sci U S A. 109(48):19804-9. [PudMed:23150540] experimental
(8) Lossi NS et al. (2012). The archetype Pseudomonas aeruginosa proteins TssB and TagJ form a novel subcomplex in the bacterial type VI secretion system. Mol Microbiol. 86(2):437-56. [PudMed:22906320]
(9) Chou S et al. (2012). Structure of a Peptidoglycan Amidase Effector Targeted to Gram-Negative Bacteria by the Type VI Secretion System. Cell Rep. 1(6):656-64. [PudMed:22813741]
(10) Casabona MG et al. (2012). An ABC transporter and an outer membrane lipoprotein participate in posttranslational activation of type VI secretion in Pseudomonas aeruginosa. Environ Microbiol. 15(2):471-86. [PudMed:22765374] experimental
(11) Zhang H et al. (2012). Crystal structure of type VI effector Tse1 from Pseudomonas aeruginosa. FEBS Lett. 586(19):3193-9. [PudMed:22750141]
(12) Ding J et al. (2012). Structural Insights into the Pseudomonas aeruginosa Type VI Virulence Effector Tse1 Bacteriolysis and Self-protection Mechanisms. J Biol Chem. 287(32):26911-20. [PudMed:22700987]
(13) Kefala K et al. (2012). Purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal fragment of the MvfR protein from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 68(Pt 6):695-7. [PudMed:22684073]
(14) Sana TG et al. (2012). The Second Type VI Secretion System of Pseudomonas aeruginosa Strain PAO1 Is Regulated by Quorum Sensing and Fur and Modulates Internalization in Epithelial Cells. J Biol Chem. 287(32):27095-105. [PudMed:22665491] experimental
(15) Li M et al. (2012). Structural basis for type VI secretion effector recognition by a cognate immunity protein. PLoS Pathog. 8(4):e1002613. [PudMed:22511866]
(16) Zou T et al. (2012). Crystal structure of Pseudomonas aeruginosa Tsi2 reveals a stably folded superhelical antitoxin. J Mol Biol. 417(4):351-61. [PudMed:22310046]
(17) Silverman JM et al. (2011). Separate inputs modulate phosphorylation-dependent and -independent type VI secretion activation. Mol Microbiol. 82(5):1277-90. [PudMed:22017253] experimental
(18) Goldova J et al. (2011). A eukaryotic-type signalling system of Pseudomonas aeruginosa contributes to oxidative stress resistance, intracellular survival and virulence. BMC Genomics. 12:437. [PudMed:21880152]
(19) Lossi NS et al. (2011). Structure-function analysis of HsiF, a gp25-like component of the type VI secretion system, in Pseudomonas aeruginosa. Microbiology. 157(Pt 12):3292-305. [PudMed:21873404]
(20) Russell AB et al. (2011). Type VI secretion delivers bacteriolytic effectors to target cells. Nature. 475(7356):343-7. [PudMed:21776080] experimental
(21) Osipiuk J et al. (2011). Crystal structure of secretory protein Hcp3 from Pseudomonas aeruginosa. J Struct Funct Genomics. 12(1):21-6. [PudMed:21476004]
(22) Barret M et al. (2011). Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537] in_silico
(23) Hachani A et al. (2011). Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. J Biol Chem. 286(14):12317-27. [PudMed:21325275] experimental
(24) Bleves S et al. (2010). Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons. Int J Med Microbiol. 300(8):534-43. [PudMed:20947426]
(25) Hood RD et al. (2010). A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Cell Host Microbe. 7(1):25-37. [PudMed:20114026] experimental
(26) Hsu F et al. (2009). TagR promotes PpkA-catalysed type VI secretion activation in Pseudomonas aeruginosa. Mol Microbiol. 72(5):1111-25. [PudMed:19400797] experimental
(27) Mougous JD et al. (2007). Threonine phosphorylation post-translationally regulates protein secretion in Pseudomonas aeruginosa. Nat Cell Biol. 9(7):797-803. [PudMed:17558395]
(28) Mougous JD et al. (2006). A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science. 312(5779):1526-30. [PudMed:16763151] experimental
experimental This literature contains experimental investigation
in_silico This literature contains bioinformatics investigation