I. Summary

SecReT6 ID	300
Strain	Vibrio fischeri MJ11
Replicon	chromosome II [Browse all T6SS(s) in this replicon]
Accession	NC_011186
Location	879228..921842
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	2	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	VFMJ11_A0803	874592..876883 [-], 2292	197337550	serine protease
2	VFMJ11_A0804	877160..877762 [+], 603	197336823	lipoprotein, putative
3	VFMJ11_A0805	877772..879226 [+], 1455	197337793	type VI secretion system FHA domain protein	Fha
4	VFMJ11_A0806	879228..879728 [+], 501	197337278	type VI secretion lipoprotein, family	TssJ
5	VFMJ11_A0807	879740..881065 [+], 1326	197337818	type VI secretion protein, family	TssK
6	VFMJ11_A0808	881062..881853 [+], 792	197337045	type IV / VI secretion system protein, DotU family	TssL
7	VFMJ11_A0809	881894..883405 [+], 1512	197336690	hypothetical protein	PAAR
8	VFMJ11_A0810	883408..884181 [+], 774	197337184	hypothetical protein
9	VFMJ11_A0811	884191..884826 [+], 636	197336896	hypothetical protein
10	VFMJ11_A0812	884836..885327 [+], 492	197337494	hypothetical protein
11	VFMJ11_A0813	885351..885962 [+], 612	197337132	hypothetical protein
12	VFMJ11_A0814	886052..896644 [-], 10593	197337670	lipoprotein, putative
13	VFMJ11_A0815	896890..897819 [+], 930	197337115	M23 peptidase domain protein
14	VFMJ11_A0816	898055..899359 [+], 1305	197337689	ImpA-related N-terminal family protein	TssA
15	VFMJ11_A0817 (icmF_2)	899394..902792 [+], 3399	197336804	type VI secretion protein IcmF	TssM
16	VFMJ11_A0818	902828..903277 [+], 450	197337186	transcriptional regulator
17	VFMJ11_A0819	903349..904347 [-], 999	197336905	type VI secretion protein, family	TssG
18	VFMJ11_A0820 (vasA_2)	904344..906092 [-], 1749	197337360	type VI secretion protein, family	TssF
19	VFMJ11_A0821	906105..906515 [-], 411	197337170	type VI secretion system lysozyme-related protein	TssE
20	VFMJ11_A0822	906575..908053 [-], 1479	197337884	type VI secretion protein, EvpB/family	TssC
21	VFMJ11_A0823	908062..908571 [-], 510	197337608	type VI secretion protein, family	TssB
22	VFMJ11_A0824	908630..910189 [-], 1560	197337659	type VI secretion-associated protein, family	TssA
23	VFMJ11_A0825	910191..912176 [-], 1986	197336793	serine-threonine protein kinase
24	VFMJ11_A0826	912330..913271 [-], 942	197337337	hypothetical protein
25	VFMJ11_A0827	913271..914248 [-], 978	197337887	hypothetical protein
26	VFMJ11_A0828	914229..915554 [-], 1326	197337169	hypothetical protein
27	VFMJ11_A0829	915563..916057 [-], 495	197337717	hypothetical protein
28	VFMJ11_A0830	916044..918122 [-], 2079	197336955	Rhs element Vgr family protein	TssI
29	VFMJ11_A0831	918192..918710 [-], 519	197337277	BfdA	TssD
30	VFMJ11_A0832 (clpV_2)	919176..921842 [+], 2667	197336709	type VI secretion ATPase, ClpV1 family	TssH
31	VFMJ11_A0833	921829..923439 [+], 1611	197337394	nitric oxide reductase regulator
32	VFMJ11_A0834	923440..923940 [+], 501	197336751	lipoprotein, putative

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

VEPGS_0021 , PSPTO_5435

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	VFMJ11_A0831	918192..918710 [-], 519	197337277	BfdA	VEPGS_0021
2	VFMJ11_A1070	1186939..1187457 [-], 519	197337584	hemolysin co-regulated protein	PSPTO_5435

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Proteins        Genes

N/A

N/A