I. Summary

SecReT6 ID	301
Strain	Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_011205
Location	305501..331344
Comment	Involved in colonization of the avian and murine hosts
Function	anti-eukaryotic; virulence
T6SS Type	Type i; subtype i3
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	0
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	SeD_A0283 (mltD)	300759..301979 [-], 1221	198243785	membrane-bound lytic murein transglycosylase D
2	SeD_A0284 (gloB)	302198..302953 [-], 756	198244534	hydroxyacylglutathione hydrolase
3	SeD_A0285	302988..303710 [+], 723	198243385	methyltransferase
4	SeD_A0286 (rnhA)	303707..304174 [-], 468	198242273	ribonuclease H
5	SeD_A0287 (dnaQ)	304229..304969 [+], 741	198244203	DNA polymerase III subunit epsilon
6	SeD_A0289	305501..306556 [-], 1056	198245787	ImpA-related protein	TssA
7	SeD_A0290	306567..307502 [-], 936	198245445	SciB protein	TssG
8	SeD_A0292	309412..309906 [-], 495	198241906	hypothetical protein	TssE
9	SeD_A0293	309903..310727 [-], 825	198242047	SciE protein	TagJ
10	SeD_A0294	310714..311604 [-], 891	198242192	hypothetical protein
11	SeD_A0295	311984..314623 [+], 2640	198243880	ATPase	TssH
12	SeD_A0296	314723..315265 [+], 543	198244612	hypothetical protein	TssB
13	SeD_A0297	315289..316797 [+], 1509	198243093	SciI protein	TssC
14	SeD_A0298	317071..317250 [+], 180	198243970	hypothetical protein
15	SeD_A0299	317263..317709 [+], 447	198244319	hypothetical protein
16	SeD_A0300	317961..318446 [+], 486	198244078	hemolysin-coregulated protein	TssD
17	SeD_A0301	318558..319049 [+], 492	198244689	SciN protein	TssJ
18	SeD_A0302	319053..320396 [+], 1344	198245302	hypothetical protein	TssK
19	SeD_A0303	320393..321697 [+], 1305	198245916	hypothetical protein	TssL
20	SeD_A0304	321702..322475 [+], 774	198242259	inner membrane protein
21	SeD_A0305	322753..323109 [+], 357	198242773	SciR protein
22	SeD_A0306	323143..327012 [+], 3870	198242462	SciS protein	TssM
23	SeD_A0307	327012..327800 [+], 789	198243222	hypothetical protein
24	SeD_A0308	327827..328213 [+], 387	198242568	hypothetical protein
25	SeD_A0309	328240..328758 [+], 519	198244209	hypothetical protein
26	SeD_A0310	329155..331344 [+], 2190	198243551	Rhs family protein	TssI
27	SeD_A0311	331368..331814 [+], 447	198242189	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

SciK , STY2606

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	SeD_A0300	317961..318446 [+], 486	198244078	hemolysin-coregulated protein	SciK
2	SeD_A2728	2608078..2608446 [+], 369	198244734	hypothetical protein	STY2606

Download FASTA format files

Proteins        Genes

Homology Immunity protein list

STY2607

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	SeD_A2729	2608446..2608964 [+], 519	198242944	hypothetical protein	STY2607

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Proteins        Genes

(1) Pezoa D et al. (2014). Only one of the two type VI secretion systems encoded in the Salmonella enterica serotype Dublin genome is involved in colonization of the avian and murine hosts. Vet Res. 45(1):2. [PudMed:24405577]

(2) Blondel CJ et al. (2009). Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics. 10:354. [PudMed:19653904]

This literature contains experimental investigation

This literature contains bioinformatics investigation