I. Summary

SecReT6 ID	303
Strain	Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_011274
Location	1114451..1137704
Comment	Colonisation of chicken ileum, caeca, liver and spleen
Function	anti-eukaryotic
T6SS Type	Type i; subtype i1
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	2	1	1	2	1	1	0	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	SG1023	1110982..1111287 [-], 306	205352283	hypothetical protein
2	SG1025	1112125..1112583 [-], 459	205352284	hypothetical protein
3	SG1026	1112707..1113078 [-], 372	205352285	hypothetical protein
4	SG1028	1113546..1114436 [-], 891	205352286	DNA-binding protein
5	SG1029	1114451..1114930 [-], 480	205352287	hypothetical protein	TssD
6	SG1030	1114918..1116549 [-], 1632	205352288	hypothetical protein	TssA
7	SG1031	1116332..1119853 [-], 3522	205352289	hypothetical protein	TssM
8	SG1032	1119872..1121167 [-], 1296	205352290	hypothetical protein	TssA
9	SG1033	1121286..1122023 [-], 738	205352291	hypothetical protein
10	SG1034	1122020..1124683 [-], 2664	205352292	clpB-protease	TssH
11	SG1035	1124696..1125454 [-], 759	205352293	hypothetical protein	TssL
12	SG1036	1125459..1126790 [-], 1332	205352294	hypothetical protein	TssK
13	SG1037	1126793..1127326 [-], 534	205352295	lipoprotein	TssJ
14	SG1038	1127323..1128621 [-], 1299	205352296	hypothetical protein	Fha
15	SG1039	1128634..1129728 [-], 1095	205352297	hypothetical protein	TssG
16	SG1040	1129686..1131599 [-], 1914	205352298	hypothetical protein	TssF
17	SG1041	1131957..1133429 [-], 1473	205352299	hypothetical protein	TssC
18	SG1042	1133720..1134232 [-], 513	205352300	hypothetical protein	TssB
19	SG1043	1134853..1135371 [+], 519	205352301	hypothetical protein	TssD
20	SG1044	1135593..1137704 [+], 2112	205352302	RHS family protein	TssI
21	SG1045	1137806..1141114 [+], 3309	205352303	RHS family protein	PAAR
22	SG1047	1142197..1142601 [+], 405	205352304	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

ECA4275 , STY2606, Hcp

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	SG1043	1134853..1135371 [+], 519	205352301	hypothetical protein	ECA4275
2	SG2405	2471848..2472216 [+], 369	205353488	hypothetical protein	STY2606
3	SG3025	3156314..3156799 [-], 486	205354051	hypothetical protein	Hcp

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Proteins        Genes

Homology Immunity protein list

STY2607

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	SG2406	2472216..2472734 [+], 519	205353489	lipoprotein	STY2607

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Proteins        Genes

(1) Pezoa D et al. (2013). The Type VI Secretion System Encoded in SPI-6 Plays a Role in Gastrointestinal Colonization and Systemic Spread of Salmonella enterica serovar Typhimurium in the Chicken. PLoS One. 8(5):e63917. [PudMed:23691117]

(2) Blondel CJ et al. (2013). The Type VI Secretion System encoded in SPI-19 is required for Salmonella Gallinarum survival within infected macrophages. Infect Immun. 81(4):1207-20. [PudMed:23357385]

(3) Silva CA et al. (2012). Infection of mice by Salmonella enterica serovar Enteritidis involves additional genes that are absent in the genome of serovar Typhimurium. Infect Immun. 80(2):839-49. [PudMed:22083712]

(4) Blondel CJ et al. (2010). Contribution of the type VI secretion system encoded in SPI-19 to chicken colonization by Salmonella enterica serotypes Gallinarum and Enteritidis. PLoS One. 5(7):e11724. [PudMed:20661437]

(5) Blondel CJ et al. (2009). Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics. 10:354. [PudMed:19653904]

This literature contains experimental investigation

This literature contains bioinformatics investigation