I. Summary

SecReT6 ID	303
Strain	Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_011274
Location	1114451..1137704
Comment	Colonisation of chicken ileum, caeca, liver and spleen
Function	anti-eukaryotic
T6SS Type	Type i; subtype i1
Experimental investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	2	1	1	2	1	1	0	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	SG_RS05180	1109709..1110815 [+], 1107	pseudogene	Gfo/Idh/MocA family oxidoreductase
2	SG_RS05185	1110982..1111275 [-], 294	446036546	hypothetical protein
3	SG_RS05190	1111306..1111524 [+], 219	446803361	hypothetical protein
4	SG_RS05200	1112125..1112583 [-], 459	446546026	DUF1311 domain-containing protein
5	SG_RS05205	1112707..1113078 [-], 372	447150137	hypothetical protein
6	SG_RS22830	1113087..1113530 [-], 444	1102634496	hypothetical protein
7	SG_RS05210	1113546..1114418 [-], 873	446170355	LysM peptidoglycan-binding domain-containing protein
8	SG_RS05215	1114451..1114930 [-], 480	447207708	Hcp family type VI secretion system effector
9	SG_RS05220	1114918..1116321 [-], 1404	445998214	type VI secretion system ImpA family N-terminal domain-containing protein
10	SG_RS05225 (tssM)	1116332..1119793 [-], 3462	447059852	type VI secretion system membrane subunit TssM
11	SG_RS05230 (tssA)	1119872..1121284 [-], 1413	pseudogene	type VI secretion system protein TssA
12	SG_RS05235 (tagO)	1121286..1122023 [-], 738	446011293	type VI secretion system-associated protein TagO
13	SG_RS05240	1122020..1124683 [-], 2664	446536954	AAA family ATPase
14	SG_RS05245	1124696..1125454 [-], 759	446266123	type IVB secretion system protein IcmH/DotU
15	SG_RS05250 (tssK)	1125459..1126790 [-], 1332	446168599	type VI secretion system baseplate subunit TssK
16	SG_RS05255 (tssJ)	1126793..1127296 [-], 504	1524033542	type VI secretion system lipoprotein TssJ
17	SG_RS05260 (tagH)	1127323..1128609 [-], 1287	446719629	type VI secretion system-associated FHA domain protein TagH
18	SG_RS05265 (tssG)	1128634..1129722 [-], 1089	446431194	type VI secretion system baseplate subunit TssG
19	SG_RS05270 (tssF)	1129686..1131539 [-], 1854	446316014	type VI secretion system baseplate subunit TssF
20	SG_RS05275 (tssE)	1131544..1131960 [-], 417	446534352	type VI secretion system baseplate subunit TssE
21	SG_RS05280 (tssC)	1131957..1133429 [-], 1473	445980146	type VI secretion system contractile sheath large subunit
22	SG_RS05290 (tssB)	1133720..1134223 [-], 504	445953397	type VI secretion system contractile sheath small subunit
23	SG_RS05300	1134853..1135371 [+], 519	447065710	Hcp family type VI secretion system effector
24	SG_RS05305	1135593..1137704 [+], 2112	446025594	type VI secretion system tip protein VgrG
25	SG_RS05310	1137806..1141606 [+], 3801	pseudogene	RHS repeat protein
26	SG_RS24050	1141623..1142204 [+], 582	487407310	hypothetical protein
27	SG_RS05320	1142197..1142601 [+], 405	446545186	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

ECA4275 , STY2606, Hcp

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog

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Proteins        Genes

Homology Immunity protein list

STY2607

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog

Download FASTA format files

Proteins        Genes

(1) Pezoa D et al. (2013). The Type VI Secretion System Encoded in SPI-6 Plays a Role in Gastrointestinal Colonization and Systemic Spread of Salmonella enterica serovar Typhimurium in the Chicken. PLoS One. 8(5):e63917. [PudMed:23691117]

(2) Blondel CJ et al. (2013). The Type VI Secretion System encoded in SPI-19 is required for Salmonella Gallinarum survival within infected macrophages. Infect Immun. 81(4):1207-20. [PudMed:23357385]

(3) Silva CA et al. (2012). Infection of mice by Salmonella enterica serovar Enteritidis involves additional genes that are absent in the genome of serovar Typhimurium. Infect Immun. 80(2):839-49. [PudMed:22083712]

(4) Blondel CJ et al. (2010). Contribution of the type VI secretion system encoded in SPI-19 to chicken colonization by Salmonella enterica serotypes Gallinarum and Enteritidis. PLoS One. 5(7):e11724. [PudMed:20661437]

(5) Blondel CJ et al. (2009). Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics. 10:354. [PudMed:19653904]

This literature contains experimental investigation

This literature contains bioinformatics investigation