I. Summary

SecReT6 ID	326
Strain	Pseudomonas aeruginosa LESB58
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_011770
Location	4045586..4064561
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	0	1	0	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

11

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	PLES_36511	4041135..4041455 [+], 321	218892369	hypothetical protein
2	PLES_36521	4041512..4042069 [+], 558	218892370	hypothetical protein
3	PLES_36531 (folE)	4042147..4042692 [+], 546	218892371	GTP cyclohydrolase I
4	PLES_36541	4042757..4043218 [-], 462	218892372	hypothetical protein
5	PLES_36551	4043464..4043844 [+], 381	218892373	putative enzyme
6	PLES_36561 (stk1)	4043872..4044861 [-], 990	218892374	serine-threonine kinase Stk1	Stk1
7	PLES_36571 (stp1)	4044858..4045586 [-], 729	218892375	serine/threonine phosphoprotein phosphatase Stp1	Stp1
8	PLES_36581	4045586..4049113 [-], 3528	218892376	hypothetical protein	TssM
9	PLES_36591	4049129..4049998 [-], 870	218892377	putative outer membrane protein	TssL
10	PLES_36601	4050001..4051332 [-], 1332	218892378	hypothetical protein	TssK
11	PLES_36611	4051329..4051835 [-], 507	218892379	putative lipoprotein	TssJ
12	PLES_36621	4051841..4053034 [-], 1194	218892380	hypothetical protein	Fha
13	PLES_36631	4053052..4053192 [-], 141	218892381	hypothetical protein
14	PLES_36641	4053281..4054792 [-], 1512	218892382	putative transcriptional regulator	Sfa2
15	PLES_36651	4054803..4057436 [-], 2634	218892383	putative ClpA/B-type protease	TssH
16	PLES_36661	4057443..4058450 [-], 1008	218892384	hypothetical protein	TssG
17	PLES_36671	4058414..4059994 [-], 1581	218892385	hypothetical protein	TssF
18	PLES_36681	4060539..4060946 [-], 408	218892386	hypothetical protein	TssE
19	PLES_36691	4060959..4062434 [-], 1476	218892387	hypothetical protein	TssC
20	PLES_36701	4062463..4062969 [-], 507	218892388	hypothetical protein	TssB
21	PLES_36711	4063005..4064561 [-], 1557	218892389	hypothetical protein	TssA
22	PLES_36721	4065733..4066335 [-], 603	218892390	putative glutathione S-transferase
23	PLES_36731	4066391..4067557 [-], 1167	218892391	putative aminotransferase
24	PLES_36741	4067639..4068115 [+], 477	218892392	putative transcriptional regulator
25	PLES_36751	4068166..4068801 [-], 636	218892393	hypothetical protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PSPTO_5435 , PSPTO_5435, PSPTO_5435

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	PLES_02591(hcpC)	289993..290511 [-], 519	218889003	secreted protein Hcp	PSPTO_5435
2	PLES_38981(hcpA)	4308128..4308646 [+], 519	218892612	secreted protein Hcp	PSPTO_5435
3	PLES_56611(hcpB)	6268805..6269323 [-], 519	218894369	secreted protein Hcp	PSPTO_5435

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Proteins        Genes

N/A

(1) Barret M et al. (2011). Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537]

This literature contains bioinformatics investigation