I. Summary

SecReT6 ID	334
Strain	Azotobacter vinelandii DJ
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_012560
Location	5141501..5160424
Comment
Function	N/A
T6SS Type	Type i; subtype i4a
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	1	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	0	1	1

12

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	Avin_50690 (modG)	5137140..5137568 [+], 429	226947059	Mo processing, homeostasis
2	Avin_50700	5137651..5138601 [+], 951	226947060	ABC transporter ATP-binding protein
3	Avin_50710	5138555..5139448 [-], 894	226947061	ABC type tungstate transporter, permease
4	Avin_50720	5139591..5140286 [-], 696	226947062	ABC transporter permease
5	Avin_50730 (modA3)	5140283..5141059 [-], 777	226947063	molybdate ABC transporter substrate-binding protein
6	Avin_50750	5141501..5142592 [-], 1092	226947064	ImpA-like protein	TssA
7	Avin_50760	5142608..5146426 [-], 3819	226947065	hypothetical protein	TssM
8	Avin_50770	5146423..5147187 [-], 765	226947066	hypothetical protein	TssL
9	Avin_50780	5147205..5148530 [-], 1326	226947067	hypothetical protein	TssK
10	Avin_50790	5148576..5149073 [-], 498	226947068	hypothetical protein
11	Avin_50800	5149261..5149824 [+], 564	226947069	hypothetical protein	TssB
12	Avin_50810	5149857..5151338 [+], 1482	226947070	hypothetical protein	TssC
13	Avin_50820	5151416..5151913 [+], 498	226947071	hypothetical protein	TssD
14	Avin_50830	5151929..5152381 [+], 453	226947072	hypothetical protein	TssE
15	Avin_50840	5152365..5154158 [+], 1794	226947073	hypothetical protein	TssF
16	Avin_50850	5154122..5155144 [+], 1023	226947074	hypothetical protein	TssG
17	Avin_50860	5155147..5157675 [+], 2529	226947075	AAA ATPase	TssH
18	Avin_50870	5158418..5160424 [+], 2007	226947076	Rhs element Vgr protein	TssI
19	Avin_50880	5160435..5160980 [+], 546	226947077	hypothetical protein
20	Avin_50890	5160977..5161309 [-], 333	226947078	hypothetical protein
21	Avin_50900	5161556..5161951 [-], 396	226947079	nitrogen fixation-like protein
22	Avin_50910 (nafY)	5161948..5162679 [-], 732	226947080	nitrogen fixation-like protein subunit gamma
23	Avin_50920 (rnfH)	5162704..5162964 [-], 261	226947081	RnfABCDGE type electron transport complex subunit H
24	Avin_50930 (rnfE1)	5162986..5163702 [-], 717	226947082	RnfABCDGE type electron transport complex subunit E
25	Avin_50940 (rnfG1)	5163716..5164405 [-], 690	226947083	RnfABCDGE type electron transport complex subunit G

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

Hcp

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	Avin_50820	5151416..5151913 [+], 498	226947071	hypothetical protein	Hcp

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Proteins        Genes

N/A

N/A