I. Summary

SecReT6 ID	336
Strain	Vibrio cholerae MJ-1236
Replicon	chromosome 2 [Browse all T6SS(s) in this replicon]
Accession	NC_012667
Location	132888..156676
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	0	1	1	2	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

13

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	VCD_000116	127946..128830 [-], 885	229605176	D-ribose transporter subunit RbsB
2	VCD_000117 (rbsC)	128885..129886 [-], 1002	229605177	ribose ABC transporter permease protein
3	VCD_000118	129883..131385 [-], 1503	229605178	D-ribose transporter ATP binding protein
4	VCD_000119	131412..131882 [-], 471	229605179	D-ribose pyranase
5	VCD_000120	131883..132014 [+], 132	229605180	hypothetical protein
6	VCD_000121	132115..132375 [+], 261	229605181	hypothetical protein
7	VCD_000122	132888..135941 [-], 3054	229605182	VgrG protein	TssI
8	VCD_000123	136194..137459 [-], 1266	229605183	type VI secretion-related protein VasL	TssA
9	VCD_000124	137507..141052 [-], 3546	229605184	IcmF-related protein	TssM
10	VCD_000125	141068..142477 [-], 1410	229605185	hypothetical protein	TssA
11	VCD_000126	142487..143170 [-], 684	229605186	type VI secretion protein VasI	VCA0118
12	VCD_000127	143140..144732 [-], 1593	229605187	sigma-54 dependent transcriptional regulator	VasH
13	VCD_000128	144735..147344 [-], 2610	229605188	ClpB protein	TssH
14	VCD_000129	147370..148143 [-], 774	229605189	ImpK/VasF outer membrane protein	TssL
15	VCD_000130	148146..149480 [-], 1335	229605190	hypothetical protein	TssK
16	VCD_000131	149487..149963 [-], 477	229605191	type VI secretion lipoprotein/VasD	TssJ
17	VCD_000132	149966..151453 [-], 1488	229605192	hypothetical protein	Fha
18	VCD_000133	151456..152472 [-], 1017	229605193	hypothetical protein	TssG
19	VCD_000134	152436..154205 [-], 1770	229605194	protein ImpG/VasA	TssF
20	VCD_000135	154211..154648 [-], 438	229605195	hypothetical protein	TssE
21	VCD_000136	154651..156129 [-], 1479	229605196	hypothetical protein	TssC
22	VCD_000137	156170..156676 [-], 507	229605197	hypothetical protein	TssB
23	VCD_000138	157108..158103 [-], 996	229605198	hypothetical protein
24	VCD_000139	158096..158380 [-], 285	229605199	uropathogenic specific protein	PAAR
25	VCD_000140	159119..160831 [+], 1713	229605200	pseudouridylate synthase LSU rRNA-specific

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

VCA0123 , VCA0118, VCA0020, VCA0018, VCA0017, VCA0285, VC1418, VC1416, ABG57132

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	VCD_000122	132888..135941 [-], 3054	229605182	VgrG protein	VCA0123
2	VCD_000126	142487..143170 [-], 684	229605186	type VI secretion protein VasI	VCA0118
3	VCD_000213	246869..250126 [-], 3258	229605273	hypothetical protein	VCA0020
4	VCD_000215	251010..253094 [-], 2085	229605275	VgrG protein	VCA0018
5	VCD_000216	253222..253740 [-], 519	229605276	Hcp protein	VCA0017
6	VCD_000989	1054475..1055146 [-], 672	229606039	hypothetical protein	VCA0285
7	VCD_002929	2127535..2129460 [-], 1926	229608011	lipase family protein	VC1418
8	VCD_002931	2130302..2133793 [-], 3492	229608013	VgrG protein	VC1416
9	VCD_002932	2133921..2134439 [-], 519	229608014	Hcp protein	ABG57132

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Proteins        Genes

Homology Immunity protein list

VCA0021, VC1419

Immunity protein identified
#	Locus tag(Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	VCD_000212	246140..246868 [-], 729	229605272	hypothetical protein	VCA0021
2	VCD_002928	2126795..2127538 [-], 744	229608010	hypothetical protein	VC1419

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Proteins        Genes

N/A