I. Summary

SecReT6 ID	337
Strain	Tolumonas auensis DSM 9187
Replicon	chromosome [Browse all T6SS(s) in this replicon]
Accession	NC_012691
Location	212136..234045
Comment
Function	N/A
T6SS Type	Type i; subtype i1
Bioinformatics investigation has been performed on this T6SS.

II. T6SS components

Component	TssH	TssD	TssL	TssI	TssA	TssB	TssC	TssE	TssF
Number	1	0	1	1	1	1	1	1	1
Component	TssG	TssJ	TssK	TssM
Number	1	1	1	1

III. genome coordinates of the T6SS gene cluster
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Note
1	Tola_0191	210792..211256 [-], 465	237806967	YbaK/prolyl-tRNA synthetase associated domain-containing protein
2	Tola_0192	211572..211853 [-], 282	237806968	hypothetical protein
3	Tola_0193	212136..213677 [+], 1542	237806969	type VI secretion-associated protein	TssA
4	Tola_0194	213691..214185 [+], 495	237806970	type VI secretion protein	TssB
5	Tola_0195	214204..215679 [+], 1476	237806971	type VI secretion protein EvpB	TssC
6	Tola_0196	215682..216095 [+], 414	237806972	type VI secretion system lysozyme-like protein	TssE
7	Tola_0197	216085..217842 [+], 1758	237806973	type VI secretion protein	TssF
8	Tola_0198	217806..218825 [+], 1020	237806974	type VI secretion protein	TssG
9	Tola_0199	218812..221421 [+], 2610	237806975	type VI secretion ATPase, ClpV1 family	TssH
10	Tola_0200	221430..222434 [+], 1005	237806976	Fis family sigma-54 specific transcriptional regulator	Sfa2
11	Tola_0201	222434..222571 [+], 138	237806977	hypothetical protein
12	Tola_0202	222575..223870 [+], 1296	237806978	FHA domain-containing protein	Fha
13	Tola_0203	223876..224427 [+], 552	237806979	type VI secretion lipoprotein	TssJ
14	Tola_0204	224501..225844 [+], 1344	237806980	type VI secretion protein	TssK
15	Tola_0205	225846..226712 [+], 867	237806981	DotU family type IV / VI secretion system protein	TssL
16	Tola_0206	226709..230230 [+], 3522	237806982	type VI secretion protein IcmF	TssM
17	Tola_0207	230238..230972 [+], 735	237806983	protein serine/threonine phosphatase
18	Tola_0208	231013..231915 [+], 903	237806984	serine/threonine protein kinase
19	Tola_0209	231934..234045 [+], 2112	237806985	ImpA family type VI secretion-associated protein	TssI
20	Tola_0210	234171..235781 [+], 1611	237806986	glutamate/cysteine ligase
21	Tola_0211	235816..236493 [-], 678	237806987	glutathione S-transferase
22	Tola_0212	236831..238168 [+], 1338	237806988	citrate carrier protein

 Genes in the 5-kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any. In the 'Note' column,if available, '(e)' denotes effector while '(i)' for immunity protein

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Proteins        Genes

V. Investigation of the genomic context of the T6SS gene cluster.

1. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the mobile genetic elements database, ACLAME.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

2. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against the virulence factor database, VFDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

3. BLASTp searches of the proteins encoded by T6SS gene cluster and its flanking regions against against the antibiotic resistance database, ARDB.

Job_id: Replicon RefSeq: T6SS Region Start (bp): T6SS Region End (bp): Upstream Region (kb): Downstream Region (kb):

Homology Effector list

PSPTO_5435

Effector identified
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Homolog
1	Tola_0342	385297..385815 [-], 519	237807118	type VI secretion system effector	PSPTO_5435

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Proteins        Genes

N/A

N/A